Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis

Abstract

In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol (cerevisterol) (1), (22E,24S)-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol (2), and indole-3-carboxylic acid (3). The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 1-3 from C. nebularis.

Review: Harvesting therapeutically important bioactive compounds from possible poisonous Clitocybe species

“…Clitocybe nebularis with lectin shows rich, versatile, positive biological activity in human and veterinary medicine. Clitocybe nebularis lectin (CNL), therefore, can be regarded as a promising candidate for different applications in medicine (Yazdani et al, 2020).…”

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

“…Similar results with respect to antimicrobial activity of 60 were obtained by Zhou et al [ 213 ]. On the other hand, no antimicrobial activity for cerevisterol ( 60 ) was reported in works [ 214 , 215 ].…”