Isolation and characterization of Citrobacter spp. from the intestine of grass carp Ctenopharyngodon idellus

Lü, Aijun; Hu, Xiucai; Zheng, Lu; Zhu, Aiguo; Cao, Changqun; Jiang, Jihong

doi:10.1016/j.aquaculture.2011.01.018

Cited by 39 publications

(40 citation statements)

References 13 publications

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“…Under intensive culture conditions, enteritis (or intestinal inflammation) is emerging as a major cause of high mortality in farmed fish, causing huge annual economic losses (Lee et al, 2002;Soffientino et al, 1999). Bacterial enteritis is probably the most common intestinal disease suffered by freshwater fish (Lü et al, 2011;Macpherson et al, 2012;Muroga et al, 1990;Xu et al, 1987Xu et al, , 1988. Among the bacterial pathogens identified thus far, Aeromonas hydrophila is generally considered a major pathogen in almost all animal taxa, and causes intestinal inflammation in fish (Cascón et al, 2000;Xu et al, 1988).…”

Section: Introductionmentioning

confidence: 98%

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

et al. 2014

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Section: Introductionmentioning

confidence: 98%

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

et al. 2014

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“…In fact, inflammation is a normal biological response of the host immune system to noxious stimuli and conditions, such as bacterial infection and tissue injury, which allows for the repair of damaged or infected tissue. In intensive aquaculture, severe and widespread inflammation can develop more readily in the intestines than in other tissues, largely due to a heavier pathogenic bacteria load in the intestinal lumen or a more effective colonization of the intestinal mucosa282930.…”

mentioning

confidence: 99%

A transcriptome analysis focusing on inflammation-related genes of grass carp intestines following infection with Aeromonas hydrophila

Song

Sun

et al. 2017

Sci Rep

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Inflammation is a protective response that is implicated in bacterial enteritis and other fish diseases.The inflammatory mechanisms behind Aeromonas hydrophila infections in fish remain poorly understood. In this study, we performed a de novo grass carp transcriptome assembly using Illumina's Solexa sequencing technique. On this basis we carried out a comparative analysis of intestinal transcriptomes from A. hydrophila-challenged and physiological saline solution (PSS/mock) -challenged fish, and 315 genes were up-regulated and 234 were down-regulated in the intestines infected with A. hydrophila. The GO enrichment analysis indicated that the differentially expressed genes were enriched to 12, 4, and 8 GO terms in biological process, molecular function, and cellular component, respectively. A KEGG analysis showed that 549 DEGs were involved in 165 pathways. Moreover, 15 DEGs were selected for quantitative real-time PCR analysis to validate the RNA-seq data. The results confirmed the consistency of the expression levels between RNA-seq and qPCR data. In addition, a time-course analysis of the mRNA expression of 12 inflammatory genes further demonstrated that the intestinal inflammatory responses to A. hydrophila infection simultaneously modulated gene expression variations. The present study provides intestine-specific transcriptome data, allowing us to unravel the mechanisms of intestinal inflammation triggered by bacterial pathogens.The grass carp (Ctenopharyngodon idella) is an intensively cultured and economically important herbivorous fish species in China. In grass carp aquaculture, the fish are often infected by bacterial, viral and parasitic pathogens. Aeromonas hydrophila, a Gram-negative aquatic bacterium, is regarded as the major bacterial pathogen causing intestinal inflammation in animal taxa, including farmed fish 1-4 . A. hydrophila-induced enteritis is probably the most widespread disease in grass carp, especially under intensive rearing conditions, which results in huge economic losses annually due to reduced growth and high mortality rates [5][6][7] . The molecular mechanisms and pathways that regulate bacterial-induced inflammatory processes in humans and other mammalian species are relatively well understood [8][9][10] . In fish, several inflammation-related genes, including chemokine (C-X-C motif) ligand 2 (CXCL2) 11 , CXCL10 12 , cyclooxygenase-2 (COX-2) 13 , interleukin-1β (IL-1β ) 4,13,14 , IL-8 15,16 , IL-17A/F2 17 , and tumor necrosis factor (TNF)-α 13,18 , have already been identified and characterized with regard to their involvement in inflammatory responses to bacterial infections. However, the definitive mechanisms responsible for the bacterial-induced intestinal inflammation in fish are still unclear. Therefore, further studies are required to identify more molecular components that mediate inflammatory reactions in fish.In the past few years, transcriptome sequencing technology has found broad applications in gene discovery and gene expression profiling. Recently, a number of studi...

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“…Although the skin is believed to play a pivotal role in fish immune responses, a few studies have paid attention to it [8e13]. We report here that adult zebrafish were infected by immersion exposure with bacterium C. freundii as previously described [18]. More important, the transcription profile of the skin of infected zebrafish was successfully detected by microarray analysis, which shows the evidence that the use of zebrafish as an immunological model is a valuable platform to explore the interactions between the skin and bacteria, and will also be used to further study the prevention and cure of C. freundii infection.…”

Section: Discussionmentioning

confidence: 85%

“…Adult zebrafish were infected by immersion with C. freundii as previously described [18]. Zebrafish were acclimatized in aerated fresh water tanks for 15 d prior to the experiment at a water temperature of 25 C, and were fed with commercial dry feed distributed manually twice a day.…”

Section: Freundii Infection In Zebrafishmentioning

confidence: 99%

“…Recently, infection of zebrafish has been successfully demonstrated with some bacterial pathogens [15e17]. Very recently, zebrafish as a model for the evaluation of pathogenicity of Citrobacter freundii has been performed by our group, which exhibited features with the developed diffuse bleeding on the skin [18]. In this study, microarray analysis was used to identify transcription profiles of the skin of C. freundiiinfected zebrafish, with the aim of clarifying the mechanism of skin immune responses against bacterial pathogens in fish.…”

Section: Introductionmentioning

confidence: 99%

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Gene expression profiling in the skin of zebrafish infected with Citrobacter freundii

Lü

Xue

et al. 2012

Fish & Shellfish Immunology

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Isolation and characterization of Citrobacter spp. from the intestine of grass carp Ctenopharyngodon idellus

Cited by 39 publications

References 13 publications

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella): An experimental model

A transcriptome analysis focusing on inflammation-related genes of grass carp intestines following infection with Aeromonas hydrophila

Gene expression profiling in the skin of zebrafish infected with Citrobacter freundii

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