Isolation and characterization of heavy-metal-resistant bacteria and their applications in environmental bioremediation

Kalaimurugan, Dharman; Balasubramanian, Balamuralikrishnan; Durairaj, Kaliannan; Vasudhevan, Palanisamy; Shivakumar, Muthugounder Subramanian; Kaul, Tanushri; Chang, Soon Woong; Ravindran, Balasubramani; Venkatesan, S.

doi:10.1007/s13762-019-02563-5

Cited by 63 publications

(22 citation statements)

References 36 publications

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“…The poly- p -xylylene fabrication technology can constitute a defined inner porous structure to capture different size of organic particles to further concentrate organic matters and a controllable size and shape of the products to fit to different situations. Until now, most of the present studies still focused on identifying the bacteria with specific enzyme functions to use in the bioremediation process [ 5 , 25 , 26 ]. We foresee the application of capsule products to the degradation of organic compounds, wastewater, and environmental pollution, as well as the removal of potential hazardous chemicals.…”

Section: Discussionmentioning

confidence: 99%

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Yang

Huang

et al. 2020

Polymers

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A regulatable bioremediation capsule material was synthesized with isolated single-strain bacteria (Bacillus species, B. CMC1) and a regulator molecule (carboxymethyl cellulose, CMC) by a vapor-phased encapsulation method with simple steps of water sublimation and poly-p-xylylene deposition in chemical vapor deposition (CVD) process. Mechanically, the capsule construct exhibited a controllable shape and dimensions, and was composed of highly biocompatible poly-p-xylylene as the matrix with homogeneously distributed bacteria and CMC molecules. Versatility of the encapsulation of the molecules at the desired concentrations was achieved in the vapor-phased sublimation and deposition fabrication process. The discovery of the fabricated capsule revealed that viable living B. CMC1 inhabited the capsule, and the capsule enhanced bacterial growth due to the materials and process used. Biologically, the encapsulated B. CMC1 demonstrated viable and functional enzyme activity for cellulase activation, and such activity was regulatable and proportional to the concentration of the decorated CMC molecules in the same capsule construct. Impressively, 13% of cellulase activity increase was realized by encapsulation of B. CMC1 by poly-p-xylylene, and a further 34% of cellulase activity increase was achieved by encapsulation of additional 2.5% CMC. Accordingly, this synergistic effectiveness of the capsule constructs was established by combining enzymatic B. CMC1 bacteria and its regulatory CMC by poly-p-xylylene encapsulation process. This reported encapsulation process exhibited other advantages, including the use of simple steps and a dry and clean process free of harmful chemicals; most importantly, the process is scalable for mass production. The present study represents a novel method to fabricate bacteria-encapsulated capsule for cellulose degradation in bioremediation that can be used in various applications, such as wastewater treatment and transforming of cellulose into glucose for biofuel production. Moreover, the concept of this vapor-phased encapsulation technology can be correspondingly used to encapsulate multiple bacteria and regulators to enhance the specific enzyme functions for degradation of various organic matters.

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Section: Discussionmentioning

confidence: 99%

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Yang

Huang

et al. 2020

Polymers

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“…Many PGPR strains capable of removing pollutants from environmental media are found in the rhizosphere soils [98]. dos Santos and Maranho [33] reported the efficiency of Bacillus, Alcaligenes, Microbacterium, and Curtobacterium isolated from rhizosphere soil in heavy metal transformation.…”

Section: The Role Of Rhizosphere Soil and Its Bacteria For Bioremediation And Biofiltrationmentioning

confidence: 99%

Elucidating the Rhizosphere Associated Bacteria for Environmental Sustainability

Nwachukwu

Babalola

2021

Agriculture

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The abundance of nutrient accumulation in rhizosphere soils has placed the rhizosphere as an “epicenter” of bacterial concentrations. Nonetheless, over the years, little attention has been given to bacterial inoculants and soil-like substrates. The reason is that many farmers and experiments have focused on chemical fertilizers as an approach to improve plant growth and yield. Therefore, we focused on assessing the application of rhizosphere soil and its associated bacteria for biotechnological applications. This review has been structured into major subunits: rhizosphere soil as a treasure trove for bacterial community concentration, biodegradation of lignocellulose for biofuel production, rhizosphere soil and its bacteria as soil amendments, and the role of rhizosphere soil and its bacteria for bioremediation and biofiltration. Hence, the efficient use of rhizosphere soil and its bacteria in an environmentally friendly way can contribute to healthy and sustainable environments.

