Abstract:Background and Aim
H. pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients.
Methods
We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR.
Results
H. pylori were recovered under anaerobic and micro-aerobic conditions from … Show more
“… 59 Our results are in accordance with Perez-Perez et al. 39 who reported that two patients out of the eight CagA positive H. pylori isolates lacked the cagA gene. Fig.…”
Section: Resultssupporting
confidence: 91%
“…Of 20 H. pylori strains, 12 (75%) showed the s1/m2 genotype and four showed the s1/m1 genotype. 39 Some of the H. pylori strains showed multiple alleles, despite single colonies being picked. 39 Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Bacterial isolates recovered from microaerophilic conditions were confirmed phenotypically as H. pylori on the basis of positive reactions for urease, catalase and oxidase tests. 39 In this study 20 out of 45 cultivated gastric biopsies were positive for H. pylori and were designated as HP-1 to HP-20.…”
The Pan-Drug Resistant (PDR),
Helicobacter pylori
remains an intractable challenge in public health worldwide and this pathogenicity is mainly due to the presence of a cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA). On the other hand, plant extracts such as
Syzygium aromaticum
contain a diverse array of secondary metabolites, which could be potentially used to combat
H. pylori
pathogens. To our knowledge, this is the first report on the biomedical potential of
S. aromaticum
extract against cytotoxin-associated genes producing PDR
H. pylori
. In this investigation, out of 45 gastric antral biopsy specimens of dyspeptic patients, 20 strains were confirmed as
H. pylori.
Eight (40%) out of 20 strains were PDR
H. pylori
while the rest of the strains were Multi-Drug Resistant (MDR) strains. Genotypic analyses of PDR
H. pylori
strains showed that
cagA
and
vacA
genes were found to be 75% and 87.5%, respectively and
m2s2
was the most common subtype of
vacA
gene.
S. aromaticum
showed a significant higher anti-
H. pylori
activity compared to that of
Cinnamomum zeylanicum
and
Thymus vulgaris
. Eugenol was the major phenolic compound (28.14%) detected in the methanolic extract of
S. aromaticum
. Clearly, results of the toxicological assessment confirmed the safety of
S. aromaticum
for use. Hence, these results suggest that
S. aromaticum
could be a new useful natural antimicrobial agent that could potentially combat cytotoxin genes-producing drug-resistant
H. pylori
. Moreover, these findings provide a scientific basis for the development of antimicrobial agents from traditional herbal medicines for gastroprotection against gastric ulcer.
“… 59 Our results are in accordance with Perez-Perez et al. 39 who reported that two patients out of the eight CagA positive H. pylori isolates lacked the cagA gene. Fig.…”
Section: Resultssupporting
confidence: 91%
“…Of 20 H. pylori strains, 12 (75%) showed the s1/m2 genotype and four showed the s1/m1 genotype. 39 Some of the H. pylori strains showed multiple alleles, despite single colonies being picked. 39 Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Bacterial isolates recovered from microaerophilic conditions were confirmed phenotypically as H. pylori on the basis of positive reactions for urease, catalase and oxidase tests. 39 In this study 20 out of 45 cultivated gastric biopsies were positive for H. pylori and were designated as HP-1 to HP-20.…”
The Pan-Drug Resistant (PDR),
Helicobacter pylori
remains an intractable challenge in public health worldwide and this pathogenicity is mainly due to the presence of a cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA). On the other hand, plant extracts such as
Syzygium aromaticum
contain a diverse array of secondary metabolites, which could be potentially used to combat
H. pylori
pathogens. To our knowledge, this is the first report on the biomedical potential of
S. aromaticum
extract against cytotoxin-associated genes producing PDR
H. pylori
. In this investigation, out of 45 gastric antral biopsy specimens of dyspeptic patients, 20 strains were confirmed as
H. pylori.
Eight (40%) out of 20 strains were PDR
H. pylori
while the rest of the strains were Multi-Drug Resistant (MDR) strains. Genotypic analyses of PDR
H. pylori
strains showed that
cagA
and
vacA
genes were found to be 75% and 87.5%, respectively and
m2s2
was the most common subtype of
vacA
gene.
S. aromaticum
showed a significant higher anti-
H. pylori
activity compared to that of
Cinnamomum zeylanicum
and
Thymus vulgaris
. Eugenol was the major phenolic compound (28.14%) detected in the methanolic extract of
S. aromaticum
. Clearly, results of the toxicological assessment confirmed the safety of
S. aromaticum
for use. Hence, these results suggest that
S. aromaticum
could be a new useful natural antimicrobial agent that could potentially combat cytotoxin genes-producing drug-resistant
H. pylori
. Moreover, these findings provide a scientific basis for the development of antimicrobial agents from traditional herbal medicines for gastroprotection against gastric ulcer.
“…11 Concerning culture, the novelty was the possibility to grow H. pylori under anaerobic conditions, which indeed surprisingly yielded significantly higher recovery rates than under microaerobic conditions. 12 Along the same lines, a chemolithoautotrophic growth was described for H. pylori. An H 2 containing atmosphere achieved 40-60% greater growth within 16 h than without H 2 .…”
Section: Methods Based On the Performance Of Endoscopymentioning
confidence: 90%
“…Concerning culture, the novelty was the possibility to grow H. pylori under anaerobic conditions, which indeed surprisingly yielded significantly higher recovery rates than under microaerobic conditions …”
Section: Methods Based On the Performance Of Endoscopymentioning
Important progress is being made in endoscopic methods which allow clinicians to predict the presence of Helicobacter pylori based on characteristics of gastric mucosa and to obtain targeted biopsies. There are also important developments in molecular methods with various techniques derived from standard PCR, applied both on gastric biopsies and stool specimens. Progress is being made in microfluidic systems to get a reliable diagnosis in a very short time. The interest of the C urea breath test has been confirmed as well as stool antigen tests. Attempts are being made to find biological markers of premalignant conditions by serology, other than pepsinogens.
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