Isolation and characterization of human intestinal <i>Enterococcus avium</i>
 EFEL009 converting rutin to quercetin

Shin, Na Rae; Moon, Junghwan; Shin, Sera; Li, Ling; Lee, Yoon Bok; Kim, Tae-Jip; Han, Nam Soo

doi:10.1111/lam.12512

Cited by 48 publications

(26 citation statements)

References 25 publications

(35 reference statements)

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“…The production of quercetin by bacteria commonly found in food, as lactic acid bacteria and bifidobacteria, has been described in two Enterococcus strains (Schneider et al , ; Shin et al , ). In our work, just two B. breve and two B. pseudocatenulatum were able to produce quercetin, although a decrease on the glycoside isoquercetin was observed also for other Bifidobacterium strains.…”

Section: Discussionmentioning

confidence: 99%

Production of flavonoid and lignan aglycones from flaxseed and soy extracts by Bifidobacterium strains

Peirotén

Álvarez

Landete

2019

Int J of Food Sci Tech

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Flaxseed and soybean are an important source of phytochemical compounds, mainly lignans and isoflavones, but also of flavones, flavanones and flavonols. These compounds appear mainly glycosylated in plant food and are converted into aglycones by the intestinal microbiota, increasing their bioavailability. In this work, we analyse the ability of Bifidobacterium strains (n = 25) to produce lignan and flavonoids aglycones from flaxseed and soybean extracts. Most of the Bifidobacterium strains increased the concentrations of secoisolariciresinol, daidzein, genistein, naringenin, eriodyctiol, luteolin and apigenin. Moreover, Bifidobacterium pseudocatenulatum and Bifidobacterium breve strains showed high production of herbacetin, increased the kaempferol concentration and produced quercetin and quercetagetin. B. pseudocatenulatum INIA P815 and B. breve INIA P367 produced 32.37 AE 2.81 and 28.64 AE 3.36 mg respectively of herbacetin g À1 of lignan extract. Bifidobacterium strains transformed the glycosides of a wide range of flavonoids into their aglycones, increasing the antioxidant activity and improving their bioavailability. Metabolism of flavonoids by bifidobacteria A. Peirot en et al. 2123 102.6 AE 57.6 139.4 AE 32.4 141.5 AE 21.7 B. pseudocatenulatum INIA P846 152.8 AE 22.1 158.4 AE 12.0 168.2 AE 18.8 B. pseudocatenulatum INIA P946 204.2 AE 35.3 154.8 AE 6.4 195.7 AE 11.1 B. pseudolongum INIA P2 13.6 AE 4.4 65.4 AE 2.3 73.2 AE 15.2 B. pseudolongum INIA P120 16.8 AE 7.5 46.7 AE 2.6 69.5 AE 11.7 B. pseudolongum INIA P977 15.8 AE 4.4 62.1 AE 16.9 101.6 AE 8.0 Metabolism of flavonoids by bifidobacteria A. Peirot en et al. †Units are expressed as equivalents of Trolox or Gallic acid expressed as mg mL À1 of fermentation media. Metabolism of flavonoids by bifidobacteriaA. Peirot en et al.

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Section: Discussionmentioning

confidence: 99%

Production of flavonoid and lignan aglycones from flaxseed and soy extracts by Bifidobacterium strains

Peirotén

Álvarez

Landete

2019

Int J of Food Sci Tech

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“…Other bioavailability studies report plasma quercetin concentration from 50 nM up to 1.6 µM depending if subjects were consuming habitual diets, quercetin rich diets or the pure compound [3]. Quercetin is rapidly absorbed in the proximal parts of the gastrointestinal tract, whereas absorption of rutin occurs in the distal parts and is slow, since it must be hydrolyzed by the colonic microflora [3,4,63,64]. Like other flavonoids quercetin almost exclusively appears in the serum as glucuronide and sulfate conjugates, which have been shown to retain their antioxidant properties and which are slowly eliminated (half-lives from 11 to 28 hrs have been reported) so that repeated intake of rutin and quercetin-containing diet might lead to accumulation of metabolites in blood [3,[63][64][65][66].…”

Section: Discussionmentioning

confidence: 99%

“…It is the aglycone of a number of flavonoid glycosides including rutin [3]. In the gut quercetin is formed through hydrolysis of rutin by intestinal microorganisms [4]. The pharmacological actions of rutin and quercetin include inhibition of lipopolysaccharide-induced nitric oxide production, antioxidant, anti-hyperglycemic, anti-inflammatory, cytoprotective, hepatoprotective and chemopreventive activities [5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells

