Isolation and characterization of cellulolytic bacteria from decomposing rice straw

Sirisena, D.M.; Manamendra, TP

doi:10.4038/jnsfsr.v23i1.5568

Cited by 12 publications

(11 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As detected from congo red method, the isolated strain had endo-β-1, 4-glucanase activity. This was one of the enzymes required for the conversion of cellulose to glucose as reported by Sirisena and Manamendra (1995).…”

Section: Discussionmentioning

confidence: 82%

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Baharuddin

Razak

Hock

et al. 2010

American Journal of Applied Sciences

View full text Add to dashboard Cite

Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% lignin content. The temperature, pH and moisture content of the composting pile at day 7 treatment were 58.3, 8.1 and 65.5°C, respectively. The morphological analysis of the isolated microbes was conducted using Scanning Electron Microscope (SEM) and Gram stain method. The congo red test was conducted in order to detect 1% CMC agar degradation activities. Total genomic DNAs were extracted from approximately 1.0 g of mixed compost and amplified by using PCR primers. The PCR product was sequent to identify the nearest relatives of 16S rRNA genes. The localization of bacteria chromosomes was determined by Fluorescence In Situ Hybridization (FISH) analysis. Results: Single isolated bacteria species was successfully isolated from Empty Fruit Bunch (EFB)-Palm Oil Mill Effluent (POME) compost at thermophilic stage. Restriction fragment length polymorphism profiles of the DNAs coding for the 16S rRNAs with the phylogenetic analysis showed that the isolated bacteria from EFB-POME thermophilic compost gave the highest homology (99%) with similarity to Geobacillus pallidus. The strain was spore forming bacteria and able to grow at 60°C with pH 7. Conclusion: Thermophilic bacteria strain, Geobacillus pallidus was successfully isolated from Empty Fruit Bunch (EFB) and Palm Oil Mil Effluent (POME) compost and characterized.

show abstract

Section: Discussionmentioning

confidence: 82%

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Baharuddin

Razak

Hock

et al. 2010

American Journal of Applied Sciences

View full text Add to dashboard Cite

show abstract

“…Most of the genera examined in prior research have been reported for their cellulose degrading ability. Sirisena and Manamendra (1995) have reported cellulolytic activities of Listeria sp. (along with Enterobacteria and Pseudomonas isolates).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of cellulose degrading efficiency of some fungi and bacteria and their biofilms

Gunathilake¹,

Ratnayake²,

Kulasooriya³

et al. 2013

J. Natn. Sci. Foundation Sri Lanka

View full text Add to dashboard Cite

In industrial applications like cellulosic biofuel production, efficient degradation of cellulose is one of the major concerns. But the efficiencies of cellulose degradation by different types of biofilms are not yet explored. This study was carried out to determine whether the community actions of fungal-bacterial biofilms may increase the rates of cellulose bio-degradation. Cellulolytic fungi and bacteria were isolated from soils, composts and leaf litter and screened for simple sugar production. To identify efficient sugar producing communities, combinations of these isolates were tested in batch cultures of cellulose broth prepared with commercial cellulose. Three different communities; fungal and bacterial monocultures, fungal-bacterial biofilms and fungal mixed cultures were tested. The monoculture of Acremonium sp. was the highest sugar yielder among all tested microorganisms. Among fungal mixed cultures, Acremonium sp. with Fusarium sp. was an effective sugar yielder. Acremonium sp. with a Bacillus sp. was effective among fungal-bacterial biofilms. These three cultures were also inoculated into broth media prepared with Eupatorium odoratum or Panicum maximum or Lantana camara or Mimosa pigra to assess their simple sugar production from plant materials. The highest sugar production was by Acremonium sp. monoculture and the lowest was by the Acremonium sp. with Bacillus sp. biofilm. This was true for all four weeds. Thus, all the fungal-bacterial biofilms tested were comparatively less effective sugar producers.

show abstract

“…Fresh culture (5µL) was inoculated on Petri dishes containing BMS1 supplemented with Carboxymethyl cellulose (1% w/v) or beechwood xylan (1% w/v) and incubated at 50°C during 5 days (Tamariz-Angeles et al 2014) by duplicate. Qualitative cellulolytic and xylanolytic activity was tested using Congo Red methodology (Sirisena & Manamendra 1995). The clear zone around the colony was measured as hydrolysis activities.…”

Section: Selection Of Cellulolytic and Xylanolytic Strains-mentioning

confidence: 99%

Isolation of thermotolerant Bacillus subtilis DCH4 from Chancos hot spring (Carhuaz, Peru) with potential to degrade lignocellulosic agriculture wastes

et al. 2020

View full text Add to dashboard Cite

It was isolated bacteria strains from three different types of samples: fresh water, in situ baits and ex situ enrichment. Serial dilutions were prepared and culture was carried at 50 °C using a Basal-Saline medium. Isolated strains were screened for endoglucanase and xylanase activities with qualitative (Congo Red) and quantitative (DNS) methods. Molecular 16S rDNA sequencing analysis was performed for taxonomic identification. It was isolated 31 strains of which 14 showed hydrolytic activities and belonged to Bacillus subtilis and Bacillus licheniformis species. Moreover, the strain B. subtilis DCH4 showed the highest endoglucanase activity at 45°C and pH 5, and xylanase activity at 55°C and pH 6. Then, DCH4 was cultivated by submerged fermentation with two different media supplemented with sugar cane bagasse, wheat straw, or quinoa stalk to evaluate its saccharification capability. Likewise, it was screening its xylanase and cellulase genes employing specific primers; the amplicons obtained were sequenced, and analyzed. It was found that, enzymatic extracts of DCH4 prepared with cane bagasse or quinoa stalk media achieved the highest endoglucanase and xylanase activities. According to molecular analysis of genes involved in the hydrolytic process, the endoglucanase and xylanase activities exhibited by DCH4 could be attributed to a bifunctional cellulase conformed by endo-beta-1,4-glucanase (GH5) joined to cellulose binding domain 3 (CBM3), and an endo-1,4-beta-xylanase (GH11), respectively. Further transcriptomic experiments would be considered to accomplish optimization strategies for biofuel production from lignocellulosic biomass.

show abstract

Isolation and characterization of cellulolytic bacteria from decomposing rice straw

Cited by 12 publications

References 11 publications

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Evaluation of cellulose degrading efficiency of some fungi and bacteria and their biofilms

Isolation of thermotolerant Bacillus subtilis DCH4 from Chancos hot spring (Carhuaz, Peru) with potential to degrade lignocellulosic agriculture wastes

Contact Info

Product

Resources

About