Isolation and Characterization of Brucella spp., Low-Density Polyethylene (LDPE) Plastic Degrading Bacteria in Al-Ahsa Region, Saudi Arabia

Alamer, Narjes J.; Aldayel, Munirah F.; Khalifa, Ashraf

doi:10.3390/app13074629

Cited by 6 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have claimed that weight loss is the initial phase of depolymerization and biodegradation of plastics (Alamer et al 2023). This method can also be used to determine the amounts of CO 2 or CH 4 produced during the degradation of plastics.…”

Section: Weight Loss Measurementmentioning

confidence: 99%

Characterization of polystyrene-degrading bacteria isolated from plastic processing plants

Torkar,

Šunta,

Fink

et al. 2024

Preprint

View full text Add to dashboard Cite

In this study, the occurrence of the most common types of bacteria and fungi on the surfaces of production lines, raw materials and finished products in plastics processing plants was investigated. In addition, the ability of selected bacterial strains to form biofilms and to degrade PS surfaces was determined. The surfaces examined mainly contained aerobic mesophilic bacteria (42.0% of the samples), followed by moulds (30.0%) and representatives of the genera Bacillus (28.3%) and Staphylococcus (19.9%). Growth on the solid medium with polystyrene was confirmed in 63 (63.4%) of the 96 selected strains, but only 3 (3.0%) of them were able to form a clear zone around the colonies. The highest weight loss of polystyrene was observed in the presence of Bacillus strains. The degree of degradation after 30 and 60 days ranged from 0.12–0.78% and from 0.21–2.15%, respectively. The number of living cells in biofilms on surfaces was lowest for the Gram-negative strains Burkholderia and Moraxella and highest for most Bacillus and some Micrococcus strains. Fourier transform infrared spectroscopy revealed differences in the ratios of vibrational bands after exposure to bacterial strains, although no oxygen-containing functional groups were observed on the surface of polystyrene samples. The average contact angle values of polystyrene surfaces exposed to the tested bacterial strains decreased from 80.55 ± 0.39° to 73.64 ± 0.45° after 60 days of incubation. Some isolated bacterial strains from plastic production plants show the potential for more intensive degradation of such materials, which could have an impact on the quality and shelf life of plastic products.

show abstract

Section: Weight Loss Measurementmentioning

confidence: 99%

Characterization of polystyrene-degrading bacteria isolated from plastic processing plants

Torkar,

Šunta,

Fink

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Plastic waste in macro and micro conditions can cause negative impacts if consumed by marine organisms through the food chain (Emmanuel-Akerele and Akinyemi, 2022; Fawcett et al, 2021). Bacteria have the ability to break down plastic naturally, which is called biodegradation (Alamer et al, 2023;Skleničková et al, 2022). It is necessary to use plastic waste degrading bacteria as a solution.…”

Section: Introductionmentioning

confidence: 99%

16S rRNA Gen Analysis of Plastic Destruction Bacteries, South Sumatra, Indonesia

Meiyerani,

Melki,

Aryawati

et al. 2024

J. Ecol. Eng.

View full text Add to dashboard Cite

Rivers are the main route for plastic entering the ocean, including the Musi River Estuary. Characteristics of bacteria that are able to degrade plastic waste through polymerase enzymes. The aim of this research is to determine the ability of bacterial isolates to degrade plastic and identify the types of bacteria that degrade plastic waste. This study used plastic bottles, nylon nets, and snack wrappers as objects for degradation measurement. Identification analysis of the 16S rRNA gene using universal PCR primers for bacteria in the form of forward primer 63f (5'-CAG GCC TAA CAC ATG CAA GTC-3') and reverse primer 1387r (5'-GGG CGG WGT GTA CAA GGC-3'). The type of bacteria with the highest percentage of degradation over 20 days, amounting to 7.75%, was Bacillus amyloliquefaciens. Identification of types of plastic degrading bacteria using 16S rRNA gene analysis showed 11 bacteria with 8 types including Staphylococcus hominis, Pseudomonas aeruginosa, Acinetobacter sp., Acinetobacter baumannii, Acinetobacter variabilis, Shewanella sp., Micrococcus luteus, and Bacillus amyloliquefaciens. The percentage of plastic degradation by bacteria is relatively small, so it is best to look for times where there is potential for bacterial growth.

show abstract

Microbial Degradation of Polyester Microfibers Using Indigenously Isolated Bacterial Strain Exiguobacterium Sp.

Mishra,

Dash,

Das

2024

CLEAN Soil Air Water

View full text Add to dashboard Cite

Synthetic microfibers are emerging environmental microplastic pollutants released from different industrial and domestic sources. The present investigation describes the isolation of potential bacterial strains from microplastic‐contaminated sites of Bhubaneswar city of Odisha, India. Four morphologically distinct bacterial strains were isolated using 2% polyethylene glycol (PEG) supplemented nutrient agar (NA) medium and were screened for their polymer tolerance ability by growing them on 2%–8% PEG. A single microorganism capable of growing on 8% PEG was selected for biodegradation experiment. Through 16S rRNA sequencing, the selected bacterial strain was identified as Exiguobacterium sp. with gene bank accession number ON318396. The microbial strain's microfiber biodegradation ability was assessed in a laboratory setting over a period of 28 ± 2 days, utilizing optimized conditions with an initial pH of 7, 2 mL inoculum volume, an incubation temperature of 30°C ± 2°C, and 150 rpm, using 2 g of polyester microfiber. In optimum conditions, the weight loss of the treated sample with the selected microbial strain was 19.2%. The polyester degradation was confirmed through scanning electron microscopic images viewing the degradation of the polyester microfiber surfaces. Variation in functional groups confirmed through Fourier transform infrared spectrophotometry. Detection of carbonyl (C═O) group stretching band at 1711 cm−1 through ATR‐FTIR analysis in the treated sample confirmed the polymer biodegradation. The potential isolate can efficiently degrade polyester and, in the future, can be employed as a promising solution for the sustainable treatment of synthetic microfiber pollution.

show abstract

Isolation and Characterization of Brucella spp., Low-Density Polyethylene (LDPE) Plastic Degrading Bacteria in Al-Ahsa Region, Saudi Arabia

Cited by 6 publications

References 57 publications

Characterization of polystyrene-degrading bacteria isolated from plastic processing plants

Characterization of polystyrene-degrading bacteria isolated from plastic processing plants

16S rRNA Gen Analysis of Plastic Destruction Bacteries, South Sumatra, Indonesia

Microbial Degradation of Polyester Microfibers Using Indigenously Isolated Bacterial Strain Exiguobacterium Sp.

Contact Info

Product

Resources

About