Isolation and characterization of cDNA clones for plant cyclins.

Hata, Shingo; Kouchi, Hiroshi; Suzuka, Iwao; Ishii, Takahiro

doi:10.1002/j.1460-2075.1991.tb07811.x

Cited by 144 publications

(99 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The second highest similarity is to the alfalfa cyclin cycMs2 (Hirt et aL, 1992), with amino acid identities of 69.6 and 68.4% for cycOsl and cycOs2, respectively. Homologies to cyclins from Arabidopsis (Hemerly et al, 1992) and soybean (Hata et aL, 1991) (Minshull eta/., 1990;Hunt, 1987, 1989), is FMRRFLK in both cycOsl and cycOs2. Both clones are too short at the 5' end to include a mitotic destruction box.…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of two highly conserved regions in the 'cyclin box' of plant cyclins (Hata et aL, 1991;Hemerly et aL, 1992;Hirt et aL, 1992;Renaudin et aL, 1994) two degenerate primers were derived.…”

Section: Isolation Of Rice Cyclin Cdna Clonesmentioning

confidence: 99%

“…This indicates different functions for the products of these two genes in regulating the cell cycle in rice (Hashimoto et al, 1992). cDNA clones encoding plant cyclin homologs have been isolated from carrot and soybean (Hata et al, 1991), Arabidopsis (Hemerly et al, 1992), alfalfa (Hirt et al, 1992) and maize (Renaudin et al, 1994). Because plant cyclins show sequence homologies to both A-and B-type cyclins, an unequivocal assignment of their biological function within the cell cycle cannot be made on the basis of sequence information alone.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes

Sauter

Mekhedov

Kende³

1995

The Plant Journal

137

View full text Add to dashboard Cite

SummaryPartial submergence or treatment with either ethylene or gibberellin (GA) promotes rapid internodal growth in deepweter rice (Oryza sativa L.). Earlier work has shown that GA is the immediate hormonal signal for this growth response, which involves induction of the cell cycle at the G2/M phase transition and subsequent enhancement in the rate of DNA synthesis. In all eukaryotes, onset of mitosis is regulated by the p34 cdca/c°cas protein kinase, whose activity is assayed by in vitro phosphorylation of histone H1. It was found that GA enhanced the activity of p34~'z/coCn-like histone H1 kinase in the intercalary meristem of rice internodes. The enzyme activity showed a sharp peak that correlated with a decrease in the population of cells in the G2 phase during the first 4 h of GA treatment but not with changes in DNA synthesis. The level of histone H1 kinase activity increased again when cell division activity in the intercalary meristem is known to be high. The expression of two cdc2 homologs was examined. The mRNA level of one of these, cdc2Os-2, was increased after 1 h of GA treatment, whereas the mRNA level of the other, cdc2Os-1, was not affected. Two cDNAs, cycOsl and cycOs2, which show high homology to cyclin cDNAs, were cloned from rice. They share 75.1% sequence identity at the amino acid level, and both of them are encoded by mRNAs of 1.6 kb. Expression of the two corresponding cyclin genes was enhanced by GA, and the time course of the induction was compatible with a role for both cyclins in regulating the G2/M phase transition. The cyclins were expressed in the intercalary meristem and the elongation zone of the internode, but the GAinduced increase in transcript levels was restricted to the meristem only. The results support the hypothesis that induction of mitosis by GA is brought about by increased p34 cdc2/coc2s protein kinaee activity, which may be the result of transcriptional activation of the cdc2Os-2, cycOsl and cycOs2 genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Rice Cyclin Cdna Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes

Sauter

Mekhedov

Kende³

1995

The Plant Journal

137

View full text Add to dashboard Cite

show abstract

“…This strategy was successful for isolating theArabidopsis 3-hydroxy-3-methylglutaryl-CoA reductase gene (21,22). The mouse squalene synthase cDNA (11) was used as a probe, because we had found that plant cell-cycle-related genes were more similar to mammalian homologs than to yeast homologs (23)(24)(25)(26).…”

mentioning

confidence: 99%

Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase.

