Isolation and Characterization of Cellulolytic Bacteria Diversity in Peatland Ecosystem and Their Cellulolytic Activities

Abstract

Peatlands are terrestrial wetland ecosystems formed from piles of organic matter that decompose into organic deposits. Peat soil has a high potential to produce cellulose which, can be reused by cellulolytic bacteria. This study aims to find out the potential strain of cellulolytic bacteria isolated from peatland ecosystems. The method used was experimental, sequentially, the stages are isolation and screening for cellulolytic bacteria, quantitative testing of cellulolytic activity, characterizing the morphology and physiology of bacteria, and the identification of bacteria based on Bergey’s Manual of Determinative Bacteriology. The screening results obtained seven isolates of cellulolytic bacteria capable of hydrolysed cellulose on 1% Carboxy Methyl Cellulose (CMC) Agar Medium, namely SPS1, SPS2, SPS 3, SDG1, SDG 2, SPW1, and SPW4. Three of seven isolates obtained the highest cellulolytic index sequentially, namely SPS2 of 2.82, SPS3 of 2.65, and SDG1 of 2.47. The cellulolytic activity was indicated by the value of a halo zone around the colonies on 1 % CMC medium after being dripped with Congo red. The halo zone is an early indication to determine the ability of bacteria to decompose cellulose. Based on Bergey’s Manual of Determinative Bacteriology showed that the three isolates had the same characteristics as the genus Bacillus, Lactobacillus and Corynebacterium.

Characterization of Microbial Decay and Microbial Communities in Waterlogged Archaeological Rosewood (Dalbergia Species)

“…However, except for Diaphorobacter [60], whether these detected bacteria have the potential to degrade lignin remains unclear. Similar to ligninolytic bacteria, many cellulolytic bacteria have been isolated from different sources, including pulp, soil, and various animals [63][64][65][66]. Compared with previous studies, no major bacterial genera detected in waterlogged archaeological rosewood could degrade cellulose in our study.…”

A Bacillus velezensis strain shows antimicrobial activity against soilborne and foliar fungi and oomycetes

“…Lipase activity was tested using tributyrin agar (TA), which is composed of 12 g agar, 10 mL glyceryl tributyrate (Sigma-Aldrich, St. Louis MO, USA; protocol can be found here: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/ product/documents/417/921/91015dat.pdf), 5 g peptone, 3 g yeast extract, and 6 drops of filter-sterilized 1:1 Tween 20: deionized water per liter (Sigma-Aldrich). Cellulase activity was tested using carboxymethylcellulose agar (CMC), which is composed of carboxymethylcellulose sodium salt 5 g (Sigma-Aldrich, St. Louis MO, USA), sodium nitrate 1 g, potassium chloride 1g, yeast extract 1 g, dipotassium phosphate 0.5 g, magnesium sulfate heptahydrate 0.5 g, and agar 15 g, pH 7.0., respectively (Batubara et al, 2021). After 72 hours, the cellulase plates were flooded with 0.1% Congo Red (10mL/each plate) and left at room temperature for 20 minutes, followed by washing with 1M NaCl (~30 mL).…”

Moringa oleifera Flour Fermentation as Feed Additive on Egg Quality in Mojosari Ducks with Different Storage Period

“…The high protein content of Moringa leaves contributes to egg weight gain. According to Batubara et al (2021), cellulotic bacteria in the fermentation process can produce cellulase enzymes which function to hydrolyze cellulose, so that it can be easily digested by poultry.…”