Isolation and Characterization of Factors in Sweet Potato Root Which Agglutinate Germinated Spores of <i>Ceratocystis fimbriata</i>, Black Rot Fungus

Kojima, Mineo; Uritani, Ikuzô

doi:10.1104/pp.62.5.751

Cited by 14 publications

(2 citation statements)

References 23 publications

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“…Ion-exchange treatment of the crude root components results in a lower-molecular-weight fraction which agglutinates almost all pseudomonads examined, with only slight variations in efficacy. A similar phenomenon was observed for strains of the fungus Ceratocystis fimbriata in their agglutination reactions with crude and purified extracts of sweet potato roots (17). The authors demonstrated the presence of a factor(s) in the crude root extract which was able to suppress agglutination reactions of the spores differentially and which probably was lost during the purification procedure.…”

Section: Discussionsupporting

confidence: 72%

Agglutination, Adherence, and Root Colonization by Fluorescent Pseudomonads

Glandorf

Sluis

Anderson

et al. 1994

Appl Environ Microbiol

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Two fractions of agglutination activity towards fluorescent pseudomonads were detected in root washes of potato, tomato, wheat, and bean. High-molecular-mass (>106 Da) components in crude root washes agglutinated only particular saprophytic, fluorescent Pseudomonas isolates. Ion-exchange treatment of the crude root washes resulted in preparations of lower-molecular-mass (105 to 106 Da) fractions which agglutinated almost all Pseudomonas isolates examined. Also, components able to suppress agglutination reactions of pseudomonads with the lower-molecular-mass root components were detected in crude root washes of all crops studied. Pseudomonas isolates were differentially agglutinated by both types of root components. The involvement of these two types of root components in short-term adherence and in colonization was studied in potato, tomato, and grass, using Pseudomonas isolates from these crops. Short-term adherence of isolates to roots was independent of their agglutination with either type of root components. With agglutination-negative mutants, the high-molecular-mass components seemed to be involved in adherence of Pseudomonas putida Corvallis to roots of all crops studied. Short-term adherence to roots of four Pseudomonas isolates could be influenced by addition of both crude and ion-exchange-treated root washes, depending on their agglutination phenotype with these root wash preparations. Potato root colonization by 10 different isolates from this crop, over a period of 7 days, was not correlated with their agglutination phenotype. Aggmutants of P. putida Corvallis were not impaired in root colonization. It is concluded that the root agglutinins studied can be involved in short-term adherence of pseudomonads to roots but do not play a decisive role in their root colonization.

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Section: Discussionsupporting

confidence: 72%

Agglutination, Adherence, and Root Colonization by Fluorescent Pseudomonads

Glandorf

Sluis

Anderson

et al. 1994

Appl Environ Microbiol

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“…The factor was not inactivated by pronase-treatment in contrast to the slight inactivation reported in preceding papers. 2 , 3) This discrepancy in the effect of pronasetreatment might be ascribed to the presence of contaminating protein in the specimen in the preceding work, of which the degradative products interfered with agglutination. Moreover, the factor treated with pronase showed the same behavior as that of the non-treated one on DEAE-Sephacel column chromatography.…”

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confidence: 98%