Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina

Maidana, Silvina Soledad; Lomonaco, Patricia M; Combessies, G.; Craig, María Isabel; Diodati, Julian Angel; Rodriguez, D.; Parreño, Viviana; Zábal, Osvaldo; Konrad, J. L.; Crudelli, G.; Mauroy, Axel; Thiry, Étienne; Romera, Sonia Alejandra

doi:10.1186/1746-6148-8-83

Cited by 54 publications

(63 citation statements)

References 21 publications

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“…There are few reports on the circulation of RSV and PI3V in Mexican cattle, and there is no report on the isolation and/or sequencing of these viruses in small ruminants. Virologic isolation and physical-chemical characterizarion of PI3V in bovines has been reported in South America, but no phylogenetic studies were performed on these isolates [21]. PI3V strains have been isolated and characterized from beef cattle in the USA, and the isolation of this agent was reported in China, speculating that it could have derived from North America [47], however, our phylogenetic tree shows that Mexican strains do not come from USA and they are in a different clade to that reported by Wen et al [47].…”

Section: Resultsmentioning

confidence: 99%

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

et al. 2017

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This is a first report in Mexico of the presence of antibodies against respiratory syncytial virus (RSV) and parainfluenza-3 virus in Mexican sheep in different productive stages. We determine the association of serological positivity with age and production system, and obtain molecular evidence of infection by both virus. RSV prevalence in adult sheep was 47% (49/105) at the tropic and 64% (63/99) at the uplands. A significant difference in RSV seropositivity between animals from the tropic and the uplands was observed (P \ 0.05). Seropositivity correlated with production system (P = 0.003, OR = 2.042), with a risk of showing antibodies was 2.042 times higher in sheep under an extensive production system. A significant difference in PI3V seropositivity between animals from either provenance (P = 0.017, OR = 0.475) were also found, with a risk of showing antibodies 0.475 times higher in sheep under an extensive production system. Genetic material from RSV and PI3V was identified by RT-PCR in nasal swab samples from clinically healthy lambs and confirmed by sequencing and phylogenetic analysis. Serological results show that sheep are susceptible to infection by both viruses, and molecular results suggest that the identified antibodies are result of natural infections and reinfections.

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Section: Resultsmentioning

confidence: 99%

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

et al. 2017

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“…Thus the authors suggested that those strains should be considered as genotype C BPIV3 strains (BPIV3c) [11]. A similar strategy for detection and molecular characterization of BPIV3 was reported by a number of authors [1,3,4,6,11,15,16].…”

Section: Discussionmentioning

confidence: 99%

“…This is a multifunctional molecule with three different and crucial activities for cell infection: receptor binding activity, neuraminidase activity and fusion promotion activity [5]. Three genotypes: A (BPIV3a), B (BPIV3b) and C (BPIV3c) have been described, based on genetic and phylogenetic analysis [6]. Previously, the complete genome analysis of the BPIV3 isolates from Australia and America indicated that their viral strains were classifi ed into two distinct genotypes, BPIV3 genotype A and BPIV3 genotype B.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

et al. 2016

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The presence of bovine parainfl uenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confi rmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specifi c for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.

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“…After viral nucleic acid extraction process, nucleic acids, including RNA, the bovine respiratory syncytial virus and parainfl uenza-3, were converted to complementary DNA using RevertAid First Strand cDNA Synthesis Kit (Cat No: K1622, Thermo Scientifi c). The positivities for the viral agents of BRD were determined by the polymerase chain reaction (PCR) process [19][20][21].…”

Section: Analysis Of Virusesmentioning

confidence: 99%

Cardiac Troponin-I Concentration In Weaned Calves With Bovine Respiratory Disease

et al. 2015

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The aim of this study was to evaluate the serum cardiac troponin I (cTnI) concentrations in weaned calves with moderate and severe bovine respiratory disease. Eighteen healthy weaned calves (group I), 18 weaned calves with moderate bovine respiratory disease (group II) and 18 weaned calves with severe bovine respiratory disease (group III) were included in the study. Clinical signs and severity of respiratory disease according to clinical index scores were determined. Blood cell counts and cardiac biomarkers, such as serum cTnI concentrations and creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase (LDH) activities were determined in all groups. Temperature and respiratory and pulse rates were signifi cantly increased in calves with moderate and severe bovine respiratory disease compared to healthy calves (P < 0.05). Serum cTnI concentrations and LDH activities were signifi cantly increased in the calves with severe bovine respiratory disease compared to healthy calves and calves with moderate bovine respiratory disease (P < 0.05). A signifi cant increase in white blood cell counts in calves with moderate and severe bovine respiratory disease and lymphopenia and neutrophilia in calves with severe bovine respiratory disease were also found. The study suggests that severe bovine respiratory disease causes increased serum cTnI concentrations in weaned calves.

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Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina

Cited by 54 publications

References 21 publications

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

Cardiac Troponin-I Concentration In Weaned Calves With Bovine Respiratory Disease

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