Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth

Abstract

Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. The present work deals with isolation, characterization and identification of indole acetic acid producing bacteria from the rhizospheric soil. Out of ten Indole acetic acid producing isolates, five were selected as efficient producers. Optimization of indole acetic acid production was carried out at different cultural conditions of pH and temperature with varying media components such as carbon and nitrogen source, tryptophan concentration. Partial purification of IAA was done and purity was confirmed with Thin layer chromatography. Subsequently, effect on plant growth was tested by pot assay. In conclusion the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.

Assessment of Rice Associated Bacterial Ability to Enhance Rice Seed Germination and Rice Growth Promotion

“…amples ere ept at C in refrigerator. The epiphytic, endophytic and rhizospheric culturable bacteria were isolated according to Lindow et al (1978), Sturz et al (1997) and Mohite (2013), respectively. For isolation of epiphytic bacteria, rice samples of leaves and stems were cut in to small sizes and separately put in 500 ml flasks with 200 ml of 0.1 M phosphate buffer (pH:7.0) and 1 g/l peptone.…”

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

“…Burkholderia cepacia IBSD-LKBI produced significant level of IAA at the rate of 58.4 lg/ml in culture filtrate (Devi et al 2012). The conversion of tryptophan into IAA is the most important mechanism of soil microbes (Mohite 2013). Tryptophan-dependant IAA synthesis had been also determined in several soil microbes such as Pseudomonas fluorescens, Pseudomonas spp., Azotobacter spp., Azospirillum spp., Phosphobacterium spp.…”

Integrated Effect of Rhizobium and Azotobacter Cultures on the Leguminous Crop Black Gram (Vigna mungo)

“…Therefore, its use is an eco-friendly approach to reduce the utilization of chemical fertilizers, enhance soil fertility and increase crop production by their biological activity in the rhizosphere. Several bacteria like Pseudomonas, Azospirillum, Azotobacter, Klebsiella, Enterobacter, Alcaligenes, Arthrobacter, Burkholderia, Rhizobium, Flavobacteriaum, Bacillus and Serratia, phosphobacteria and VAM fungi have been used as biofertilizers supplement of nitrogen and phosphorus fertilizers for improved crop production [7][8][9][10][11][12][13][14][15][16].…”