Isolation and characterization of catalase isoforms from the mango stone weevil, Sternochetus mangiferae

Jayanthi, P. D. Kamala; Aurade, Ravindra M.; Ayyasamy, Arthikirubha; Kempraj, Vivek

doi:10.1016/j.aspen.2016.01.007

Cited by 7 publications

(5 citation statements)

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“…Among antioxidant enzymes, catalase (CAT) plays a crucial role in protecting the host from ROS damage. [21][22][23][24] The whitefly Bemisia tabaci (Gennadius) is one of the most devastating crop pests, causing serious crop yield reductions by transmitting plant viruses, feeding on phloem sap, and excreting honeydew. [25][26][27] B. tabaci is a species complex of at least 30 cryptic species, 28 among which the Mediterranean (MED) is highly invasive and has become distributed worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Silencing of catalase reduces unfavorable low‐temperature tolerance capacity in whiteflies

Ning,

Liang,

et al. 2024

Pest Management Science

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BACKGROUNDTemperature is a primary factor that determines the eco‐geographical distribution and population development of invasive insects. Temperature stress leads to various negative effects, including excess reactive oxygen species (ROS), and catalase (CAT) is a key enzyme against ROS in the antioxidant pathway. The whitefly Bemisia tabaci MED is a typical invasive pest that causes damage worldwide. Our previous studies have shown that CAT promotes whitefly adaptation to high temperature by eliminating ROS. However, the mechanism underlying the low‐temperature adaptation of whiteflies is still unknown.RESULTSIn this study, we investigated the role of CAT in the low‐temperature tolerance of B. tabaci MED by analyzing its survival rate, reproduction, and ROS levels at 25 °C (as a control, suitable temperature), 20 °C (moderately decreased temperature), and 4 °C (severely decreased temperature). Silencing of BtCAT1, BtCAT2, or BtCAT3 reduced the viability of whiteflies under a short‐term severely decreased temperature (4 °C), which manifested as decreases in survival and fecundity accompanied by significant increases in ROS levels. Moreover, even at a moderately decreased temperature (20 °C), silencing of BtCAT1 led to high ROS levels and low survival rates in adults.CONCLUSIONSilencing of BtCATs significantly increased the sensitivity of B. tabaci MED to low temperatures. BtCAT1 is likely more essential than other BtCATs for low‐temperature tolerance in whiteflies.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

“…Antioxidant enzyme systems are vital for insects to defend themselves against ROS. Among antioxidant enzymes, catalase (CAT) plays a crucial role in protecting the host from ROS damage 21–24 …”

Section: Introductionmentioning

confidence: 99%

Silencing of catalase reduces unfavorable low‐temperature tolerance capacity in whiteflies

Ning,

Liang,

et al. 2024

Pest Management Science

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“…En esta etapa cabe destacar que se realizaron pruebas de elución de la Catalasa con la disolución utilizada con el monolito cerámico, NaSCN 0.2 M en PBS pH 7.4 [175]. Sin embargo, en este monolito se observa que el rendimiento de elución no supera el 68%, y que la Catalasa obtenida pierde su actividad, seguramente porque la presencia del ion tiocianato envenena la enzima [177][178][179], lo que hace que esta disolución no sea adecuada para esta aplicación.…”

Section: Elución Específicaunclassified

“…Además, en este tipo de cromatografía de afinidad, los agentes competitivos más utilizados en la elución son el imidazol y el tiocianato de sodio [65,[187][188][189]. Sin embargo este último provoca el envenenamiento de la Catalasa [177][178][179], por lo que no es conveniente su utilización cuando se recupera con el fin de utilizarla posteriormente.…”

Section: Estudios De Elución De La Enzima Catalasaunclassified

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Desarrollo y validación de soportes macroporosos para la separación de proteínas mediante cromatografía IMAC

Pérez¹

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En primer lugar, quiero agradecer toda la ayuda y entrega de mis directores de la presente tesis doctoral, los Catedráticos Miguel Ángel Galán Serrano y Eva M.Martín del Valle. Muchas gracias por vuestra confianza y por ser una fuente continua de aprendizaje.Al Departamento de Ingeniería Química que me ha ayudado desde el primer momento, en especial Julián, Manolo, Belén (secretaría), María, Belén y Nekane.Gracias por tenderme la mano siempre que he necesitado ayuda.A todos mis compañeros de laboratorio que han hecho más llevadero este camino, en especial a

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Distribution, purification and characterization of a monofunctional catalase from Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Ghanem

Mohamed

Abdelaziz

2020

Biocatalysis and Agricultural Biotechnology

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Isolation and characterization of catalase isoforms from the mango stone weevil, Sternochetus mangiferae

Cited by 7 publications

References 34 publications

Silencing of catalase reduces unfavorable low‐temperature tolerance capacity in whiteflies

Silencing of catalase reduces unfavorable low‐temperature tolerance capacity in whiteflies

Desarrollo y validación de soportes macroporosos para la separación de proteínas mediante cromatografía IMAC

Distribution, purification and characterization of a monofunctional catalase from Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

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