Isolation and characterization of galactomannan from seed of Leucaena leucocephala

Rahim, Nurnadiah; Li, Abd Rashid; Kamarun, Dzaraini; Ahmad, Mohd Radzi

doi:10.1007/s00289-017-2135-7

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…The band observed between 1,150 and 946 cm −1 in TWSP and the peaks at 1112–1057 cm −1 in PWSP indicated the presence of C–O and C–O–H bonds shows the presence of glycosidic linkages in the structure of polysaccharides. The peaks present at 897 cm −1 in TWSP was referred as α ‐ d ‐galactopyranosyl and in PWSP, the peaks at 800 and 880 cm −1 were assigned to an anomeric configuration of β ‐ d ‐mannopyranosyl and α ‐ d ‐galactopyranosyl units, respectively (Chawananorasest, Saengtongdee, & Kaemchantuek, ; Rahim et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…The column was subsequently eluted at a flow rate of 0.5 ml/min with deionized water, 0.5 M NaCl and 1 M NaCl, respectively. Clear eluents were then collected and concentrated in vacuum using rotary evaporator (Rahim, Li, Kamarun, & Ahmad, ). The pure water‐soluble TWSP and PWSP were obtained as a precipitate by the addition of ethanol (purity 99.8%) at a ratio of 1:3 (v/v), which was further centrifuged and lyophilized to yield the pure polysaccharide.…”

Section: Methodsmentioning

confidence: 99%

“…represent the characteristic peak of bound water (Zha et al, 2018). The band observed between 1,150 and 946 cm −1 in TWSP and the peaks at galactopyranosyl units, respectively (Chawananorasest, Saengtongdee, & Kaemchantuek, 2016;Rahim et al, 2018).…”

Section: Infrared Spectroscopy Of Isolated Polysaccharidementioning

confidence: 96%

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Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Sivakumar

Kavitha

2019

J Food Process Engineering

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Water‐soluble polysaccharides (WSP) were secluded from Tamarindus indica (TWSP) and Pithecellobium dulce (PWSP) seeds by hot water method. The physico‐chemical properties revealed that the polysaccharides might be used as a potential food additive. The micrometric properties of TWSP and PWSP possessed a bulk density of 0.64 and 0.68 g/ml, respectively. The isolated polysaccharides were subjected to Fourier transform infrared spectroscopy and Nuclear magnetic resonance spectroscopy for its compositional analysis and exhibited that both the isolates are heteropolysaccharides. The spectra of both the mucilages were resonating at δ 3.00–δ 5.00 ppm which confirmed the presence of polysaccharides. Thermo gravimetric analysis showed that the isolated polysaccharides were thermally stable. Scanning electron microscopy and X‐ray diffraction depicted the morphological characteristics. The radical scavenging ability and antibacterial activity proved that the polysaccharides can be explored as a functional food ingredient. Furthermore, the prebiotic potential of polysaccharides stimulated the growth of Lactobacillus delbrueckii and Lactobacillus fermentum over inulin. Practical applications The study provides optimum extraction conditions to obtain TWSP and PWSP seeds using response surface methodology. The isolated polysaccharides can be used as therapeutic agent and as a food additive due to its high purity obtained from Fourier transform infrared spectroscopy and Nuclear magnetic resonance spectroscopic analysis. The excellent flowability and compressibility of polysaccharides can be applied in pharmaceutical formulation as an excipient. The active component present in the isolated polysaccharides can be used as a food preservative. The supplement of TWSP and PWSP as a carbon source accelerated the growth of probiotics which could be used as natural prebiotics. Thus the study concluded that the water‐soluble polysaccharide obtained from the agro‐industrial by product has the wide application in food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Sivakumar

