Isolation and Characterization of Bacteria from Sewage and Pond Water, Malda, India

Sarkar, Mihir; Chakraborty, Shiladitya; Kundu, Debtosh; Ghosh, Souradip; Khan, Ankita; Karmakar, Debankar; Ali, Md. Sarafat; Mandal, Shyamapada

doi:10.31080/asmi.2019.02.0394

Cited by 6 publications

(2 citation statements)

References 14 publications

(21 reference statements)

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“…The bacterial strain SRS1 was isolated from a clinical sample, and strain SRS4 was isolated from a sewage outlet sample from the same hospital. The antibiotic susceptibility test was performed according to Kirby-Bauer disc Diffusion Method as stated in (Sarkar et al, 2019), using the antibiotics amikacin, amoxiclav, aztreonam, ceftazidime, ciprofloxacin, cefepime, carbenicillin, colistin, gentamycin, meropenem, tazobactampiperacillin (Oxoid, UK). The sensitivity to antibiotics was determined according to CLSI guideline using the (Table 1) (CLSI, 2021).…”

Section: Isolation Of the Bacterial Strains And Antibiogrammentioning

confidence: 99%

Genomic Analysis to Elucidate the Antibiotic Resistance Mechanism of Extremely Drug-Resistant Pseudomonas Aeruginosa Strains Isolated From Bangladesh

Zahra

Jabeen

Uddin

et al. 2022

Bioresearch Communications

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Multidrug-resistant P. aeruginosa has potential to cause nosocomial infections. In this study, whole-genome sequencing was performed of two extremely drug-resistant novel strains SRS1 and SRS4 isolated from Bangladesh. The size of draft genome of SRS1 is 6.8 Mbp, and 7.0 Mbp for SRS4. In silico analysis predicted that the genome of SRS1 has 82 and SRS4 has 75 antibiotic-resistant genes (ARGs). Antibiogram results revealed that both SRS1 and SRS4 were resistant to multiple members of the antibiotic groups of β−lactam, quinolones, and aminoglycosides families. In addition, the genomes of both SRS1 and SRS4 were predicted to have multiple mobile elements like prophages and plasmids. Comparative genome analysis with wildtype PAO1 and another drug-resistant P. aeruginosa strain JNQH-PA57 revealed that SRS1 and SRS4 contain more antibiotic resistance genes like AAC (6´)-II, ANT (2´´)-Ia, ANT (3´´)-IIa, OXA-395, PME-1, qacEΔ1, tet(A), tet(D), VEB-9 than PAO1 and JNQH-PA57. This study shows the importance of the genomic study to understand the distribution of ARGs in Bangladeshi P. aeruginosa strains to demonstrate the mechanisms responsible for multi drug resistance. Bioresearch Commu. 9(1): 1208-1214, 2023 (January)

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Section: Isolation Of the Bacterial Strains And Antibiogrammentioning

confidence: 99%

Genomic Analysis to Elucidate the Antibiotic Resistance Mechanism of Extremely Drug-Resistant Pseudomonas Aeruginosa Strains Isolated From Bangladesh

Zahra

Jabeen

Uddin

et al. 2022

Bioresearch Communications

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“…Superbug have also reported to be found in the air environment of hospitals, livestock sector, and sewage treatment plants (Zhou et al, 2020). In the aquatic environment, superbugs can also be found due to the fact that in many areas, water bodies become the estuaries of domestic waste (Puspita et al, 2016), hospital waste (Sarkar et al, 2019), agricultural waste (Habibi et al, 2014), mining waste (Mulyadi et al, 2020), and industrial wastewater (Rahardjo & Prasetyaningsih, 2021). This causes adverse ecotoxicological effects (Smith et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genetic Identification of Superbugs from River Streams in State University of Malang

Sholekah

Listyorini

2023

JRBA

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Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.

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Determination of Trace and Heavy Metals in Selected Samples of Oregano (Origanum Vulgare L.) from Bosnia and Herzegovina

Behmen,

Mandal,

Dundović

et al. 2022

10th Central European Congress on Food

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Isolation and Characterization of Bacteria from Sewage and Pond Water, Malda, India

Cited by 6 publications

References 14 publications

Genomic Analysis to Elucidate the Antibiotic Resistance Mechanism of Extremely Drug-Resistant Pseudomonas Aeruginosa Strains Isolated From Bangladesh

Genomic Analysis to Elucidate the Antibiotic Resistance Mechanism of Extremely Drug-Resistant Pseudomonas Aeruginosa Strains Isolated From Bangladesh

Genetic Identification of Superbugs from River Streams in State University of Malang

Determination of Trace and Heavy Metals in Selected Samples of Oregano (Origanum Vulgare L.) from Bosnia and Herzegovina

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