1981
DOI: 10.1128/mcb.1.2.83
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Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.

Abstract: Seven dominant mutations showing greatly enhanced resistance to the glucose repression of galactokinase synthesis have been isolated from GAL81 mutants, which have the constitutive phenotype but are still strongly repressible by glucose for the synthesis of the Leloir enzymes. These glucose-resistant mutants were due to semidominant mutations at either of two loci, GAL82 and GAL83. Both loci are unlinked to the GAL81-gal4, gal80, or gal7-gallO.-gall locus or to each other. The GAL83 locus was mapped on chromos… Show more

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Cited by 95 publications
(121 citation statements)
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“…However several lines of evidence indicate that, in yeast, catabolite repression is not associated with a low level of CAMP. In a mutant permeable to CAMP, the addition of exogenous cAMP did not prevent glucose repression of galactokinase synthesis (Matsumoto et al, 1982) and in different yeasts intracellular concentrations of cAMP are higher in the presence of glucose and other sugars than in derepressed conditions (Eraso and Gancedo, 1984). Results from strains lacking adenylate cyclase suggest that, in S. certvisiae, cAMP itself does not play a critical role in catabolite repression (Matsumoto et al, 1983a) although cAMP may be necessary for the transcription of certain genes like SUC2 (Matsumoto et al, 1984).…”
Section: The Signalmentioning
confidence: 86%
“…However several lines of evidence indicate that, in yeast, catabolite repression is not associated with a low level of CAMP. In a mutant permeable to CAMP, the addition of exogenous cAMP did not prevent glucose repression of galactokinase synthesis (Matsumoto et al, 1982) and in different yeasts intracellular concentrations of cAMP are higher in the presence of glucose and other sugars than in derepressed conditions (Eraso and Gancedo, 1984). Results from strains lacking adenylate cyclase suggest that, in S. certvisiae, cAMP itself does not play a critical role in catabolite repression (Matsumoto et al, 1983a) although cAMP may be necessary for the transcription of certain genes like SUC2 (Matsumoto et al, 1984).…”
Section: The Signalmentioning
confidence: 86%
“…Acid phosphatase was unaffected by disruption of snf1, and loss of SNF1 also had little effect on the constitutive expression of acid phosphatase in a pho85 strain. Mutation of REG1, a gene that is necessary for glucose repression (11,12,42,43,48,79), caused some increase in acid phosphatase expression, though to levels well short of those associated with a pho85-null mutation (Table 2). We conclude that PHO85 and SNF1 act for the most part independently to control phosphate utilization and glucose repression but interact strongly in regulating glycogen deposition.…”
Section: Resultsmentioning
confidence: 99%
“…The Reg1-Glc7 complex acts in opposition to the Snf1 signaling pathway by promoting the dephosphorylation of Snf1 threonine 210 (21). On glucose medium, reg1⌬ mutant strains exhibit derepressed levels of invertase and galactokinase (20,23) To test this prediction, we crossed pak1⌬ and reg1⌬ mutant strains and characterized the phenotypes of the pak1⌬ reg1⌬ double mutant. We found that deletion of PAK1 effectively suppressed a number of the phenotypes of reg1⌬.…”
Section: Resultsmentioning
confidence: 99%