Isolation and characterization of Pseudomonas aeruginosa from sheep with fleece rot in northern and middle Jordan

Abstract

A total of 162 sheep fleece samples were collected from 2315 sheep clinically examined for evidence of dermatitis. The sheep belonged to 32 flocks raised in northern and middle Jordan. Eighty-three samples showed no obvious abnormalities, whereas the remainder showed exudation (79 samples), fleece discoloration (18) and fleece roughness (40) and abscesses (7). Seventeen Pseudomonas aeruginosa isolates were obtained from these samples. Antibacterial resistance in vitro was common; resistance to tetracycline, amoxycillin, erythromycin and cotrimoxazole was shown by > 90% of the isolates. Resistance to norfloxacin (29.4% of isolates), ciprofloxacin (17.6%) and amikacin (17.6%) was also demonstrated. Fourteen isolates were serum resistant when assessed after 1-3 h incubation in sheep and calf sera, and their count increased by 2-2.9 and 2.5-3.5 respectively.

Purulent nasomaxillary and mandibular osteomyelitis in sheep caused by Pseudomonas aeruginosa

“…Significant isolations of this species from skin, ear, mammary and lung infections have been reported (Watson and others 2003). Water can act as an important medium for the spread of Pseudomonas infections, particularly in cases of mastitis, rhinitis and otitis (Kingsford and Raadsma 1997, Las Heras and others 1999, El‐Sukhon 2002, Watson and others 2003, Leitner and Krifucks 2007). This short communication describes an outbreak of purulent nasomaxillary and mandibular osteomyelitis associated with P aeruginosa .…”

“…Mandibular and maxillary osteomyelitis has been documented in four red kangaroos ( Macropus rufus ) from which a mixed population of bacteria was cultured, but Pseudomonas species predominated in all of the isolates (Brookins and others 2008). On the other hand, Pseudomonas species appear to be important, given their ubiquity, the ease with which they grow and their resistance to many antibiotics (Krieg and Holt 1984, El‐Sukhon 2002). P aeruginosa causes infections in external organs, such as the mammary glands, skin, nasal cavity or ears.…”

“…P aeruginosa causes infections in external organs, such as the mammary glands, skin, nasal cavity or ears. In these cases, contaminated water has been reported to be the origin of the bacteria (Kingsford and Raadsma 1997, Las Heras and others 1999, El‐Sukhon 2002, Watson and others 2003, Leitner and Krifucks 2007). In the present study, drinking water was also the likely source of the bacterial infection because no further cases occurred after the drinking trough was cleaned and the water supply changed.…”