Isolation and characterization of insulin in Russian sturgeon (<i>Acipenser guldenstaedti</i>)

Rusakov, Yu. I.; Moriyama, Shunsuke; Бондарева, В. М.; Kolychev, A. P.; Amemiya, Yutaka; Yasuda, Akikazu; Kawauchi, Hiroshi

doi:10.1111/j.1399-3011.1998.tb00637.x

Cited by 15 publications

(2 citation statements)

References 18 publications

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“…Insulin itself consists of an A-chain usually of 21 amino acids and a B-chain usually of 30 amino acid residues connected by two disulfide bonds. In Russian sturgeon, the amino acid sequence of sturgeon insulin (A-chain; 21-amino-acid peptide, H-Gly-Ile-Val-Glu-Gln-Cys-Cys-His-Ser-Pro-Cys-Ser-Leu-Tyr-Asp-Leu-Glu-Asn-Tyr-Cys-Asn-OH and B-chain; 31-amino-acid peptide, H-Ala-Ala-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Asn-Lys-Val-OH) is more similar to the amino acid sequence of mammalian insulins than of other fish insulins (Rusakov et al, 1998). The homeostatic role of insulin in mammals was succinctly summarized by Tashima and Cahill (1968).…”

Section: Discussionmentioning

confidence: 91%

Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus

Lee

Lim

et al. 2012

Asian Australas. J. Anim. Sci

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A series of studies were carried out to investigate the supplemental effects of dietary garlic extracts (GE) on whole body amino acids, whole body and muscle free amino acids, fatty acid composition and blood plasma changes in 6 month old juvenile sterlet sturgeon (Acipenser ruthenus). In the first experiment, fish with an average body weight of 59.6 g were randomly allotted to each of 10 tanks (two groups of five replicates, 20 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the level of 2% of fish body weight per day for 5 wks. Whole body amino acid composition between the GE and control groups were not different (p>0.05). Among free amino acids in muscle, L-glutamic acid, L-alanine, L-valine, L-leucine and L-phenylalanine were significantly (p<0.05) higher in GE than in control. However, total whole body free amino acids were significantly lower in GE than in control (p<0.05). GE group showed higher EPA (C22:6n3) and DHA (C22:5n3) in their whole body than the other group (p<0.05). In the second experiment, the effects of dietary garlic extracts on blood plasma changes were investigated using 6 month old juvenile sterlet sturgeon averaging 56.5 g. Fish were randomly allotted to each of 2 tanks (300 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the rate of 2% of body weight per day for 23 d. At the end of the feeding trial, blood was taken from the tail vein (n = 5, per group) at 1, 12, and 24 h after feeding, respectively. Blood plasma glucose, insulin and the other serological characteristics were also measured to assess postprandial status of the fish. Plasma glucose concentrations (mg/dl) between two groups (GE vs control) were significantly (p< 0.05) different at 1 (50.8 vs 62.4) and 24 h (57.6 vs 73.6) after feeding, respectively, while no significant difference (p>0.05) were noticed at 12 h (74.6 vs 73.0). Plasma insulin concentrations (μIU/ml) between the two groups were significantly (p<0.05) different at 1 (10.56 vs 5.06) and 24 h (32.56 vs 2.96) after feeding. The present results suggested that dietary garlic extracts could increase dietary glucose utilization through the insulin secretion, which result in improved fish body quality and feed utilization by juvenile sterlet sturgeon.

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Section: Discussionmentioning

confidence: 91%

Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus

Lee

Lim

et al. 2012

Asian Australas. J. Anim. Sci

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“…The life cycle of this species was closed in 1969 and most studies have focused on its systematics and general biology in the last half‐century, but the rearing trial of sturgeons for meat production on farm is in infancy (Rusakov et al . ; Memiş et al . ).…”

Section: Introductionmentioning

confidence: 95%

Effects of dietary lipid sources on growth performance, lipid metabolism and antioxidant status of juvenile Russian sturgeonAcipenser gueldenstaedtii

Zhu

Wei

et al. 2016

Aquacult Nutr

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Six isonitrogenous and isoenergetic diets were used to test the influence of lipid source on growth performance, antioxidant status and lipid metabolism of juvenile Russian sturgeon, Acipenser gueldenstaedtii. Each diet was supplemented with 90 g kg À1 of lipid from each of six sources including fish oil (FO), beef tallow (BT), sunflower oil (SO), linseed oil (LO) and equal combinations of FO + SO + BT (FSB) or LO + SO + BT (LSB). After 56 days, fish fed LSB demonstrated highest weight gain, specific growth rate and lowest hepatosomatic index among all groups. The n-6 polyunsaturated fatty acids (PUFAs) in the whole fish were highest in the SO group, and n-3 PUFAs were highest in fish fed LO. The fish fed FO contained highest n-3 highly unsaturated fatty acids. Triglyceride in the serum of fish fed LSB was lowest, but was not significantly different from that in the SO group. Triglyceride in the serum of fish fed FO and BT was highest among all groups. Lipase, malate dehydrogenase and lipoprotein lipase activities were highest in fish fed LSB. Serum malondialdehyde in fish fed LSB was significantly lower than in fish fed FO or SO, but no significant differences were found among fish fed LSB, BT, LO or FSB. Fish fed LSB showed higher catalase activity and total antioxidant capacity than fish fed FO or FSB. This study indicates that linseed, sunflower oil and BT mixed oil are a suitable lipid source and can benefit growth performance and antioxidation in juvenile sturgeon.

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Gastroenteropancreatic Hormones (Insulin, Glucagon, Somatostatin, and Multiple Forms of PYY) from the Pallid Sturgeon, Scaphirhynchus albus (Acipenseriformes)

Kim

Gadsbøll²,

Whittaker³

et al. 2000

General and Comparative Endocrinology

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Isolation and characterization of insulin in Russian sturgeon (Acipenser guldenstaedti)

Cited by 15 publications

References 18 publications

Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus

Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus

Effects of dietary lipid sources on growth performance, lipid metabolism and antioxidant status of juvenile Russian sturgeonAcipenser gueldenstaedtii

Gastroenteropancreatic Hormones (Insulin, Glucagon, Somatostatin, and Multiple Forms of PYY) from the Pallid Sturgeon, Scaphirhynchus albus (Acipenseriformes)

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