Kittl

Beyreis

Tumurkhuu

et al. 2016

Cell Physiol Biochem

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Background/Aims: Previously we described insulinotropic effects of Leonurus sibiricus L. plant extracts used for diabetes mellitus treatment in Traditional Mongolian Medicine. The flavonoid quercetin and its glycoside rutin, which exert anti-diabetic properties in vivo by interfering with insulin signaling in peripheral target tissues, are constituents of these extracts. This study was performed to better understand short- and long-term effects of quercetin and rutin on beta-cells. Methods: Cell viability, apoptosis, phospho-protein abundance and insulin release were determined using resazurin, annexin-V binding assays, Western blot and ELISA, respectively. Membrane potentials (V_mem), whole-cell Ca²⁺ (ICa)- and ATP-sensitive K⁺ (IK_ATP) currents were measured by patch clamp. Intracellular Ca²⁺ (Ca_i) levels were measured by time-lapse imaging using the ratiometric Ca²⁺ indicator Fura-2. Results: Rutin, quercetin and the phosphoinositide-3-kinase (PI3K) inhibitor LY294002 caused a dose-dependent reduction in cell viability with IC₅₀ values of ∼75 µM, ∼25 µM and ∼3.5 µM, respectively. Quercetin (50 µM) significantly increased the percentage of Annexin-V+ cells within 48 hrs. The mean cell volume (MCV) of quercetin-treated cells was significantly lower. Within 2 hrs, quercetin significantly decreased basal- and insulin-stimulated Akt(T308) phosphorylation and increased Erk1/2 phosphorylation, without affecting P-Akt(S473) abundance. Basal- and glucose-stimulated insulin release were significantly stimulated by quercetin. Quercetin significantly depolarized V_mem by ∼25 mV which was prevented by the K_ATP-channel opener diazoxide, but not by the L-type ICa inhibitor nifedipine. Quercetin significantly stimulated ICa and caused a 50% inhibition of IK_ATP. The effects on V_mem, ICa and IK_ATP rapidly reached peak values and then gradually diminished to control values within ∼1 minute. With a similar time-response quercetin induced an elevation in Ca_i which was completely abolished in the absence of Ca²⁺ in the bath solution. Rutin (50 µM) did not significantly alter the percentage of Annexin-V+ cells, MCV, Akt or Erk1/2 phosphorylation, insulin secretion, or the electrophysiological behavior of INS-1 cells. Conclusion: We conclude that quercetin acutely stimulates insulin release, presumably by transient K_ATP channel inhibition and ICa stimulation. Long term application of quercetin inhibits cell proliferation and induces apoptosis, most likely by inhibition of PI3K/Akt signaling.

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“…[ 12,14 ] Most of the flavonoids in foods occur as glycosides, mainly O ‐glycosides but also C ‐glycosides ( Table 1 ). [ 14–42 ] Exceptions to this are flavan‐3‐ols, which are not conjugated but can form oligomeric and polymeric structures called proanthocyanidins or condensed tannins as a whole or procyanidins, prodelphinidins or propelargonidins when they are only composed of (epi)catechin, (epi)gallocatechin or (epi)azfelechin, respectively. Gut microbiota is able to breakdown the flavonoid C‐ ring in different positions, releasing a higher number of simple phenolics derived from A and B rings.…”

Section: The Two‐way Interaction Between Phenolics and Gut Microbiotamentioning

confidence: 99%

Where to Look into the Puzzle of Polyphenols and Health? The Postbiotics and Gut Microbiota Associated with Human Metabotypes

Cortés‐Martín

Selma

Tomás‐Barberán

et al. 2020

Molecular Nutrition Food Res

199

240

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The full consensus on the role of dietary polyphenols as human-healthpromoting compounds remains elusive. The two-way interaction between polyphenols and gut microbiota (GM) (i.e., modulation of GM by polyphenols and their catabolism by the GM) is determinant in polyphenols' effects. The identification of human metabotypes associated with a differential gut microbial metabolism of polyphenols has opened new research scenarios to explain the inter-individual variability upon polyphenols consumption. The metabotypes unequivocally identified so far are those involved in the metabolism of isoflavones (equol and(or) O-desmethylangolesin producers versus non-producers) and ellagic acid (urolithin metabotypes, including producers of only urolithin-A (UM-A), producers of urolithin-A, isourolithin-A, and urolithin-B (UM-B), and non-producers (UM-0)). In addition, the microbial metabolites (phenolic-derived postbiotics) such as equol, urolithins, valerolactones, enterolactone, and enterodiol, and 8-prenylnaringenin, among others, can exert differential health effects. The knowledge is updated and position is taken here on i) the two-way interaction between GM and polyphenols, ii) the evidence between phenolic-derived postbiotics and health, iii) the role of metabotypes as biomarkers of GM and the clustering of individuals depending on their metabotypes (metabotyping) to explain polyphenols' effects, and iv) the gut microbial metabolism of catecholamines to illustrate the intersection between personalized nutrition and precision medicine.A. Cortés-Martín, Dr.

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Isolation and characterization of human intestinal Enterococcus avium EFEL009 converting rutin to quercetin

Cited by 48 publications

References 25 publications

Production of flavonoid and lignan aglycones from flaxseed and soy extracts by Bifidobacterium strains

Production of flavonoid and lignan aglycones from flaxseed and soy extracts by Bifidobacterium strains

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells

Where to Look into the Puzzle of Polyphenols and Health? The Postbiotics and Gut Microbiota Associated with Human Metabotypes

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