Nakashima

Inoue²,

Oka³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

We have isolated and characterized two overlapping cDNA clones for Arabidopsis thaliana squalene synthase. Their nucleotide sequences contained an open reading frame for a 410-amino acid polypeptide (calculated molecular mass, 47 kDa). The deduced amino acid sequence of the Arabidopsis polypeptide was significantly homologous (42-44% identical) to the sequences of known squalene synthases of several species, from yeast to man, but it was much less homologous to that of tomato phytoene synthase. To express the Arabidopsis enzyme in Escherichia coli, the entire coding region was subcloned into an expression vector. A cell-free extract of E. coli transformed with the recombinant plasmid, in the presence of NADPH and Mg2+, efficiently converted

show abstract

“…Poly (A)-rich RNA was isolated from a mixture of staged embryos (globular stage/heart stage/torpedo stage = 1 :2:4, fresh mass). The construction of a AZAPII cDNA library has already been described [15]. Genomic DNA was prepared [17] from a cell line, named IM-1, which was obtained from the above suspen-…”

mentioning

confidence: 99%

Identification of carrot cDNA clones encoding a second putative proliferating cell‐nuclear antigen, DNA polymerase δ auxiliary protein

Hata

Kouchi

Tanaka

et al. 1992

European Journal of Biochemistry

View full text Add to dashboard Cite

The proliferating cell-nuclear antigen (PCNA) plays a key role in the control of eukaryotic DNA replication. We have isolated two cross-hybridizing groups of cDNA encoding carrot homologs of PCNA. Sequence analysis and Southern-blot experiments showed that the cDNA were derived from two distinct genes. One corresponded to the typical PCNA, which is known to be highly conserved in eukaryotes from yeast to man; its mRNA is 1.2 kb in size and the calculated molecular mass of the protein is 29 kDa. The other encoded a larger PCNA homolog which has not previously been reported; the mRNA is 1.5 kb in size, the N-terminal three quarters (calculated molecular mass, 29 kDa) of the protein product is 88% identical at the amino acid level to the typical PCNA, but the protein has an extra C-terminal domain of 11 kDa. Both PCNA homologs were apparently coexpressed concomitant with somatic embryogenesis. The mRNA level of the novel homolog is 10 -20% that of the typical PCNA in the embryos. The presence of the second putative PCNA may provide new insight into studies on the mechanism of DNA replication in eukaryotes.The proliferating cell-nuclear antigen (PCNA) was originally discovered as a nuclear antigen in human proliferative cells [I]. Subsequently, it was identified as an auxiliary protein for DNA polymerase 6 [2, 31. PCNA plays an important role in eukaryotic DNA replication by increasing the processibility of the polymerase 6 during elongation of the leading strand [4-61. In addition, PCNA is a highly conserved protein in widely divergent species [7]. Previously, we showed that PCNA is also present in higher plants [8, 91 and that its expression is localized to the proliferating organs [lo]. So far, it has been believed that each organism contains only one molecular species of PCNA.Somatic embryogenesis in cultured carrot cells is an attractive system for investigating the mechanism of plant cell differentiation [Ill. A number of studies on the gene-expression program of somatic embryogenesis have been carried out and several genes were reported to be induced in the process [12-141. In those studies, however, the structures and functions of the genes remained unclear. We have recently Correspondence to S. Hata, Faculty of Science, Himeji Institute of Ahhrc4ution. PCNA, proliferating cell-nuclear antigen. Enzymes. DNA polymerase 6, modified T7 DNA polymerase (EC 2.7.7.7); restriction enzymes BcoRI, EcoT141, HindlII, PstI, XbuI (EC 3.1.21.4).Nore. The novel nucleotide sequence data published here have been deposited in the EMBL sequence data bank and are available under the accession numbers X62976 and X62977.Technology, Kamigori, Ako-gun, Hyogo, demonstrated that a mitotic cyclin gene is expressed concomitant with the embryogenesis [15].We undertook the present investigation to determine whether or not somatic embryogenesis occurs concomitant with the induction of PCNA expression. As a first step, we isolated cDNA for carrot PCNA using PCNA clones of other plants as probes. Unexpectedly, however, the cDNA formed two c...

show abstract

Isolation and characterization of cDNA clones for plant cyclins.

Cited by 144 publications

References 45 publications

Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes

Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes

Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase.

Identification of carrot cDNA clones encoding a second putative proliferating cell‐nuclear antigen, DNA polymerase δ auxiliary protein

Contact Info

Product

Resources

About