Isolation and Characterization of Bacteria from Natural Hot Spring and Insights into the Thermophilic Cellulase Production

Sarangthem, Indira; Rajkumari, Lynda; Ngashangva, Ng; Nandeibam, Jusna; Yendrembam, Randhir B S

doi:10.1007/s00284-022-03168-x

Cited by 10 publications

(2 citation statements)

References 54 publications

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“…Mousa et al ( 2019 ) reported that, the optimum pH value was 7 at 35 °C for Achromobacter spanius . Sarangthem et al ( 2023 ) reported that, the optimum temperature and pH for cellulase activity were at 55 °C and pH 7.0 by B. velezensis . The enzyme is stable over a wide range of temperatures from 30 to 70 °C with maximum activity observed at 48 h of incubation.…”

Section: Discussionmentioning

confidence: 99%

In vivo application of potent probiotics for enhancing potato growth and controlling Ralstonia solanacearum and Fusarium oxysporum infections

Abdel-Moghies,

El-Sehrawy,

Zakaria

et al. 2024

Antonie van Leeuwenhoek

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Plant probiotics are live microbial cells or cultures that support plant growth and control plant pathogens through different mechanisms. They have various effects on plants, including plant growth promotion through the production of indole acetic acid (IAA), biological control activity (BCA), and production of cellulase enzymes, thus inducing systemic resistance and increasing the availability of mineral elements. The present work aimed to study the potential of Achromobacter marplatensis and Bacillus velezensis as plant probiotics for the field cultivation of potatoes. In vitro studies have demonstrated the ability of selected probiotics to produce IAA and cellulase, as well as antimicrobial activity against two plant pathogens that infect Solanum tuberosum as Fusarium oxysporum and Ralstonia solanacearum under different conditions at a broad range of different temperatures and pH values. In vivo study of the effects of the probiotics A. marplatensis and B. velezensis on S. tuberosum plants grown in sandy clay loamy soil was detected after cultivation for 90 days. Probiotic isolates A. marplatensis and B. velezensis were able to tolerate ultraviolet radiation (UV) exposure for up to two hours, the dose response curve exhibited that the D10 values of A. marplatensis and B. velezensis were 28 and 16 respectively. In the case of loading both probiotics with broth, the shoot dry weight was increased significantly from 28 in the control to 50 g, shoot length increased from 24 to 45.7 cm, branches numbers increased from 40 to 70 branch, leaves number increased from 99 to 130 leaf, root dry weight increased from 9.3 to 12.9 g, root length increased from 24 to 35.7 cm, tuber weight increased from 15 to 37.0 g and tubers number increased from 9 to 24.4 tuber, the rot percentage was reduced to 0%. The addition of both probiotic isolates, either broth or wheat grains load separately has enhanced all the growth parameters; however, better results and increased production were in favor of adding probiotics with broth more than wheat. On the other hand, both probiotics showed a remarkable protective effect against potato pathogens separately and reduced the negative impact of the infection using them together.

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Section: Discussionmentioning

confidence: 99%

In vivo application of potent probiotics for enhancing potato growth and controlling Ralstonia solanacearum and Fusarium oxysporum infections

Abdel-Moghies,

El-Sehrawy,

Zakaria

et al. 2024

Antonie van Leeuwenhoek

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“…The equation of the standard curve is y = 0.5515 × +0.0206 and the correlation coefficient is R 2 = 0.9996 (Supplementary Table 3). One unit (U) is defined as the amount of enzyme required to produce 1 µmol of glucose equivalent released per minute under the conditions described above (Li et al, 2020;Sarangthem et al, 2023).…”

Section: Identification Of Cellulase Production By Pathogenic Strains...mentioning

confidence: 99%

Isolation and identification of pathogens of Morchella sextelata bacterial disease

Zhu,

Ma,

Sun

et al. 2023

Front. Microbiol.

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Morel mushroom (Morchella spp.) is a rare edible and medicinal fungus distributed worldwide. It is highly desired by the majority of consumers. Bacterial diseases have been commonly observed during artificial cultivation of Morchella sextelata. Bacterial pathogens spread rapidly and cause a wide range of infections, severely affecting the yield and quality of M. sextelata. In this study, two strains of bacterial pathogens, named M-B and M-5, were isolated, cultured, and purified from the tissues of the infected M. sextelata. Koch’s postulates were used to determine the pathogenicity of bacteria affecting M. sextelata, and the pathogens were identified through morphological observation, physiological and biochemical analyses, and 16S rRNA gene sequence analysis. Subsequently, the effect of temperature on the growth of pathogenic bacteria, the inhibitory effect of the bacteria on M. sextelata on plates, and the changes in mycelial morphology of M. sextelata mycelium were analyzed when M. sextelata mycelium was double-cultured with pathogenic bacteria on plates. The results revealed that M-B was Pseudomonas chlororaphis subsp. aureofaciens and M-5 was Bacillus subtilis. Strain M-B started to multiply at 10–15°C, and strain M-5 started at 15–20°C. On the plates, the pathogenic bacteria also produced significant inhibition of M. sextelata mycelium, and the observation of mycelial morphology under the scanning electron microscopy revealed that the inhibited mycelium underwent obvious drying and crumpling, and the healthy mycelium were more plump. Thus, this study clarified the pathogens, optimal growth environment, and characteristics of M. sextelata bacterial diseases, thereby providing valuable basic data for the disease prevention and control of Morchella production.

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Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing

Le,

Zhang,

et al. 2024

Engineering Microbiology

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Isolation and Characterization of Bacteria from Natural Hot Spring and Insights into the Thermophilic Cellulase Production

Cited by 10 publications

References 54 publications

In vivo application of potent probiotics for enhancing potato growth and controlling Ralstonia solanacearum and Fusarium oxysporum infections

In vivo application of potent probiotics for enhancing potato growth and controlling Ralstonia solanacearum and Fusarium oxysporum infections

Isolation and identification of pathogens of Morchella sextelata bacterial disease

Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing

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