Isolation and characterization of CCoAOMT in interspecific hybrid of Acacia auriculiformis x Acacia mangium - a key gene in lignin biosynthesis

Pang, Shek Ling; Ong, Socheath; Lee, H.H.; Zamri, Z.; Kandasamy, Kathiresan; Choong, Chee Yen; Wickneswari, R.

doi:10.4238/2014.september.5.7

Cited by 18 publications

(8 citation statements)

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“…High levels of CCOMT expression is associated with xylem development in woody plants (Shi et al, 2010;Yang et al, 2014). At present, many studies have used genetic engineering to suppress CCOMT expression to downregulate lignin (Wagner et al, 2011;Li et al, 2013;Pang et al, 2014;Zhang et al, 2014). In the present study, two CCOMT genes (PbCCOMT1 and PbCCOMT3) involved in lignin biosynthesis have been identified.…”

Section: Discussionmentioning

confidence: 85%

“…Suppression of CCOMT expression in maize showed that the lignin content was decreased and the ratio of S/G lignin units was increased . Research showed that heterologously expressed anti-AcCCOMT increased the levels of S units, G units, and total lignin in tobacco (Pang et al, 2014). The results of previous studies have shown that PbCCOMT genes are expressed at high levels during the early stages of fruit development, which might promote the accumulation of pear stone cells (Wu et al, 2012).…”

Section: Introductionmentioning

confidence: 98%

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Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. With high nutritional value in its fruits, Dangshan Su pear has been widely cultivated in China. The stone cell content in fruits is a key factor affecting fruit quality in pear, and the formation of stone cells has been associated with lignin biosynthesis. O-Methyltransferase (OMT) is a key enzyme involved in lignin metabolism within the phenylpropanoid pathway. Here, we screened 26 OMT genes from the Pyrus bretschneideri cv. Dangshan Su genome using the DNATOOLs software. To characterize the OMT gene family in pear, gene structure, chromosomal localization, and conserved motifs of PbOMTs were analyzed. PbOMTs were divided into two categories, type I (designated PbCCOMTs) and type II (designated PbCOMTs), indicating the differentiation of function during evolution. Based on the analysis of multiple sequence alignment, cis-element prediction, and phylogenetic relationships, two candidate genes, PbCCOMT1 and PbCCOMT3, were selected for the analysis of temporal and spatial gene expression in pear. The promoter regions of both PbCCOMT1 and PbCCOMT3 contain regulatory motifs for lignin synthesis. Moreover, the two genes show high similarity and close phylogenetic relationships with CCOMTs in other species. Expression analysis showed that transcript levels of two PbCCOMTs were positively associated with the contents of both stone cells and lignin during the development of pear fruit. These results suggest that PbCCOMT1 and PbCCOMT3 are closely associated with lignin biosynthesis. These findings will help clarify the function of PbOMTs in lignin metabolism and to elucidate the mechanisms underlying stone cell formation in pear.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 98%

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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“…Different approaches used to downregulate CCoAOMT, including antisense RNA, RNA interference, or gene knockout, resulted in decreased lignin content in plants, which was associated with collapsed vascular bundles, lower height, lower biomass and developmental retardation (Do et al 2007 ). Plants with downregulated CCoAOMT genes also exhibit modified lignin subunit composition due to disturbances in syringyl and guaiacyl monolignol synthesis (Li et al 2013 ; Meyermans et al 2000 ; Pang et al 2014 ; Pinçon et al 2001 ; Toraman et al 2016 ; Zhong et al 1998 ). CCoAOMT overexpression produces the opposite effects, including increased lignin content, plant height and silique length (Zhang et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Sorghum CCoAOMT and CCoAOMT-like gene evolution, structure, expression and the role of conserved amino acids in protein activity

