Isolation and characterization of Endophytic-Rhizospheric phosphate solubilizing bacteria of cumin and their evaluation in vitro

Abstract

The present investigation was carried out during the year 2017-18 and 2018-19 in Laboratory of Microbiology, Devision of crop production, ICAR-National Research Centre for Seed spices Ajmer, Rajasthan (India). The maximum P solubilization zone was 19 mm followed by 17 mm recorded in isolate DCU-251 which was characterized as Pseudomonas aeruginosa (Accession no. MN192165) and DCU-262 (Kosakonia oryzendophytica strain NRCSSDCU262 Accession no. MN192166), respectively. The least solubilization zone was 11 mm due to isolate DCU-551. Phosphate solubilization index ranged 1.6-3.1. Highest SI was 3.1 followed by 2.9 and 2.8 associated with isolate DCU-251(Pseudomonas aeruginosa strain NRCSSDCU251 Accession no. MN192165), DCU-262 (Kosakonia oryzendophytica strain NRCSSDCU262 Accession no. MN192166) and DCU-22 (Bacillus paramycoides strain NRCSSDCU22 Accession no. MN192162), respectively. The Lowest value of SI was 1.5 due to isolate DCU-551. The highest soluble P (326 g ml −1 ) in pikovskaya's broth was recorded in bacterial isolate DCU-251(Pseudomonas aeruginosa strain NRCSSDCU251 Accession no. MN192165) and least soluble P was 152 g ml −1 recorded in isolate DCU-553.

Isolation, Characterization, and Evaluation of Native Rhizobacterial Consortia Developed From the Rhizosphere of Rice Grown in Organic State Sikkim, India, and Their Effect on Plant Growth

“…polymyxa COW3. Our results are in agreement with studies by Di Benedetto et al (2019) and Devi et al (2020) , who had showed the PGP properties of Bacillus spp., Pseudomonas spp., and Kosakonia spp. Panneerselvam et al (2019) showed that B. subtilis strain BioCWB (570 μg ml –1 ) and B. luciferensis strain K2 (417.3 μg ml –1 ) produced higher amounts of phosphates.…”

“…The collective PGP traits and nutrient solubilization properties observed in Consortia-3 were due to the bacterial mixture of K.oryzendophytica YMA7, P. kribbensis KSB, and P. polymyxa COW3. Our results are in agreement with studies by Di Benedetto et al (2019) and Devi et al (2020), who had showed the PGP properties of Bacillus spp., Pseudomonas spp., and Kosakonia spp. Panneerselvam et al (2019) showed that B. subtilis strain BioCWB (570 µg ml −1 ) and B. luciferensis strain K2 (417.3 µg ml −1 ) produced higher amounts of phosphates.…”

“…It also produced siderophores. It also produced a high amount of phosphate (517 μg ml –1 ) as compared with K. oryzendophytica strain NRCSSDCU262 (207 μg ml –1 ), which is an endophytic-rhizospheric phosphate-solubilizing bacteria (PSB) isolated from cumin grown in agricultural fields of Rajasthan, India ( Devi et al, 2020 ).…”

“…( P. kribbensis , P. rhodesiae ), Burkholderia spp., and Kosakonia spp. possessed PGP attributes, enhanced plant growth, and increased yield in several agricultural and horticultural crops ( Park et al, 2017 ; Devi et al, 2020 ; Castanheira et al, 2015 ).…”