Isolation and Characterization of Biosurfactant Producing and Crude Oil Degrading Bacteria from Oil Contaminated Soils

Ebadi, Ali; Olamaee, M; Sima, Nayer Azam Khoshkholgh; Nasrabadi, Reza Ghorbani; Hashemi, Maryam

doi:10.1007/s40995-017-0162-8

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…Based on a gravimetric analysis, their efficiency to degrade crude oil in saline mineral broth varied from 33 to 39.2% ( Table 2 ). Details related to results of isolation and characterization of biosurfactant-producing and oil-degrading bacteria are reported in our previous work [14] . A gas chromatography-flame ionization based analysis of the crude oil degradation by each strain is given in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The chosen levels of crude oil were 10 and 30 g/kg, and the NaCl concentration was set to either 0, 150 or 300 mM. Salinity and contamination levels were chosen based on reports of literatures from contaminated sites of Iran [14] , [15] , [16] .…”

Section: Methodsmentioning

confidence: 99%

“…The strains were identified and differentiated from one another via 16S rRNA sequencing using the pair of universal primers 27F (5′AGA GTT TGA TCC TGG CTCAG3′) and 1429R (5′TAC GGY TAC CTT GTT ACG ACTT3′). An evaluation was conducted of each strain's capacity to produce biosurfactant and to degrade crude oil by culturing them in a saline medium which essentially as previously described by our group [14] . To construct the consortium, the strains were first cultured separately in aerobic tryptic soy broth at 30 °C for 24 h. The cells were harvested by centrifugation (10,000 g for 10 min) and resuspended in sterile 0.9% NaCl.…”

Section: Methodsmentioning

confidence: 99%

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Effective bioremediation of a petroleum-polluted saline soil by a surfactant-producing Pseudomonas aeruginosa consortium

Ebadi

Sima

Olamaee

et al. 2017

Journal of Advanced Research

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100

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effective bioremediation of a petroleum-polluted saline soil by a surfactant-producing Pseudomonas aeruginosa consortium

Ebadi

Sima

Olamaee

et al. 2017

Journal of Advanced Research

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100

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“…It was also demonstrated that branched alkanes as well as linear alkanes were degraded by the isolates. Based on 16S rDNA sequencing, all four isolates were identified as Pseudomonas aeruginosa [68]. A hydrocarbon-degrading, biosurfactant-producing, and plant-growth-promoting endophytic bacterium, Pseudomonas aeruginosa L10, was isolated from the roots of a reed, Phragmites australis, in the Yellow River Delta, Shandong, China.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Petroleum Hydrocarbons Degradation by Klebsiella pneumoniae and Pseudomonas aeruginosa

et al. 2018

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: The aim of this work was to develop bacterial communities to effectively degrade petroleum hydrocarbons (PHs). We investigated the biotic and abiotic contributors to differences in PHs degradation efficacy between two bacterial strains, Klebsiella pneumoniae (Kp) and Pseudomonas aeruginosa (Pa), screened out from the activated sludge of a petroleum refinery. We characterized the temporal variations in degradation efficacy for diesel and its five major constituents as a sole carbon source and identified more constituents they degraded. The growth characteristics, surface tension, hydrophobicity and emulsifiability of these two strains were measured. We further estimated the relationships between their degradation efficacy and all the biotic and abiotic factors. Results showed that the Pa strain had higher diesel degradation efficacy (58% on Day 14) and utilized more diesel constituents (86%) compared to Kp. Additionally, the growth of the Pa strain in diesel medium was faster than that of the Kp strain. The Pa strain had a lower surface tension and higher hydrophobicity and emulsifiability than Kp, while the surfactant produced by Pa was identified as rhamnolipids. Degradation of PHs was positively related to bacterial growth, hydrophobicity and emulsification but negatively related to surface tension. Overall, differences in degrading capacity for diesel constituents, relative growth rate, and biosurfactant production contributed to the variation in the PHs degradation efficacy of these two bacterial strains.

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“…In addition, the use of bio-friendly surfactants to remediate petroleum hydrocarbon contaminated soil has the advantages of good environmental benefits and low cost. It has broad development prospects and important application value in soil petroleum hydrocarbon pollution remediation [21,22].…”

Section: Introductionmentioning

confidence: 99%

Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

Liu

Zhu

et al. 2020

Environmental Engineering Research

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It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

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Isolation and Characterization of Biosurfactant Producing and Crude Oil Degrading Bacteria from Oil Contaminated Soils

Cited by 15 publications

References 31 publications

Effective bioremediation of a petroleum-polluted saline soil by a surfactant-producing Pseudomonas aeruginosa consortium

Effective bioremediation of a petroleum-polluted saline soil by a surfactant-producing Pseudomonas aeruginosa consortium

Comparison of Petroleum Hydrocarbons Degradation by Klebsiella pneumoniae and Pseudomonas aeruginosa

Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

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