et al. 2018

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Sorghum is a crop plant that is grown for seeds, sucrose, forage and biofuel production. In all these applications, lignin is a superfluous component that decreases the efficiency of technological processes. Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) is an enzyme involved in monolignol synthesis that affects the efficiency of lignification and lignin composition. The sorghum genome harbors one CCoAOMT gene and six closely related CCoAOMT-like genes. The structures of four sorghum CCoAOMT-like enzymes suggest that these proteins might methylate caffeoyl coenzyme A and contribute to monolignol synthesis. In this study, two sorghum genes, CCoAOMT and one CCoAOMT-like, were found to be highly expressed in leaves, stems and immature seeds. The promoters of these genes possess clusters of transcription factor-binding sites specific for lignification, and this suggests that they are important for lignification. Phylogenetic analysis revealed that one sorghum CCoAOMT-like enzyme is closely related to ancestral cyanobacterial CCoAOMT-like proteins. The remaining CCoAOMT-like enzymes, including the one highly expressed in the leaves and stem, are closely related to CCoAOMT. Genes from these two groups possess different, evolutionarily conserved gene structures. The structure of the sorghum CCoAOMT-like protein from the ancestral clade was modeled and differences between enzymes from the two clades were analyzed. These results facilitate a better understanding of the evolution of genes involved in lignification, and provide valuable data for sorghum improvement through traditional breeding or molecular genetic techniques. The findings suggest that CCoAOMT-like genes might be recruited in lignification and raise questions of the frequency of such functional shifts.Electronic supplementary materialThe online version of this article (10.1007/s00438-018-1441-6) contains supplementary material, which is available to authorized users.

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“…Lignin in stem cell walls of PAL -knockdown plants in Brachypodium distachyon is reduced by approximately 43% 33 . Downregulation of C4H in hybrid eucalyptus ( Eucalyptus urophylla × Eucalyptus grandis ) 34 , 4CL in hybrid poplar ( Populus tremula × Populus alba ) 35 , CCR in birch ( Betula platyphylla × Betula pendula ) 36 , CAD in Arabidopsis thaliana 37 , and a CCoAOMT homolog in tobacco ( Nicotiana tabacum ) 38 leads to varying degrees of reduction in lignin content compared to controls. The suppression of these genes also affects plant development, especially the repression of CCR , which reduces the thickness of secondary walls.…”

Section: Discussionmentioning

confidence: 99%

EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis

et al. 2019

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The mechanical strength of inflorescence stems is an important trait in cut flowers. Calcium ions (Ca 2+ ) play a pivotal role in maintaining stem strength, but little is known about the underlying molecular mechanisms. In this study, we treated herbaceous peony ( Paeonia lactiflora Pall.) with ethyl glycol tetraacetic acid (EGTA), an effective Ca 2+ chelator, and used morphology indicators, spectroscopic analysis, histochemical staining, electron microscopy, and proteomic techniques to investigate the role of Ca 2+ in inflorescence stem mechanical strength. The EGTA treatment reduced the mechanical strength of inflorescence stems, triggered the loss of Ca 2+ from cell walls, and reduced lignin in thickened secondary walls in xylem cells as determined by spectroscopic analysis and histochemical staining. Electron microscopy showed that the EGTA treatment also resulted in significantly fewer xylem cell layers with thickened secondary walls as well as in reducing the thickness of these secondary walls. The proteomic analysis showed 1065 differentially expressed proteins (DEPs) at the full-flowering stage (S4). By overlapping the Kyoto encyclopedia of genes and genomes (KEGG) and gene ontology (GO) analysis results, we identified 43 DEPs involved in signal transduction, transport, energy metabolism, carbohydrate metabolism, and secondary metabolite biosynthesis. Using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, we showed that EGTA treatment inhibited Ca 2+ sensors and secondary wall biosynthesis-related genes. Our findings revealed that EGTA treatment reduced the inflorescence stem mechanical strength by reducing lignin deposition in xylem cells through altering the expression of genes involved in Ca 2+ binding and secondary wall biosynthesis.

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Isolation and characterization of CCoAOMT in interspecific hybrid of Acacia auriculiformis x Acacia mangium - a key gene in lignin biosynthesis

Cited by 18 publications

References 49 publications

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Sorghum CCoAOMT and CCoAOMT-like gene evolution, structure, expression and the role of conserved amino acids in protein activity

EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis

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