Isolation and Characterization of Bacillus sp. Producing Broad-Spectrum Antibiotics Against Human and Plant Pathogenic Fungi

Chen, Na; Jin, Min; Qu, Hongmei; Chen, Zhiqiang; Chen, Zhao-li; Qiu, Zhigang; Wang, Xinwei; Lǐ, Jùnwén

doi:10.4014/jmb.1107.07021

Cited by 12 publications

(7 citation statements)

References 26 publications

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“…Previous studies have shown that B. amyloliquefaciens strains possess significant antifungal activity against F. oxysporum , which suppresses Fusarium wilt in various crops, such as watermelon and tomato [ 50 , 52 , 53 , 54 , 55 , 56 , 57 ]. Moreover, the cell-free supernatant of the B. amyloliquefaciens DHA55 culture displayed significant growth-inhibiting potential against different pathogenic fungi, including Fon , Db , Fg , Ss , and Rs ( Figure 7 ), similar to observations that cell-free supernatants of B. subtilis and B. amyloliquefaciens inhibited the mycelial growth and/or spore germination of Macrophomina phaseolina , the causal agent of charcoal rot in soybean and common bean, and Fusarium semitectum , which causes rice dirty panicle disease, respectively [ 58 , 59 , 60 ]. Moreover, lipopeptide extract of B. amyloliquefaciens DHA55 at 100 µg/mL significantly inhibited Fon growth ( Figure 7 C), which was consistent with previous observations regarding B. methyltrophicus TEB1 and B. amyloliquefaciens SYBC H47 [ 49 , 61 , 62 , 63 ].…”

Section: Discussionsupporting

confidence: 76%

Suppression of Fusarium Wilt in Watermelon by Bacillus amyloliquefaciens DHA55 through Extracellular Production of Antifungal Lipopeptides

Al-Mutar

Alzawar

Noman

et al. 2023

JoF

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Fusarium wilt caused by Fusarium oxysporum f. sp. niveum is one of the most devastating fungal diseases affecting watermelon (Citrullus lanatus L.). The present study aimed to identify potent antagonistic bacterial strains with substantial antifungal activity against F. oxysporum f. sp. niveum and to explore their potential for biocontrol of Fusarium wilt in watermelon. Out of 77 isolates from watermelon rhizosphere, six bacterial strains—namely, DHA4, DHA6, DHA10, DHA12, DHA41, and DHA55—exhibited significant antifungal activity against F. oxysporum f. sp. niveum, as well as other phytopathogenic fungi, including Didymella bryoniae, Sclerotinia sclerotiorum, Fusarium graminearum, and Rhizoctonia solani. These Gram-positive, rod-shaped, antagonistic bacterial strains were able to produce exo-enzymes (e.g., catalase, protease, and cellulase), siderophore, and indole-3-acetic acid and had the ability to solubilize phosphate. In greenhouse experiments, these antagonistic bacterial strains not only promoted plant growth but also suppressed Fusarium wilt in watermelon. Among these strains, DHA55 was the most effective, achieving the highest disease suppression of 74.9%. Strain DHA55 was identified as Bacillus amyloliquefaciens based on physiological, biochemical, and molecular characterization. B. amyloliquefaciens DHA55 produced various antifungal lipopeptides, including iturin, surfactin, and fengycin, that showed significant antifungal activities against F. oxysporum f. sp. niveum. Microscopic observations revealed that B. amyloliquefaciens DHA55 exhibited an inhibitory effect against F. oxysporum f. sp. niveum on the root surface of watermelon plants. These results demonstrate that B. amyloliquefaciens DHA55 can effectively promote plant growth and suppress the development of watermelon Fusarium wilt, providing a promising agent for the biocontrol of Fusarium wilt in watermelon.

