1994
DOI: 10.1111/j.1432-1033.1994.tb18575.x
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Isolation and characterization of hirustasin, an antistasin‐type serine‐proteinase inhibitor from the medical leech Hirudo medicinalis

Abstract: Antistasin, a potent inhibitor of the blood coagulation factor Xa, is the prototype of a novel family of serine-proteinase inhibitors. We have now isolated, sequenced and characterized an antistasin-type inhibitor from the medical leech Hirudo medicinalis. Hirustasin (Hirudo antistasin) was purified to apparent homogeneity by cation-exchange and affinity chromatography. Amino acid sequencing of the 55 amino acid protein (MI 5866) revealed that hirustasin is the only antistasintype protein known to consist of o… Show more

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Cited by 76 publications
(67 citation statements)
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“…The specificity of vesicle fusion is determined primarily by the ability of organelles/vesicles to recognize each other through molecules referred to as soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptors (SNAREs). SNAREs on one class of vesicles are hypothesized to recognize and form complexes with their cognate receptors, SNAREs on other vesicles (Sollner et al, 1993(Sollner et al, , 1994Ferro-Novick and Jahn, 1994;Rothman and Warren, 1994). SNAREs responsible for specific organelle fusion events have been identified for both the endocytic and secretory pathways.…”
Section: Introductionmentioning
confidence: 99%
“…The specificity of vesicle fusion is determined primarily by the ability of organelles/vesicles to recognize each other through molecules referred to as soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptors (SNAREs). SNAREs on one class of vesicles are hypothesized to recognize and form complexes with their cognate receptors, SNAREs on other vesicles (Sollner et al, 1993(Sollner et al, , 1994Ferro-Novick and Jahn, 1994;Rothman and Warren, 1994). SNAREs responsible for specific organelle fusion events have been identified for both the endocytic and secretory pathways.…”
Section: Introductionmentioning
confidence: 99%
“…These include thrombin inhibitors such as hirudin (6) and haemadin (7) and coagulation factor Xa inhibitors such as antistasin (8) and ghilanten (9). The second group of inhibitors acts on the extracellular matrix; these include, for example, bdellins (10), eglins (11), gelin (12), guamerins (13,14), and hirustasin (15).…”
mentioning
confidence: 99%
“…A single zone at a with molecules of the antistasin-type inhibitors family i.e. antistasin [24], ghilanthen [7], hydra antistasin [25], hirustasin [11], retention time between 27Ϫ30 min corresponding to proteins having a molecular mass less than 14.4 kDa, was detected. The guamerin and guamerin II [26,27] (Fig.…”
mentioning
confidence: 99%
“…eight cysteines residues engaged in four disulfide bridges (5378 Da). In regard to known tryspin-chymotrypsin inhibitors isolated RESULTS AND DISCUSSION from jawed leeches (Hirudo medicinalis and Hirudo manillensis), i.e., bdellins [9], gelin [10] and eglin [11], no sePurification of therin. The results of the stepwise purification of therin are summarized in Table 1.…”
mentioning
confidence: 99%