Isolation and characterization of
 Nocardia seriolae
 , a causative agent of systematic granuloma in cultured East Asian four finger threadfin,
 Eleutheronema rhadinum,
 and red snapper,
 Lutjanus erythropterus

Liao, Pei-Chih; Tsai, Ming‐An; See, Ming She; Wang, Pei‐Chi; Chen, Shih‐Chu

doi:10.1111/are.14932

Cited by 13 publications

(28 citation statements)

References 27 publications

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“…N. seriolae infections have a significant impact on fish farming and food security in Asia, with nocardiosis outbreaks continuing to result in large economic losses due to mass fish mortalities (Labrie et al, 2008, Maekawa et al, 2018, Lei et al, 2020, Liao et al, 2021. Conventional N. seriolae genotyping methods e.g.…”

Section: Discussionmentioning

confidence: 99%

Simple and cost-effective SNP genotyping method for discriminating subpopulations of the fish pathogen, Nocardia seriolae

Prince

Sarovich

et al. 2021

Preprint

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Nocardia seriolae has caused significant fish losses in Asia and the Americas in recent decades, including in Vietnam, which has witnessed devastating economic and social impacts due to this bacterial pathogen. Surveillance strategies are urgently needed to mitigate N. seriolae dissemination in Vietnamese aquaculture and mariculture industries. Whole-genome sequencing (WGS) offers the highest level of resolution to discriminate closely related strains and to determine their putative origin and transmission routes. However, WGS is impractical for epidemiological investigations and pathogen surveillance due to its time-consuming and costly nature, putting this technology out-of-reach for many industry end-users. To overcome this issue, we targeted two previously characterised, phylogenetically informative single-nucleotide polymorphisms (SNPs) in N. seriolae that accurately distinguish: i) Vietnamese from non-Vietnamese strains, and ii) the two Vietnamese subclades. Using the mismatch amplification mutation assay (MAMA) format, we developed assays that genotype strains based on differences in amplicon melting temperature (melt-MAMA) and size (agarose-MAMA). Our MAMA assays accurately genotyped strains both from culture and fish tissues at low cost, using either real-time (~AUD$1/per sample) or conventional (~AUD$0.50/per sample) PCR instrumentation. Our novel assays provide a rapid, reproducible, and cost-effective tool for routine genotyping of this pathogen, allowing faster identification and treatment of nocardiosis-effected permit fish within Vietnamese aquaculture/mariculture facilities, an essential step in mitigating N. seriolae-associated losses.

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Section: Discussionmentioning

confidence: 99%

Simple and cost-effective SNP genotyping method for discriminating subpopulations of the fish pathogen, Nocardia seriolae

Prince

Sarovich

et al. 2021

Preprint

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“…Clinical signs Sluggish swimming, emaciation, fin and skin hyperaemia and anorexia skin lesion, necrosis, skin ulceration, lethargy, pectoral haemorrhages or focal dermal ulcers, exophthalmia, necrotic degeneration, desquamation, infiltration of inflammatory cells, abdominal distention and change in skin colour Wang et al (2007), Kim et al (2018), Liao et al (2021), del Rio-Rodriguez et al (2021, Hoang et al (2021) Histopathological signs…”

Section: Category Description Referencesmentioning

confidence: 99%

“…Granulomas formation in internal organs, including muscles, kidney, spleen, gills, heart, liver and brain Labrie et al (2008), Wang et al (2017a), Brosnahan et al (2017), Liao et al (2021) Nodules formation in tissues due to granulomata formation…”

Section: Category Description Referencesmentioning

confidence: 99%

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Pathogenicity, diagnosis, prevention strategies and immune response of bacterium Nocardia seriolae : A critical review

Nawaz

Gao

Huang

et al. 2022

Aquaculture Research

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Nocardia seriolae, an opportunistic and facultative intracellular Gram‐positive bacterium, is mainly responsible for nocardiosis in fish. Nocardiosis is a chronic, granulomatous and systemic disease in which lesions are found in the skin as well as several internal organs such as the kidney, liver and spleen with the distinctive nodular granuloma structure. N. seriolae is capable of infecting a wide variety of fish species which poses a devastating impact on the aquaculture sector around the globe, particularly in Asia. Due to the lack of appearance of nocardiosis symptoms at the earlier stages, multiple epidemics of nocardiosis causing high mortality have been recorded. To this end, numerous epidemiological studies have been carried out to characterize and identify Nocardia spp. through several detection techniques, which have favoured the formulation of a multitude of antibiotics and vaccines. After bacterial resistance to antibiotics, nowadays, vaccination has been considered the more appropriate strategy to treat nocardiosis. For valuable information related to N. seriolae and to combat fish nocardiosis, this review extended the existing knowledge, including pathogenesis, virulence characteristics, rapid detection techniques, host immune response and prevention strategies against N. seriolae infection in fish species in aquaculture.

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“…The red snapper ( Lutjanus erythropterus ) is one of the most common edible marine fishes cultured in southern Taiwan (Organization of the Fisheries Agency, 2019). Analytical examinations of cultural conditions and nutritional factors, as well as disease outbreaks that endanger these aquaculture practices, have been given prominence in recent years due to its enormous commercial value (Liao et al., 2021). Aside from the bacterial and viral infections that cause mass mortality of fish, parasites have been proven to be a major cause of retarded growth and economic losses in aquaculture industry (Wang et al., 2005).…”

Section: Introductionmentioning

confidence: 99%

First report of acanthocephalan parasite, Longicollum pagrosomi Yamaguti, 1935 in cultured red snapper (Lutjanus erythropterus) in Taiwan

Cheng

Rao

Wang

et al. 2022

Journal of Fish Diseases

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For the first time, Longicollum pagrosomi Yamaguti, 1935, an acanthocephalan parasite, has been characterized with morphological, histopathological and molecular detail in farmed red snapper (Lutjanus erythropterus) in Taiwan. The diseased fish showed clinical signs of anorexia, emaciation and were gasping for air at the water's surface. Gross examination revealed extensive necrosis in the intestine and even penetration at the site of parasitic attachment, resulting in a large number of parasites being lodged in the peritoneal cavity, surrounded by black hyperplastic connective tissue. The parasites collected from the intestine were studied using optical microscopy, histopathology, scanning electron microscopy (SEM) and molecular phylogenetic analysis. They were creamy white in colour, and were separated into their proboscis, neck and metasoma (trunk) under optical microscopy. Histopathological examination revealed ovarian balls (floating ovaries) inside the ligament sac and eggs within the egg sorting apparatus. The SEM images revealed that the proboscis had 11–14 longitudinal rows with 9–12 recurrent, backward‐facing, organized hooks. In the 18S and 28S phylogenetic tree, sequences of the specimens were identical to those of other Longicollum pagrosomi, in addition to the morphological features. Thus, we confirmed that the parasites belonged to Longicollum pagrosomi in this study.

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Isolation and characterization of Nocardia seriolae , a causative agent of systematic granuloma in cultured East Asian four finger threadfin, Eleutheronema rhadinum, and red snapper, Lutjanus erythropterus

Cited by 13 publications

References 27 publications

Simple and cost-effective SNP genotyping method for discriminating subpopulations of the fish pathogen, Nocardia seriolae

Simple and cost-effective SNP genotyping method for discriminating subpopulations of the fish pathogen, Nocardia seriolae

Pathogenicity, diagnosis, prevention strategies and immune response of bacterium Nocardia seriolae : A critical review

First report of acanthocephalan parasite, Longicollum pagrosomi Yamaguti, 1935 in cultured red snapper (Lutjanus erythropterus) in Taiwan

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