Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations

Park, Jung Chul; Kim, Jeong‐Min; Jung, In Chul; Kim, Jane C.; Choi, Seong‐Ho; Cho, Kyoo-Sung; Kim, Chang Sung

doi:10.1111/j.1600-051x.2011.01716.x

Cited by 244 publications

(292 citation statements)

References 34 publications

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“…Due to the experimental perforation of the floor of the pulp chamber in mice, growth of chronic inflammatory granulation tissue was found to be the main tissue change without causing purulent inflammation. Recently, several studies mentioned that the cellular components of the granulation tissue were derived from the periodontal ligament stem cells (PDLSCs) [9][10][11] . We utilized bone marrow transplanted GFP mouse to analyze cell differentiation and migration in periodontal tissue.…”

Section: Discussionmentioning

confidence: 99%

Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

Matsuda

Yokoi

Moriyama

et al. 2015

J. Hard Tissue Biology.

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: The mechanism in the formation of periodontal polyp has been established in several histological studies but details on cell differentiation and/or proliferation have not been elucidated. In the present study, we established a convenient and possible experimental system using ddY mice. Briefly, pentobarbital sodium (Somnopentyl) was injected into the abdominal cavity of the mouse followed by access cavity preparation on maxillary first molar using low speed ½ round bur (Merufa Inc), exposing the pulp and then allowed to perforate the floor of the pulp chamber. Observation was done over time until 6 months using micro CT (m_CT) image photography. Results with transmission image using m_CT showed the expansion in the width of the periodontal ligament in the furcation area. The lesion was excised as one mass and examined histopathologically. The granulation tissue was covered with stratified squamous epithelium. The present experimental technique has been confirmed to be effective in analyzing the formation of periodontal polyp induced by mechanical perforation.

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Section: Discussionmentioning

confidence: 99%

Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

Matsuda

Yokoi

Moriyama

et al. 2015

J. Hard Tissue Biology.

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“…3,5 It has also been suggested that stem cells from an inflamed PDL still retain selfrenewal and differentiation capacities. 6 PDL cells were found to differentiate into cementoblast-like cells, osteoblast-like cells, adipocytes, and collagenforming cells in vitro, and transformed into cementum/PDL-like structures when they were transplanted with a calcium HA scaffold into immunocompromised rodents. 7 Collagen fibers from transplanted PDLSCs were able to link with the cementoblast-like structures which resemble the physiological attachment of Shapey's fibers.…”

Section: Discussion Of Findings and Relevant Literaturementioning

confidence: 99%

Application of Stem Cell Technology in Dental Regenerative Medicine

Feng

Lengner

2013

Advances in Wound Care

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“…[2] These cells appear localized around and adjacent to blood vessels in the PDL space, and McCulloch identifi ed them as progenitor cells. [3] These cells exhibits the characteristic smaller size than the adjacent cells, high nuclear/cytoplasmic ratio and slow cell division.…”

Section: Historymentioning

confidence: 99%

“…Extraoral sites may be bone marrow mesenchymal stem cells and adipose-derived stem cells. [2] More than any other tissue, the complexity of the periodontium along with the understanding that only certain components are capable of achieving regeneration, makes the identifi cation of stem cells important, not only in the understanding of normal wound healing events but also in designing novel clinical techniques. Seo et al [5] isolated the multipotent human PDL stem cells by single-colony selection and magnetic-activated cell sorting, and characterized according to their expression of the mesenchymal stem cell markers STRO-1 and CD 146/MUC18.…”

Section: Historymentioning

confidence: 99%

Periodontal ligament stem cell: An update

Jain¹,

Agarwal²,

Gokhale³

et al. 2014

JCRI

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The periodontium is a complex of four tissues of which two are hard and rests are soft. The hard tissue being the alveolar bone and the cementum and soft tissue consists of the periodontal ligament and the gingiva. Periodontitis is multifactorial infectious disease of the supporting structures of the teeth, characterized by destruction of the bone and connective tissue. The ability to identify and manipulate stem cells has been the basis of tissue engineering based clinical therapies. This has been successfully applied in the fi eld of regenerative medicine. However, its successful application in the fi eld of dentistry for periodontal regeneration is still in its infancy. Novel techniques such as tissue engineering need to be developed to counter to diffi culties associated with achieving predictable periodontal regeneration. However, stem cells can be a promising option in this regard. Thus, this review will explore the immense potential and the contemporary concept of stem cells; also it will highlight the recent developments in identifi cation and clinically implication of these cells.

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Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations

Abstract: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.

Cited by 244 publications

References 34 publications

Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

Application of Stem Cell Technology in Dental Regenerative Medicine

Periodontal ligament stem cell: An update

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