Isolation and Characterization of Crude Oil Degrading Bacteria in Association with Microalgae in Saver Pit from Egbaoma Flow Station, Niger Delta, Nigeria

Abstract

<jats:p>The capability of indigenous bacteria and microalgae in crude oil effluents to grow in and utilize crude oil as their sole source of carbon and energy provides an environmentally friendly and economical process for dealing with crude oil pollution and its inherent hazards. In view of the toxicity of crude oil spillages to indwellers of the affected ecosystems and the entire affected environment, the isolation of pure bacterial and microalgae cultures from crude effluents is a step in the right direction, particularly for bio-augmentation or bioremediation purposes. The total heterotrophic bacteria count and hydrocarbon utilizing bacteria count, as well as the microalgae count, were determined with the pour plate technique. The physicochemical properties of the effluent samples were also analyzed. Identification of the hydrocarbon utilizing bacteria was performed with phenotypic techniques. The result shows a mean total heterotrophic bacterium count of 5.91 log CFU/ml and a mean microalga count of 4.77 log cells/ml. When crude oil and polycyclic aromatic hydrocarbon (PAH) were used as sole carbon sources, total hydrocarbon utilizing bacteria counts were respectively estimated at 3.89 and 2.89 log CFU/ml. Phenotypic identification of hydrocarbon utilizing bacteria in the crude oil effluents revealed the presence of two main bacterial genera: Streptococcus and Pseudomonas. Data obtained from this study confirmed the biodegradative abilities of indigenous bacterial species, thus, ultimately resulting in the amelioration of the toxicity associated with the crude oil effluents.</jats:p>

Biokinetic Study of Microbial Decontamination of Oilfield Produced Water

“…The biochemical test carried out to identify the five strains showed that they belonging to the Bacillus species, Pseudomonas species and Chryseobacterium Species. The result agrees with other studies that reported the ability of these species in degrading petroleum hydrocarbons [12,16,17].…”

Characterization of Autochthonous Bacterial Isolates with The Potentiality to Degrade Spent Engine Oil from Contaminated Soil Area Enriched with Glycine max

“…Among the endowed metabolic properties enabling them to degrade these compounds include; tolerance to high concentrations of solvents, embodiment of complimentary metabolic capacity through rapid alteration and the exchange of genetic material, possession of specific enzyme system for oxidation of organic compounds by which molecular oxygen is incorporated by dioxygenases to form cisdihydrodiols. The intermediates of dihydrodiol dehydrogenation are metabolized into carbon dioxide and water through the catechols by the action of catechol dioxydrogenases and other enzymes (Chikere et al, 2011;Akpoka et al, 2020). Consequently, from the pool of the total heterotrophic bacteria count, the HBC mean ranged from 2.86 + 0.08 log10 CFU/g to 5.76+ 0.26 log10 CFU/g with the highest HBC count (5.76+ 0.26 log10 CFU/g) recorded from contaminated soil enriched with Glycine max and lowest count was obtained from the uncontaminated soil (Table 2).…”