Isolation and characterization of bacterial endophytes of Curcuma longa L.

Abstract

Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis. All the endophytic strains utilized glucose, sucrose and yeast extract as a carbon source where as glycine, alanine, cystine and glutamine as nitrogen source. The strains were mostly sensitive to antibiotic chloramphenicol followed by erythromycin while resistant to polymixin B. The endophytic strains effectively inhibit the growth of Escherichia coli, Klebsiella pneumoniae and some of the fungal strain like Fusarium solani and Alterneria alternata. The strain ECL2 and ECL4 tolerated maximum 8 % of NaCl concentration where as strains ECL5 and ECL6 6 % in salinity tolerance.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0393-y) contains supplementary material, which is available to authorized users.

Enzymatic Activity of Endophytic Bacterial Isolates from Selected Mangrove Plants in Kenya

“…However, isolate MD12BGroot isolated from the root of Bruigeria gymnorhiza obtained from Mida creek and Gazi bay and isolate MA4XGleaf isolated from leaves of Xylocarpus granatum obtained from Mida creek did not show presence of amylases indicating the enzyme is not actively expressed. Similar studies on bacterial endophytes of Curcuma have shown the presence of amylases in the Bacillus species [26].…”

“…Endophytes are microbes that inhabit asymptomatically in the living tissues of plants without causing any substantive negative effect [1]. They colonize the plant tissue both inter-and intracellularly where they exhibit complex interactions with their host [2].…”

An Industry in Transition: The Chemical Industry and the Megatrends Driving Its Forthcoming Transformation

“…[35] In early 2018, the same company started to supply ashoe manufacturer based in San Francisco with ethylene vinyl acetate (EVA) produced from sugarcane-based ethanol. [36] As renewable chemicals companies grow in size and financial strength thanks to revenues from fine chemical and specialty polymer sales,t hey will be increasingly able to target commodity-type polymers starting from immensely abundant lignocellulosic biomass (731 million tonnes of rice straw alone), [37] rather than from vegetable oils or crops.…”

CULTURABLE ENDOPHYTIC BACTERIA FROM HALOTOLERANT Salvadora persica L.: SCREENING, ISOLATION AND PLANT GROWTH PROMOTING TRAITS

“…These strains previously reported as endophytes in different plant species like Bacillus aerius from Spharanthus indicus [22], Pantoea agglomerans from citrus [23], Serratia nematodiphila from Solanum nigrum [24], Enterobacter sp. from Curcuma longa L [25]. Many endophytic microorganisms have the potential to produce IAA.…”