Isolation and characterization of bacteria from the rhizosphere of wheat

Abstract

In this study, we isolated bacteria from rhizosphere and endorhizophere of wheat crops of the central region of Argentina. The isolates were phenotypically characterized and the restriction patterns of 16S rDNA (ARDRA) using endonuclease AluI were analysed. Representative isolates were used to evaluate the effect of the inoculation on the growth of wheat under greenhouse conditions. The effects of plant growth-promoting bacteria on wheat plants were studied by evaluating shoot fresh and dry weights and root fresh and dry weights. One native strain increased the shoot and root dry biomass by 23% and 45% respectively. Other strains increased the shoot dry biomass. A 1.5 kb fragment of the 16S rRNA gene of one isolate was sequenced. This isolate showed high identity with different species of Pseudomonas.

Evaluation of Rhizospheric Bacteria from Ocimum sp. as Potential Pgpr

“…In our study, the two isolates showed activity of 3.90×10 -6 μmol α-keto butyrate/ mg protein/ h and 2.92×10 -6 μmol α-keto butyrate/mg protein/h by isolate UHI(II)7 and CHII(II)K7. Similar findings have been reported by other workers [47,48]. Plant growth by ACC deaminase producing Micrococcus sp.…”

Assessment of Rice Associated Bacterial Ability to Enhance Rice Seed Germination and Rice Growth Promotion

“…The final phylogenetic tree showed that the isolates were rather divers and from 40 isolates, 7 OTUs representing 31 isolates at the similarity of about 80% were obtained. Fingerprinting of 16S r-DNA has been used by many researchers to discriminate bacterial isolates genetically 7,12,45 . In such a similar practice, Loaces et al (2011), found 13 OTUs consisting of 55 isolates from 109 total isolates, whereas the rest showed unique restricted patterns indicating that the population density was quite variable .…”

The Role of Soil Beneficial Bacteria in Wheat Production: A Review

“…The use of bio-fertilizers with a good management can decrease the leaching loss of nitrate and phosphate from the agricultural land and improve the ground water quality [190]. Also, the use of PGPR with low-fertilizer rate is also an environment friendly step and would be a viable supplementary strategy for further increasing crop yields [71,78,191].…”