Isolation and characterization of lignin-degrading bacterium Bacillus aryabhattai from pulp and paper mill wastewater and evaluation of its lignin-degrading potential

Abstract

This study reports the degradation and decolourization capability of a manganese peroxidase enzyme producing bacterium isolated from pulp and paper mill wastewater. The isolate was identified as Bacillus aryabhattai based on biochemical analysis and 16S rRNA gene sequencing. The strain was designated MG966493. This bacterium was able to reduce 67% and 54% colour and lignin, respectively, from the pulp and paper mill wastewater after 144 h of treatment at 32 °C, pH 7.6 and 120 rpm. Further, FT-IR analysis showed that during the lignin degradation process a number of metabolites were produced comprising different functional groups such as carbonyl (C=C), carboxyl (-COOH), alkene (C=C), amines (-NH 2 ), sulphonic (-SO 3 ) and nitro (-NO 2 ). In addition, the SEM analysis showed that the bacterial cells exposed to pulp and paper mill wastewater have rough surfaces with reduced size as compared to the unexposed cells with smooth surfaces. This study concluded that the isolated bacterium B. aryabhattai has significant potential for the bioremediation of pulp and paper mill wastewater and thus, can be applied for their treatment at an industrial scale.

A novel strain of Planomicrobium isolated from paper mill and its capacity of cellulose degradation

“…Paper mills are an appropriate and adaptable environment for providing bacteria and fungus on development, reproduction, and nutrition (Pokhrel and Viraraghavan 2005;Roest K et al 2005;Zwieten et al 2010;Shi et al 2018;Su et al 2018;Zainith et al 2019). The present work represents the first report of Planomicrobium isolated from a paper machine along the Yangtze River in China, and it was expected to have biodegradation ability.…”

Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

“…This may suggest that the microbes showed laccase activity (Tian et al, 2020). Only three isolates (CH2, CH5 and CH9) were able to decolourise phenol red dye indicating the production of MnP enzyme that oxidises the dye and removes the original red colour of phenol (Zainith et al, 2019).…”

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

“…Also, it can be useful in reducing pollution from pulp and paper wastewater. B. aryabhattai MG966493 reduced 67% and 54% color and lignin, respectively, from the pulp and paper mill wastewater ( Zainith et al 2019 ). At present, many studies have been carried out on the degradation ability of lignocellulose, but most of the research objects are alkaline lignin, sodium lignosulfonate, and other lignin model compounds ( Zhu et al 2017 ).…”

“…In contrast, lignin-degrading bacteria could also be found in black liquor. B. aryabhattai MG966493 was isolated from pulp and paper mill wastewater, and its Mnp activity peak at 24 h showed 6.1 U · l –1 · ml –1 ( Zainith et al 2019 ). In addition, Chen and coworkers isolated Comamonas sp.…”