Isolation and characterization of bacteriophages Salmonella spp.

Abstract

Against the background of reduced immunity of the body, the role of pathogenic and opportunistic microorganisms, which usually circulate in poultry farms in various associations, among the causative agents of poultry bacteriosis, is sharply increasing. Salmonella is one of the main bacteria affecting poultry and the most essential zoonotic pathogen causing foodborne illness. The work aimed to search and isolate lytic phages active in museum strains of Salmonella from the environment of poultry farms. The presence of lytic phages in museum strains of salmonella (Salmonella typhimurium 144 and Salmonella adobraco 1) was determined in the poultry houses samples according to Oliver and Grazia's standard method. It was established that the frequency of isolation of bacteriophages to the strain Salmonella typhimurium 144 from poultry farms was from 17.6 to 30.8 %, and the strain Salmonella adobraco 1 was 1.5 to 3.0 times less. In contrast, most often, lytic bacteriophages were isolated from sewage poultry houses. The size of negative colonies was larger in bacteriophages against Salmonella typhimurium 144 strain and was 2.8 ± 0.3 mm, which is 1.4 times larger, compared with colonies of bacteriophages lytic against Salmonella adobraco 1 strain. According to the degree of transparency, bacteriophages of Salmonella typhimurium 144 strain were primarily transparent. At the same time, the colonies of Salmonella adobraco 1 phages were translucent in most cases. Therefore, isolated bacteriophages for the Salmonella typhimurium 144 strain are better suited for developing a biological preparation for industrial poultry farming.