Isolation and characterization of ethanol tolerant yeast strains

Tikka, Chiranjeevi; Osuru, Hari Prasad; Atluri, Navya; Raghavulu, Praveen; Yellapu, Nanda Kumar; Mannur, Ismail Shaik; Prasad, Uppu Venkateswara; Aluru, Sudheer; K, Narasimha Varma; Bhaskar, Matcha

doi:10.6026/97320630009421

Cited by 67 publications

(49 citation statements)

References 7 publications

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“…To test for ethanol tolerance, the selected thermotolerant yeast isolates were inoculated into YM medium containing 7, 10 and 13% (v/v) ethanol 39 . The majority of the isolates tolerated ethanol concentrations up to 10% (v/v) (Fig.…”

Section: Resultsmentioning

confidence: 99%

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Techaparin

Thanonkeo

Klanrit

2017

Brazilian Journal of Microbiology

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The application of high-potential thermotolerant yeasts is a key factor for successful ethanol production at high temperatures. Two hundred and thirty-four yeast isolates from Greater Mekong Subregion (GMS) countries, i.e., Thailand, The Lao People's Democratic Republic (Lao PDR) and Vietnam were obtained. Five thermotolerant yeasts, designated Saccharomyces cerevisiae KKU-VN8, KKU-VN20, and KKU-VN27, Pichia kudriavzevii KKU-TH33 and P. kudriavzevii KKU-TH43, demonstrated high temperature and ethanol tolerance levels up to 45 °C and 13% (v/v), respectively. All five strains produced higher ethanol concentrations and exhibited greater productivities and yields than the industrial strain S. cerevisiae TISTR5606 during high-temperature fermentation at 40 °C and 43 °C. S. cerevisiae KKU-VN8 demonstrated the best performance for ethanol production from glucose at 37 °C with an ethanol concentration of 72.69 g/L, a productivity of 1.59 g/L/h and a theoretical ethanol yield of 86.27%. The optimal conditions for ethanol production of S. cerevisiae KKU-VN8 from sweet sorghum juice (SSJ) at 40 °C were achieved using the Box–Behnken experimental design (BBD). The maximal ethanol concentration obtained during fermentation was 89.32 g/L, with a productivity of 2.48 g/L/h and a theoretical ethanol yield of 96.32%. Thus, the newly isolated thermotolerant S. cerevisiae KKU-VN8 exhibits a great potential for commercial-scale ethanol production in the future.

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Section: Resultsmentioning

confidence: 99%

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Techaparin

Thanonkeo

Klanrit

2017

Brazilian Journal of Microbiology

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“…Isolation of yeasts was carried out by using standard methodology described by Tikka et al (2013) and Zaky et al (2014). After incubation, colonies were selected based on morphology and sub-cultured on yeast extract peptone dextrose (YPD) agar slants for further observations.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

et al. 2017

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The purpose of this study is to evaluate the probiotic characteristics of 15 yeast strains isolated from nectar of toddy palm. Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized. Commercial probiotic yeast, Saccharomyces boulardii served as the control in these experiments. Of the 15 yeast strains, the isolates that were resistant to antibiotics and worked synergistically with other cultures were considered for further evaluation. Selected isolates were evaluated in vitro for tolerance to simulated gastrointestinal conditions such as temperature, pH, bile and gastric juice. Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells. The 2 yeast isolates with efficient probiotic properties were finally characterized by sequencing their 5.8 S rRNA and partial sequences of internal transcribed spacer 1 and 2. The sequences were BLAST searched in the National Center for Biotechnology Information, nucleic acid database for sequence similarity of organisms and phylogenetic evolutionary analysis was carried out. Based on maximum similarity of basic local alignment search tool results, organisms were characterized as Pichia kudriavzevii OBS1 (100%) and Saccharomyces cerevisiae OBS2 (96%) and sequences were finally deposited in the GenBank data library. Among these two isolates, S. cerevisiae OBS2 displayed slight/moderate antioxidant and anticancer property. Hence, strain OBS2 can be utilized and explored as a potential probiotic for therapeutic applications.

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“…Bioethanol production by fermentation has received widespread interest as a source of renewable energy. Ethanol appeared as an environmentally friendly alternative reducing the adverse effects and increasing costs of lead gasoline on human health (Sumari et al, 2010;Ghassem et al, 2012;Tikka et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Screening of yeast isolates from flowers for effective ethanol production

Chaudhary

Karita

2017

Turk J Biol

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The use of alternative substrates to produce biofuel is a striking option nowadays. This study aimed to screen bioethanolproducing yeast strains. From different flowers, 65 yeasts were isolated. Twelve isolates assimilated glucose by liberation of CO 2 anaerobically. Out of these, only 5 yeast isolates fermented glucose in medium consisting of 0.8 g/L Mg 2+ ions to produce 2.05 ± 0.03% ethanol. The selected five strains were identified as members of the genera Metschnikowia or Meyerozyma based on molecular characterization. Selected yeast strains were used for conversion of rice into bioethanol following dilute acid hydrolysis and fermentation. Consistent ethanol production was 1.80 ± 0.05% at days 2-4 by Metschnikowia cibodasensis Y34 and 2.20 ± 0.21% by Meyerozyma caribica Y42 at days 4-6 with a gradual decrease at the time of experiment termination (day 10). Metschnikowia cibodasensis Y34 and Meyerozyma caribica Y42 produced the highest ethanol at pH 3, i.e. 1.75 ± 0.14% at days 3 and 5 and 2.05 ± 0.14% at days 1 and 3, respectively, upon incubation with different pH levels and 1% NaCl. Growth and ethanol production at pH 4 and 5 was close to that at pH 3, with a slight increase in production by Metschnikowia cibodasensis Y34 at pH 4 up to day 3.

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Isolation and characterization of ethanol tolerant yeast strains

Cited by 67 publications

References 7 publications

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

Screening of yeast isolates from flowers for effective ethanol production

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