Isolation and Characterization of China Isolates of<i>Duddingtonia flagrans</i>, a Candidate of the Nematophagous Fungi for Biocontrol of Animal Parasitic Nematodes

Wang, Bobo; Liu, Wei; Chen, Mingyue; Li, Xuan; Han, Yuan; Xu, Qiang; Sun, Long-Jie; Xie, De-qiong; Cai, Kui-Zheng; Liu, Yi-Zhong; Liu, Junlin; Yi, Lin-xin; Wang, Hui; Zhao, Ming-Wang; Li, Xiaoshan; Wu, Jiayan; Yang, Jing; Wang, Yueying

doi:10.1645/14-715.1

Cited by 27 publications

(19 citation statements)

References 34 publications

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“…Current findings clearly proved nematophagous activity of D. flagrans MUCL 9827 against L3 of sheep GIN. Furthermore, the finding of trophic hyphae and chlamydospores within the body of L3 proved that they were used as a food source by fungal colony since chlamydospores in larval body form after digestion occurred (Wang et al, 2015). The existence of predatory behavior of D. flagrans MUCL 9827 is in accordance with findings of Duddington (1949) who also observed trapping effect during isolation process.…”

Section: Discussionsupporting

confidence: 83%

“…In 1968 CBS deposited the isolate to MUCL in Belgium, where it was designated as MUCL 9827 (http://bccm.belspo.be/catalogues/mucl-strain-details?NUM=9827). The species was named as Duddingtonia flagrans by Cooke (1969) and renamed to Arthrobotrys flagrans by Scholler et al (1999), but similarly to others (Skipp et al, 2002;Wang et al, 2015), we used the name D. flagrans to maintain continuity in publications regarding biological control.…”

Section: Methodsmentioning

confidence: 99%

“…However, in this management system, it is practically impossible to avoid the invasion of different types of parasites that contaminate pastures. Invasion with ubiquitous gastro-intestinal nematodes (GIN) is the most important limiting factor in the intensive sheep breeding around the world, due to economic losses and the specificities of different ways for its control (Vujić et al, 1980;West et al, 2009). Different measures against GIN include controlled grazing, breeding and nutrition strategies, anthelmintic drugs and biological control.…”

Section: Introductionmentioning

confidence: 99%

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Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Simin

Đurić

Kuruca

et al. 2016

Contemporary Agriculture

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Summary Gastro-intestinal nematodes (GIN) of sheep are one of major constraints in grazing production systems worldwide. Control is commonly achieved using anthelmintics, but global occurrence of anthelmintic resistance to different drugs and the emergence of multi-resistant GIN species seriously limit the efficiency of their use. Therefore, integrated parasite management is widely recommended, with nematophagous fungi as one of control tools. Duddingtonia flagrans is one of the most used species, with various effect of different isolates. In previously performed coproculture assay, we showed low efficacy of D. flagrans MUCL 9827 against infective larvae (L3) of sheep GIN. The aim of current experiment was to reevaluate its nematophagous potential, using the medium where direct interaction between the fungus and L3 could be observed. Nematophagous activity was tested on 2% water agar with addition of chloramphenicol on three series of plates seeded with 500 and 1000 chlamidospores and agar blocks with 7 days old mycelium. At Days 0 and 5, 500 L3 of sheep GIN were added to test the trapping activity. The cultures, including control plates with only L3, were incubated at 25°C for 10 days, followed by evaluation of their number and reduction percentage. Nematophagous activity of D. flagrans MUCL 9827 against L3 was clearly demonstrated. However, the overall efficacy was poor since trapping was observed only in one out of nine plates containing fungal material. Potential reasons for such poor performance of the isolate of fungal species, otherwise known as successful in trapping animal parasitic nematodes, are discussed.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Simin

Đurić

Kuruca

et al. 2016

Contemporary Agriculture

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“…As already mentioned, D. flagrans is a widespread biocontrol agent of animal parasites. It can produce large numbers of thick-walled chlamydospores and survive even at high temperatures (50-60 °C) and in hostile environments, such as animal digestive tract (25). It has been verified by the work of Campos et al (26) where different fungal structures of D. flagrans were resistant to the digestive process in goats.…”

