Isolation and characterization of eight polymorphic microsatellite loci for Natterer’s bat, Myotis nattereri (Vespertilionidae, Chiroptera)

Scott, David D.; Fitzpatrick, Seán; Bailie, Deborah A.; Boston, Emma S. M.; Lundy, Mathieu G.; Buckley, Daniel J.; Teeling, Emma C.; Montgomery, W. Ian; Prodöhl, Paulo A.

doi:10.1007/s12686-013-9871-2

Cited by 5 publications

(3 citation statements)

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“…Individuals were genotyped using 19 nuclear microsatellite markers and one mitochondrial microsatellite marker. The following nuclear markers were used: EF15 [32]; b22 [33]; A2-Mluc, A13-Mluc, E5-Mluc, G6-Mluc, G30-Mluc, G31-Mluc [34], D15, H19, H23, H29 [35]; Mnatt-1, Mnatt-2 [36]; Mschreib3 [37]; Mnatt-8, Mnatt-11 [38]; FV5AP [39] and GZBYR (5′-TCCTTGTCACTATAAGCTCAGTGG-3′ (forward); 5′-CCAGGCAATAGTCTCCTAGCAC-3′ (reverse)). The 5′ end of the reverse primers FV5AP and G30-Mluc were PIG-tailed [40] with the sequence GTTT and the 5′ end of the reverse primer G31-Mluc with the sequence GTTTT to facilitate adenylation.…”

Section: Methodsmentioning

confidence: 99%

Evidence for genetic variation in Natterer’s bats (Myotis nattereri) across three regions in Germany but no evidence for co-variation with their associated astroviruses

et al. 2017

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BackgroundAs bats have recently been described to harbor many different viruses, several studies have investigated the genetic co-variation between viruses and different bat species. However, little is known about the genetic co-variation of viruses and different populations of the same bat species, although such information is needed for an understanding of virus transmission dynamics within a given host species. We hypothesized that if virus transmission between host populations depends on events linked to gene flow in the bats, genetic co-variation should exist between host populations and astroviruses.ResultsWe used 19 nuclear and one mitochondrial microsatellite loci to analyze the genetic population structure of the Natterer’s bat (Myotis nattereri) within and among populations at different geographical scales in Germany. Further, we correlated the observed bat population structure to that of partial astrovirus sequences (323–394 nt fragments of the RNA-dependent RNA polymerase gene) obtained from the same bat populations. Our analyses revealed that the studied bat colonies can be grouped into three distinct genetic clusters, corresponding to the three geographic regions sampled. Furthermore, we observed an overall isolation-by-distance pattern, while no significant pattern was observed within a geographic region. Moreover, we found no correlation between the genetic distances among the bat populations and the astrovirus sequences they harbored. Even though high genetic similarity of some of the astrovirus haplotypes found in several different regions was detected, identical astrovirus haplotypes were not shared between different sampled regions.ConclusionsThe genetic population structure of the bat host suggests that mating sites where several local breeding colonies meet act as stepping-stones for gene flow. Identical astrovirus haplotypes were not shared between different sampled regions suggesting that astroviruses are mostly transmitted among host colonies at the local scale. Nevertheless, high genetic similarity of some of the astrovirus haplotypes found in several different regions implies that occasional transmission across regions with subsequent mutations of the virus haplotypes does occur.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0856-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Evidence for genetic variation in Natterer’s bats (Myotis nattereri) across three regions in Germany but no evidence for co-variation with their associated astroviruses

et al. 2017

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“…The aim of our study was to explore the relatedness of individuals within and across each of these scales. We test the hypotheses using a suite of microsatellite markers developed for Natterer's bat (Puechmaille et al 2012;Rivers et al 2005;Scott et al 2013) to characterise the genetic diversity and structure present in colonies of Natterer's bat in northern England. We assessed gene flow to consider the scale at which conservation management needs to be considered.…”

Section: Supplementary Informationmentioning

confidence: 99%

“…This may be caused by the presence of null alleles. However, Mnatt-10 was retained in the analysis as the locus has been found to have no null alleles in other studies involving Natterer's bats (Halczok et al 2017;Scott et al 2013;Stumpf et al 2017). In addition, analyses with and without this locus lead to the same conclusion.…”

Section: Microsatellite Analysismentioning

confidence: 99%

Population structure, gene flow and relatedness of Natterer’s bats in Northern England

Mordue

Aegerter²,

Mill

et al. 2021

Mamm Biol

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There have been significant declines in population numbers of many bat species in the United Kingdom, including Natterer’s bats Myotis nattereri, over the last century, largely due to anthropogenic changes. The philopatry, which temperate-zone bats often exhibit to their natal landscapes, in combination with anthropogenic threats, can lead to fragmentation, isolation and sub-division of populations. This may result in bottlenecks and declines in genetic diversity. Multi-scaled research is required to disentangle how the variation in the physical traits of bat species (e.g. affecting flight), as well as their social and behavioural traits (e.g. community size, migration, breeding systems), may affect the genetic health of populations and provide a potential buffer against fragmentation. We used microsatellite markers to characterise the genetic diversity and population structure present in Natterer’s bat colonies to determine whether summer roosting bat colonies were spatially differentiated or part of a meta-population. Analyses of population structure and measures of genetic relatedness suggest spatially differentiated populations of bats exhibit long term site fidelity to summer roosting sites, whilst high genetic diversity at sites indicates gene exchange occurs via swarming sites. Natterer’s bats in northern England may travel greater distances to swarming sites than has been previously documented.

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Relatedness, parentage, and philopatry within a Natterer's bat (Myotis nattereri) maternity colony

et al. 2018

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Given their cryptic behaviour, it is often difficult to establish kinship within microchiropteran maternity colonies. This limits understanding of group formation within this highly social group. Following a concerted effort to comprehensively sample a Natterer's bat (Myotis nattereri) maternity colony over two consecutive summers, we employed microsatellite DNA profiling to examine genetic relatedness among individuals. Resulting data were used to ascertain female kinship, parentage, mating strategies, and philopatry. Overall, despite evidence of female philopatry, relatedness was low both for adult females and juveniles of both sexes. The majority of individuals within the colony were found to be unrelated or distantly related. However, parentage analysis indicates the existence of a number of maternal lineages (e.g., grandmother, mother, or daughter). There was no evidence suggesting that males born within the colony are mating with females of the same colony. Thus, in this species, males appear to be the dispersive sex. In the Natterer's bat, colony formation is likely to be based on the benefits of group living, rather than kin selection.

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Isolation and characterization of eight polymorphic microsatellite loci for Natterer’s bat, Myotis nattereri (Vespertilionidae, Chiroptera)

Cited by 5 publications

References 8 publications

Evidence for genetic variation in Natterer’s bats (Myotis nattereri) across three regions in Germany but no evidence for co-variation with their associated astroviruses

Evidence for genetic variation in Natterer’s bats (Myotis nattereri) across three regions in Germany but no evidence for co-variation with their associated astroviruses

Population structure, gene flow and relatedness of Natterer’s bats in Northern England

Relatedness, parentage, and philopatry within a Natterer's bat (Myotis nattereri) maternity colony

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