Isolation and characterization of fifteen polymorphic microsatellite loci for the citrus mealybug, Planococcus citri (Hemiptera: Pseudococcidae), and cross-amplification in two other mealybug species

Martins, Raquel A.; Zina, Vera; Silva, Elsa Borges da; Rebelo, María Teresa; Figueiredo, Elisabete; Mendel, Zvi; Paulo, Octávio S.; Franco, José Carlos; Seabra, Sofia G.

doi:10.1007/s12041-012-0162-6

Cited by 10 publications

(22 citation statements)

References 15 publications

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“…Thus, the reasons for deviating from the HWE by A. spiniferus may be due to other factors such as mutation, natural selection, small population size (genetic drift and population bottle necks) or presence of population substructures including Wahlund's effect (http:// www.dorak.info/genetics/popgen.html). Specifically, Martins et al (2012) reported that 11 of the 15 microsatellite loci in Planococcus citri (Hemiptera: Pseudococcidae) significantly deviated from the HWE, probably due to data structuring, since individuals from 2 significantly distant geographic areas were used. Probably this is a potential factor affecting the HWE for A. spiniferus, since we used 48 females from Jiangsu (N 32.41° E 119.42°) and Sichuan (N 30.57° E 104.07°) provinces for testing.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite Markers forAleurocanthus spiniferus(Hemiptera: Aleyrodidae) and Their Potential use in Whiteflies

Tang

Tao

Wang

et al. 2014

Florida Entomologist

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Section: Discussionmentioning

confidence: 99%

Microsatellite Markers forAleurocanthus spiniferus(Hemiptera: Aleyrodidae) and Their Potential use in Whiteflies

Tang

Tao

Wang

et al. 2014

Florida Entomologist

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“…Mitochondrial molecular data have helped immensely in understanding the genealogies or phylogenies and also geographical distribution to some extent (Sunnucks, 2000;Coeur d'Acier et al, 2007). In silico mining of microsatellites using EST sequences is considered more economical compared with the higher cost involved in development of microsatellites through next-generation sequencing (Martins et al, 2012). As expressed sequence tag (EST) microsatellites are developed from expressed regions, these regions are expected to be conserved across species compared to neutral microsatellites resulting in better transferability of the EST-based microsatellites.…”

Section: Introductionmentioning

confidence: 99%

In silico mining of microsatellites and analysis of genetic diversity among inter‐ and intra‐generic aphids of the subfamily Aphidinae

Sridhar

Venkatesan

Joshi

et al. 2016

Entomologia Exp Applicata

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Nearly 5 000 aphid species damage crops, either by sucking plant sap or as disease-transmitting vectors. Microsatellites are used for understanding molecular diversity and eco-geographical relationships among aphid species. Expressed sequence tag (EST)-microsatellite motifs were identified through an in silico approach using inbuilt simple sequence repeat mining tools in aphid EST dataset. Microsatellite mining revealed one in every five aphid genes as containing a repeat motif, and out of 9 290 EST microsatellites mined from Aphis gossypii Glover and Acyrthosiphon pisum (Harris) (both Hemiptera: Aphididae), 80% were of A and/or T (AT, ATA, AAT, AATA, and ATTT) motifs, and the rest contained G and/or C motifs. All microsatellite sequences were annotated using BLAST. Primers for EST microsatellites were designed using the Primer 3.0 tool. 106 primer pairs of both dinucleotide repeats (DNRs) and trinucleotide repeats (TNRs), representing open reading frames (ORFs) and untranslated regions (UTRs), were synthesized to amplify 15 aphid species belonging to the subfamily Aphidinae, collected from diverse hosts. Four hundred forty-five polymorphic alleles were amplified. Fifty TNR and 23 DNR microsatellites amplified across the species studied. Polymorphism information content values of microsatellites ranged from 0.23 to 0.91, amplifying 2-16 alleles. Genetic similarity indices were estimated using the 'NTSYS-pc' software package. Unweighted pair group with arithmetic mean and principal component analysis resolved taxonomic relationships of the aphid species studied. The new aphid microsatellites developed will provide valuable information to researchers to study Indian aphid species diversity and genetic relationships.

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“…The mealybug specimens of each cross were genotyped for six microsatellites developed earlier for P . citri [26]. …”

Section: Methodsmentioning

confidence: 99%

“…Tissue DNA Isolation kit (Omega) following the manufacturer’s protocol. Amplification of microsatellite loci was performed as detailed in [26] using the M13-tailed primer protocol for fluorescence labelling of PCR fragments [29]. Microsatellites were genotyped in an ABI PRISM 310 Genetic Analyser (Applied Biosystems) with GeneScan Rox Size Standard (Applied Biosystems) as internal size standard.…”

Section: Methodsmentioning

confidence: 99%

“…We explored this approach, based on the microsatellites recently developed for P . citri [26], aiming to test the hypothesis of polyandry in mealybugs, suggested in recent behavioral studies, by providing genetic evidence of multiple paternal contribution to the progeny of multiply-mated females. Genetic evidence that mealybug males only contribute half of their genetic material to the progeny, since their haploid set of chromosomes of paternal origin is expected to be eliminated during spermatogenesis, is also presented.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Evidence of Polyandry in the Citrus Mealybug, Planococcus citri (Hemiptera: Pseudococcidae)

Seabra

Brás

Zina³

et al. 2013

PLoS ONE

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The occurrence of polyandry in Planococcus citri , presumed by earlier observations of mating behavior, was confirmed using microsatellite genotyping of pools of over 400 eggs resulting from controlled crosses of one female with two males. The genetic contribution of both mated males was confirmed in 13 out of 43 crosses. In three crosses it was possible to determine that only the first male fertilized the eggs, which may be due to sperm competition or unviable sperm supply. The microsatellite analysis also allowed the confirmation of aspects of the chromosomal inheritance detected previously in cytogenetic studies in Planococcus citri , namely that only one of the alleles is transmitted by the male, indicating that the males are functionally haploid, supporting the observation of Paternal Genome Elimination (PGE) in these insects.

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Isolation and characterization of fifteen polymorphic microsatellite loci for the citrus mealybug, Planococcus citri (Hemiptera: Pseudococcidae), and cross-amplification in two other mealybug species

Cited by 10 publications

References 15 publications

Microsatellite Markers forAleurocanthus spiniferus(Hemiptera: Aleyrodidae) and Their Potential use in Whiteflies

Microsatellite Markers forAleurocanthus spiniferus(Hemiptera: Aleyrodidae) and Their Potential use in Whiteflies

In silico mining of microsatellites and analysis of genetic diversity among inter‐ and intra‐generic aphids of the subfamily Aphidinae

Molecular Evidence of Polyandry in the Citrus Mealybug, Planococcus citri (Hemiptera: Pseudococcidae)

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