Kavitha

2019

J Food Process Engineering

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“…For a better comparison, figure 6 shows the FT-IR spectra of the prepared galactomannan/Zn(OH) 2 -ZnO composite and the extracted and purified galactomannan as a reference (the same that was used for preparing the composite material, and whose main characteristics were reported previously [26]). For the reference case, it is observed that the FT-IR spectrum shows the characteristic bands usually reported in the literature for galactomannan polysaccharides [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52]. In this way, the broad band at around 3360 cm −1 observed in the reference spectrum corresponds to the O-H stretching vibrations of the hydroxymethyl (-CH 2 OH) The FT-IR spectrum of the galactomannan reference also presents two bands at 1739 and 1645 cm −1 .…”

Section: 1c Ft-ir Spectroscopy Characterizationmentioning

confidence: 93%

Characterization of the antibacterial galactomannan/ $$\hbox {Zn(OH)}_{2}$$ Zn(OH) 2 –ZnO composite material prepared in situ from a green process using mesquite seeds as a biopolymer source

et al. 2019

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In this work, the preparation of an antibacterial galactomannan/Zn(OH) 2-ZnO composite material by a green process is proposed and demonstrated. The galactomannan polysaccharide was extracted from the arid-native mesquite (Prosopis spp.) seeds through a hydrothermal technique, and it was used as an organic polymeric matrix for the in situ chemical precipitation of the Zn(OH) 2-ZnO inorganic particles from simple reactions between Zn 2+ and NaOH. The preparation was performed under standard conditions in aqueous solutions. The composite material, which was obtained by centrifugation of the previous preparation, was characterized by X-ray diffraction, transmission and scanning electron microscopy, infrared spectroscopy and thermal analysis. By means of these techniques, it was deduced that the composite material is highly crystalline (about 94%), composed of about 92% inorganic and 8% organic portion, and has a predominant size of 760 nm and a mean size of 800 nm. The antibacterial activity of the composite material was also studied, which resulted better against Escherichia coli (Gram-negative bacterium) than against Staphylococcus aureus (Gram-positive bacterium), which was calculated in a difference of about 30%. This work presents the high potential of galactomannans to form functional composite materials with inorganic particles.

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“…The desired property was then investigated utilizing the standard calibration curve (molecular weight of dextran standards versus. elution times) (Rahim et al, 2018).…”

Section: Gel Permeation Chromatography (Gpc)mentioning

confidence: 99%

A new source of galactomannan isolated from Gleditsia caspica (Persian honey locust) seeds: Extraction and comprehensive characterization

Niknam

Mousavi

Kiani

2021

J. Food Process. Preserv.

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The dominant objective of this study was to extract and purify galactomannan from Gleditsia caspica seeds. In addition to chemical analysis and investigation of the basic physicochemical and functional properties of the extracted galactomannan, other tests have been performed to determine the structural and thermal properties. The FT-IR spectra authenticated availability of O-H, C-H, and C-O stretching vibrations in the galactomannan conformation. The extracted galactomannan had amorphous structure with very low crystallinity which was obtained from XRD analysis. The obtained DSC and TGA plots showed thermal events including one endothermic and one exothermic peak that are typical for natural polysaccharides. The H-NMR analysis of the extracted galactomannan demonstrated peaks at δ 4.901 and 4.705 ppm, which were affiliated with availability of H-1 (α) (α-galactopyranosyl) and H-2 (β) (β-mannopyranosyl). The images obtained from SEM confirmed that the galactomannan surface was relatively smooth and uniform. Novelty Impact StatementThe galactomannan was extracted and purified from Gleditsia caspica seeds.Comprehensive characterization of obtained galactomannan authenticated its chance to be applied as thickeners or stabilizers in food formulations as a new source of plant-based hydrocolloid.

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Isolation and characterization of galactomannan from seed of Leucaena leucocephala

Cited by 14 publications

References 23 publications

Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Characterization of the antibacterial galactomannan/ $$\hbox {Zn(OH)}_{2}$$ Zn(OH) 2 –ZnO composite material prepared in situ from a green process using mesquite seeds as a biopolymer source

A new source of galactomannan isolated from Gleditsia caspica (Persian honey locust) seeds: Extraction and comprehensive characterization

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