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“…This process is used for removing metal or metalloid species, compounds and particulates from solutions by biological materials [12,40]. The most important types of biosorbents that have been developed from diverse raw biomass are bacteria due to their ubiquity, size, ability to grow under controlled conditions and resilience to environmental conditions (e.g., Pseudomonas fluorescens; Bacillus safensis; Pseudomonas aeruginosa) [41,42]. L. fusiformis is able to adapt and survive under extremely stressful conditions.…”

Section: Determination Of Heavy Metal Content Using Atomic Absorption Spectrometry (Aas) Techniquementioning

confidence: 99%

“…The remediation is carried out in the site of contamination by stimulating the growth of indigenous microbes, supplying adequate nutrition or application of engineered microbes [65]. Therefore, many organisms have been used from different raw biomass, such as bacteria (e.g., Pseudomonas fluorescens; Bacillus safensis; Pseudomonas aeruginosa; Bacillus thuringiensis) [41,42,66], fungi (e.g., Botrytis cinerae) [12,67] and algae (e.g., Anabaena sphaerica) [68]. However, there are several advantages of microbialbased biosorption in the removal of metal ions, such as the high metal removal efficiency thanks to their selectivity towards particular metal [64].…”

Section: Bio-treatment Assays Of Phosphate Laundries Wastewater Using Lysinibacillus Fusiformismentioning

confidence: 99%

Isolation, Characterization and Identification of a New Lysinibacillus fusiformis Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays

et al. 2021

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The aim of the present study is to isolate, characterize and identify a novel strain ZC from the Metlaoui phosphate laundries wastewater (MPLW). The chemical characterization of this phosphate rich effluent showed an alkaline pH and is saline, highly turbid and rich in suspended matter and total solids. The MPLW samples were loaded with potentially toxic metals, presented in decreasing order as follows: magnesium (5655 mg L−1), potassium (45 mg L−1), lead (1 mg L−1), iron (0.7 mg L−1), cadmium (0.5 mg L−1), copper (0.3 mg L−1) and zinc (0.1 mg L−1). Due to the high COD/BOD5 ratio, a poorly biodegradable organic load is underlining. The newly isolated strain was identified as Lysinibacillus fusiformis using 16S rDNA sequencing analysis. The viability of this new strain was tested in presence of the zinc, lead, cadmium, manganese and copper at 1, 10 and 100 mM. The L. fusiformis survival, under metallic stress, was inversely proportional to metal ion concentrations, while lead and zinc were the most toxic ones using MTT assay. Then, the newly isolated strain was characterized in terms of enzyme production, proteomic alteration and antibiotic resistance. The strain ZC revealed some modifications in the biochemical and enzymatic profiles by either the appearance or/and the disappearance of some activities. In addition, the increase in metal ions stress and concentrations was proportional to the adherence and to the hydrophobicity. The presence of the metal ions suggested the change of sensitivity to the resistance of this strain towards tobramycin, kanamycin, neomycin, netilmicin and cefoxitin, showing an increase in the MARindex. The strain ZC, used as a biological tool for MPLW treatment, showed a reduction in the metal ion contents. This reduction was due to accumulation and/or adsorption, showing a bioprocessing performance of the newly isolated L. fusiformis.

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Isolation and characterization of heavy-metal-resistant bacteria and their applications in environmental bioremediation

Cited by 63 publications

References 36 publications

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Synergistic and Regulatable Bioremediation Capsules Fabrication Based on Vapor-Phased Encapsulation of Bacillus Bacteria and its Regulator by Poly-p-Xylylene

Elucidating the Rhizosphere Associated Bacteria for Environmental Sustainability

Isolation, Characterization and Identification of a New Lysinibacillus fusiformis Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays

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