Isolation and characterization of Listeria monocytogenes in Chinese food obtained from the central area of China

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

“…We discovered that inlA, inlB, inlC, and inlJ internalin genes which stimulates the adhesion and internalization of L. monocytogenes within the host's epithelial cells [51] occurred in 100% of the isolates recovered from both irrigation water and agricultural soil samples. This is in tune with the findings of [3], except for inlB where they observed a lower prevalence of 71.4%. The high prevalence of the internalin virulence genes in this study corroborates with the findings of [52][53][54].…”

“…A 100% prevalence of the actA gene, which stimulates actin polymerization and causes L. monocytogenes to spread in the host cells was observed in this study. This rate is quite higher than that observed in the study of [3] which is 95% and much more higher than that obtained in the studies of [55]. The prevalence of the hylA and plcB genes in the present study was 100%.…”

“…The hylA and plcB mediate the release of the bacterial cells into the cytoplasm of the host and promotes the escape of the bacteria from bound vacuoles and the surface protein actin A respectively [8]. This rate is in tune with the prevalence rate observed in the study of [3]. In this study, plcA and iap which code for phospholipase and invasion-associated protein respectively, occurred in 100% of the isolates.…”

“…Studies on the prevalence of L. monocytogenes in irrigation water and agricultural soil is rare. However, the prevalence of L. monocytogenes obtained in this study (14%) is relatively low when related to the findings of [44,47] in surface water bodies and WWTPs effluents, but also higher than 2.3% in Chinese foods in the studies of [3]. In irrigation water samples, the highest prevalence was in s10 at a prevalence rate of 25%.…”

“…The confirmed isolates were screened for 9 most commonly occurring virulence markers namely inlA, inlB, inlC, inlJ, actA, hlyA, plcA, plcB, and iap in a PCR based technique as described by [3] using the sets of primers shown in S3 Table. The DNA templates of confirmed isolates of L. monocytogenes were extracted using the boiling technique and the PCR reaction mixture was to a final volume of 25 μL as described above. The PCR was carried out using the protocol as follows: 5 minutes, 94˚C; 35(35 seconds, 94˚C; 30 seconds, 52˚C; 1 minute, 72˚C); 10 minutes, 72˚C.…”