Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions

Abstract

Endophytes are regarded with immense potentials in terms of plant growth promoting (PGP) elicitors and mimicking secondary metabolites of medicinal importance. Here in the present study, we explored Bacopa monnieri plants to isolate, identify fungal endophytes with PGP elicitation potentials, and investigate secretion of secondary metabolites such as bacoside and withanolide content under in vitro conditions. Three fungal endophytes isolated (out of 40 saponin producing isolates) from leaves of B. monnieri were examined for in vitro biosynthesis of bacosides. On morphological, biochemical, and molecular identification (ITS gene sequencing), the isolated strains SUBL33, SUBL51, and SUBL206 were identified as Nigrospora oryzae (MH071153), Alternaria alternata (MH071155), and Aspergillus terreus (MH071154) respectively. Among these strains, SUBL33 produced highest quantity of Bacoside A 3 (4093 μg mL −1 ), Jujubogenin isomer of Bacopasaponin C (65,339 μg mL −1 ), and Bacopasaponin C (1325 μg mL −1 ) while Bacopaside II (13,030 μg mL −1 ) was produced by SUBL51 maximally. Moreover, these aforementioned strains also produced detectable concentration of withanolides—Withaferrin A, Withanolide A (480 μg mL −1 ), and Withanolide B (1024 μg mL −1 ) respectively. However, Withanolide A was not detected in the secondary metabolites of strain SUBL51. To best of our knowledge, the present study is first reports of Nigrospora oryzae as an endophyte in B. monnieri with potentials of biosynthesis of economically important phytomolecules under in vitro conditions. Supplementary Information The online version contains supplementary material available at 10.1007/s42770-021-00586-0.

Global regulatory factor FsveA up-regulate the production of antitumor substance in endophytic Fusarium solani

“…Sequencing was done on the PCR products. After performing the nucleotide BLAST of sequencing product, the fasta sequence of similar organisms along with nearest neighbor sequences from the NCBI database were download (Table S1) and comparison by online server MAFFT v.7.110 (Katoh and Standley, 2013;Soni et al 2021;Ameen et al 2021). The spliced dataset (Vaidya et al 2011) was analyzed phylogenetically using the online software CIPRES Science Gateway v.3.3 (Assunção et al 2020).…”

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

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“…Endophytes are microorganisms that live in the plant's endosphere but do not cause disease symptoms (Mishra and Priyanka, 2022). They colonize the plant tissue, either inter or intracellularly, and maintain a harmonic symbiotic relationship in all of the plants studied (Soni et al, 2021).…”

Management of chromium(VI)-contaminated soils through synergistic application of vermicompost, chromate reducing rhizobacteria and Arbuscular mycorrhizal fungi (AMF) reduced plant toxicity and improved yield attributes in Ocimum basilicum L.

“…Globally a large population (80%) still relay on herbal products, supplements for their primary healthcare and immune boosting (Ekor 2014 ; Soni et al 2022 ). In the time of COVID-19 pandemic, demands and usage of herbal supplements and drugs are ever-increasing (Soni et al 2021 , 2022 ). Particularly in Indian subcontinent, masses still relay on traditional system of medicine obtained directly from medicinal plants for maintenance of their day-to-day life and immune boosting (Soni et al 2021 ).…”

“…In the time of COVID-19 pandemic, demands and usage of herbal supplements and drugs are ever-increasing (Soni et al 2021 , 2022 ). Particularly in Indian subcontinent, masses still relay on traditional system of medicine obtained directly from medicinal plants for maintenance of their day-to-day life and immune boosting (Soni et al 2021 ). The medicinal plants contain high economical value secondary metabolites which either directly or indirectly used as raw materials for pharmaceutical, cosmetic and perfumery industries (Tiwari et al 2017 , 2021 ).…”