Section: Discussionmentioning

confidence: 87%

Acute Oral Toxicity of Vetom 21.77 Based on Duddingtonia Flagrans in Broiler Chickens

Rafikova

2019

Macedonian Veterinary Review

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A 14-d study was undertaken to test the acute toxicity of a new preparation Vetom 21.77 based on the predacious fungus Duddingtonia flagrans. A total of 40 healthy 5-day-old broiler chickens (Hubbard F15, 100 ± 5 g), that had previously gone through a required 5-days adaptation to the environment, were orally dosed with the drug for 5 consecutive days at different doses, after which their health status was assessed daily up to the end of the experiment. According to the results, no substantial changes in the physiological state of the chickens were detected during the experiment. Internal organs weighing revealed no statistically significant differences between the groups, though weight coefficient values of internal organs of treated chickens slightly exceeded those of the control group. Some haematological parameters were significantly higher in the treatment group, without going beyond reference ranges. All chickens used in the experiment survived the study. The preparation has not produced any toxic effect even at a higher dose (4000 µL/kg bw/day). It is concluded that Vetom 21.77 pertains to preparations of IV toxicity class.

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“…The organism also produces chlamydospores. A. superba produces vertical conidiophores, which are 180–600 μm long and are sometimes branched, whereas the apex bears 20–39 conidia, which are elliptical, have one septate, China . In our previous studies (Cai et al unpublished data), A. cookedickinson was characterized and measure 7.5–27.5 (15.8) μm× 5–10.5 (6.6) μm in size.…”

Section: Introductionmentioning

confidence: 98%

In vitro and in vivo studies of the native isolates of nematophagous fungi from China against the larvae of trichostrongylides

Wang

et al. 2016

J Basic Microbiol

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To screen potential nematophagous fungi candidates for the biological control of parasitic nematodes in livestock, in vitro and in vivo studies of the native isolates of nematophagous fungi against the larvae of trichostrongylides were conducted. The in vitro predatory activity of 16 native nematophagous fungal isolates on the larvae of trichostrongylides in sheep feces was assessed. In the ten isolates of Duddingtonia flagrans, the reduction percentage for the infective larvae (L3) of Trichostrongylus colubriformis ranged from 57.21 to 99.83%, and that of Haemonchus contortus ranged from 62.12 to 99.88%. The analysis of the same assay on five isolates of Arthrobotrys superba and one isolate of A. cookedickinson (Monacrosporium cystosporum) showed comparable results with those for D. flagrans. To determine the excretion time of fungal isolates in feces after oral administration, D. flagrans (SDH035) were studied in vivo in sheep and rabbits. Results showed that the tested fungal isolates existed in sheep feces from 12 to 72 h after fungal treatment, and the fungal excretion in rabbit feces occurred at 4 h, reached a peak at 10 h, and declined gradually 18 h after oral administration. All the native fungal isolates were assessed after passing through the gastrointestinal tract of sheep. Treatment with isolates of D. flagrans significantly reduced the number of developing larvae in the feces, and the efficacies ranged from 55.15 to 98.82%. One out of the five isolates of A. superba and A. cookedickinson (BS002) survived after passing through the gastrointestinal tract, and the L3 reduction rates were 83.79 and 81.33%, respectively. Results of the present study provide information about the in vitro predatory activity of nematophagous fungi from China on the L3 of trichostrongylides and their ability to pass through the gastrointestinal tract before administering them for biocontrol.

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Isolation and Characterization of China Isolates ofDuddingtonia flagrans, a Candidate of the Nematophagous Fungi for Biocontrol of Animal Parasitic Nematodes

Cited by 27 publications

References 34 publications

Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Acute Oral Toxicity of Vetom 21.77 Based on Duddingtonia Flagrans in Broiler Chickens

In vitro and in vivo studies of the native isolates of nematophagous fungi from China against the larvae of trichostrongylides

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