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Section: Discussionsupporting

confidence: 76%

Suppression of Fusarium Wilt in Watermelon by Bacillus amyloliquefaciens DHA55 through Extracellular Production of Antifungal Lipopeptides

Al-Mutar

Alzawar

Noman

et al. 2023

JoF

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“…The structures of the compounds were then confirmed using 1 H NMR ( Figures S1 {Supplement 1} through S5 {Supplement 5}). All of the isolated compounds were small cyclic peptides ( Figure 6); the characterization data for each compound were in good agreement with the literature (Chen et al, 2009;Nakamura et al, 2006;Stark and Hofmann, 2005). 195.1128195.…”

Section: Purification and Identification Of Antifungal Metabolitessupporting

confidence: 86%

Characterization and Isolation of Peptide Metabolites of an Antifungal Bacterial Isolate Identified as Bacillus Amyloliquefaciens Subspecies Plantarum Strain Fzb42

Adibi¹,

Rees²,

McCarley³

et al. 2017

J microb biotech food sci

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Some bacteria produce antimicrobial chemicals in their immediate environments. These antimicrobial agents are enzymes, polypeptides or non-protein organic compounds. In this study, a bacterial isolate that produces antifungal chemical(s) was isolated from an over incubated nutrient agar plate that was exposed to air. The bacterium is aerobic, Gram positive bacilli; capsule and endospore producing. It ferments glucose and sucrose but not lactose, galactose, mannitol and sorbitol; it is citrate, indole, methyl red and Voges Prauskauer negative. Using agar gel diffusion technique, the cell-free culture supernatant resulting from centrifugation of a six day bacterial culture showed antifungal activity against filamentous fungi but not yeasts. Heating the cell-free supernatant in 90 ˚C water bath and digestion with different proteases had no negative impact on the antifungal activity. A segment of 16S rRNA gene of the bacterial isolate was amplified. The nucleotide sequence of the amplicon was used to identify the bacterium as being very similar to Bacillus amyloliquefaciens subsp. plantarum strain FZB42. Five bacterial peptides were isolated and identified from cell free supernatant of the bacterial culture using a suite of techniques, including flash chromatography, HPLC, NMR and mass spectrometry. One of the five peptides has been previously reported in literature to possess antifungal activity.

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“…The absence of a germination tube emerging from the surface of conidia indicated that bacterial compounds completely inhibited the spore germination in P. verrucosum, A. westerdijikae and F. oxysporum. These findings are in line with earlier studies [30,31], reporting similar spore germination inhibition by bacterial antifungal compounds. The ability of the QBC03 antagonistic compounds to maintain their antifungal activity, even after boiling for 30 min, is the most promising feature for their possible application in future.…”

Section: Discussionsupporting

confidence: 93%

In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius

Zeidan

Hassan

Al‐Thani

et al. 2019

Toxins

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Mycotoxins are secondary metabolites produced by certain filamentous fungi, causing human and animal health issues upon the ingestion of contaminated food and feed. Among the safest approaches to the control of mycotoxigenic fungi and mycotoxin detoxification is the application of microbial biocontrol agents. Burkholderia cepacia is known for producing metabolites active against a broad number of pathogenic fungi. In this study, the antifungal potential of a Qatari strain of Burkholderia cepacia (QBC03) was explored. QBC03 exhibited antifungal activity against a wide range of mycotoxigenic, as well as phytopathogenic, fungal genera and species. The QBC03 culture supernatant significantly inhibited the growth of Aspergillus carbonarius, Fusarium culmorum and Penicillium verrucosum in PDA medium, as well as A. carbonarius and P. verrucosum biomass in PDB medium. The QBC03 culture supernatant was found to dramatically reduce the synthesis of ochratoxin A (OTA) by A. carbonarius, in addition to inducing mycelia malformation. The antifungal activity of QBC03’s culture extract was retained following thermal treatment at 100 °C for 30 min. The findings of the present study advocate that QBC03 is a suitable biocontrol agent against toxigenic fungi, due to the inhibitory activity of its thermostable metabolites.

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Isolation and Characterization of Bacillus sp. Producing Broad-Spectrum Antibiotics Against Human and Plant Pathogenic Fungi

Cited by 12 publications

References 26 publications

Suppression of Fusarium Wilt in Watermelon by Bacillus amyloliquefaciens DHA55 through Extracellular Production of Antifungal Lipopeptides

Suppression of Fusarium Wilt in Watermelon by Bacillus amyloliquefaciens DHA55 through Extracellular Production of Antifungal Lipopeptides

Characterization and Isolation of Peptide Metabolites of an Antifungal Bacterial Isolate Identified as Bacillus Amyloliquefaciens Subspecies Plantarum Strain Fzb42

In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius

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