Isolation and characterization of <i>escherichia coli</i> in ready-to-eat foods vended in Islamic University, Kushtia

Abstract

Context: Escherichia coli is shed in the feces of warm blooded animals and humans and thus potential for public health. Detection and characterization of E. coli in the ready-to-eat (RTE) foods concerns due to their presence indicates fecal contamination of the food. Objective: To identify, characterize and RFLP pattern analysis of E. coli isolated from RTE foods vended in Islamic University campus, Kushtia. Materials and Methods: Fifty samples from four types of consumed foods in six student halls of residence, some temporary restaurants of Islamic University, Kushtia were assessed for bacterial contamination by standard methods. Identification and characterization of E. coli isolates were performed using IMViC tests. Genomic DNA was used to perform RFLP pattern analysis. Results: Thirty seven out of 50 (74%) examined samples of RTE foods had E. coli contamination. The highest number of E. coli was isolated from vegetable oriented RTE foods (90.90%) and fish, meat and cereals samples were also significantly E. coli positive. RFLP profiling of two E. coli isolates were observed. Conclusion: The results of this study provide evidence that some RTE foods had unsatisfactory levels of contamination with E. coli. Thus street vended RTE food could be important potential vehicles for food-borne diseases. Molecular characterization may be exploited to identify food borne pathogen among different species. Keywords: Ready-to-eat foods; Escherichia coli; RFLP pattern DOI: http://dx.doi.org/10.3329/jbs.v18i0.8783 JBS 2010; 18(0): 99-103

Microbiological safety of ready‐to‐eat foods in low‐ and middle‐income countries: A comprehensive 10‐year (2009 to 2018) review

“…Abdallah and Mustafa () and Bukar, Uba, and Oyeyi () detected Salmonella in street‐vended juice in Sudan (North Africa) and in zobo from Nigeria (West Africa), respectively. In addition, Biswas, Parvez, Shafiquzzaman, Nahar, and Rahman () and Yannick, Rawlings, & Emmanuela () reported the presence of E. coli in RTE meat from Bangladesh (Asia) and Cameroon (Central Africa), respectively, whereas diarrheagenic E. coli strains were recovered from grilled chicken in Burkina Faso (West Africa) (Somda et al., ). Similarly, Pseudomonas was detected in street‐vended juice in Pakistan, (Batool, Tahir, Rauf, & Kalsoom, ), whereas in Bangladesh (Asia) and Ethiopia, Klebsiella was found to contaminate chotpoti and ambasha , respectively (Eromo, Tassew, Daka, & Kibru, ; Hassan et al., ).…”

A study about the young consumers' consumption behaviors of street foods

“…There are many epidemiological studies about relation of street food and diseases, because of lack of knowledge about hygiene, and food safety of street food seller's and the overabundance of pathogenic bacteria in street food samples [12][13][14] . In many countries around the world, especially in Africa, Asia, and Latin America, it has been found that street foods carry harmful pathogens and cause serious foodborne diseases 12,[15][16][17][18][19][20][21][22][23] .…”

“…Increase in global trade, traveling, changes in consumption of modern food, influence of modern life styles, and changes in food consumption have all caused new pathogens to form 27 . In many countries besides the fact that street food is not reliable, it is seen that they also carry diseases originating from food sources 12,16,20,21,35 . Although street foods carry health risks, they are good food alternatives for students and also offer people with low incomes and those working in small businesses a chance to meet their nutritional needs 36,37 .…”

Street food consumption in terms of the food safety and health

“…Biswas et al (2010) found that from out of 50 food samples, 37 of them (74%) had E.coli contamination and that foods containing vegetables had E.coli at a high percentage (91%), and that these organisms also existed in samples of fish, meat, and cereals and that food sellers could be carrying diseases originating from food sources. In China, Liu et al (2015) found that disease outbreaks originating from Vibrio parahaemolyticus had a high occurance rate.…”

“…The wide consumption of street food around the world increase the importance of safety and health issues (Canet and N'Diaye, 1996). Street foods are not reliable, they also carry diseases originating from food sources in many countries (Omemu and Aderoju, 2008;Biswas et al, 2010;Nunes et al, 2010;Mamun et al, 2013).…”