Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant Staphylococcus aureus clinical isolates

Samir, Safia; El-Far, Amira; Nasr, Sami Mohamed; Mahdy, Rania; Samir, F.; Okasha, Hend

doi:10.1016/j.sjbs.2022.03.019

Cited by 18 publications

(8 citation statements)

References 40 publications

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“…Without viable alternatives to current antibiotics, estimates indicate that by 2050, AMR could lead to cost of hundred trillion USD globally and result 10 million annually deaths. 22 The message is clear: we must seek alternative treatments or face a future where once curable illnesses become preventable fatalities. The increasing levels of antibiotics in the environment, driven by medical and agricultural demand, have severe consequences on microbial ecosystems, rapidly spreading antibiotic resistance worldwide and posing a genuine threat to healthcare.…”

Section: Discussionmentioning

confidence: 99%

“…Without viable alternatives to existing antibiotics, we are facing a future where the consequences of AMR could be devastating. 22 We must actively seek out alternative treatments or be prepared to witness once curable illnesses leading to preventable fatalities. Excessive antimicrobial utilization in both medical and agricultural settings has contributed to the increasing levels of antibiotics in the environment, causing a range of consequences on microbial ecosystems.…”

Section: Opinion and Future Perspectivesmentioning

confidence: 99%

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Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

Abebe,

Birhanu

2023

IDR

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Antimicrobial resistance (AMR) represents a major threat to global health. Infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) is one of the well-recognized global public health problem globally. In some regions, as many as 90% of S. aureus infections are reported to be MRSA, which cannot be treated with standard antibiotics. WHO reports indicated that MRSA is circulating in every province worldwide, significantly increasing the risk of death by 64% compared to drug-sensitive forms of the infection which is attributed to its antibiotic resistance. The emergence and spread of antibiotic-resistant MRSA strains have contributed to its increased prevalence in both healthcare and community settings. The resistance of S. aureus to methicillin is due to expression of penicillin-binding protein 2a (PBP2a), which renders it impervious to the action of β-lactam antibiotics including methicillin. The other is through the production of beta-lactamases. Although the treatment options for MRSA are limited, there are promising alternatives to antibiotics to combat the infections. Innovative therapeutic strategies with wide range of activity and modes of action are yet to be explored. The review highlights the global challenges posed by MRSA, elucidates the mechanisms underlying its resistance development, and explores mitigation strategies. Furthermore, it focuses on alternative therapies such as bacteriophages, immunotherapy, nanobiotics, and antimicrobial peptides, emphasizing their synergistic effects and efficacy against MRSA. By examining these alternative approaches, this review provides insights into the potential strategies for tackling MRSA infections and combatting the escalating threat of AMR. Ultimately, a multifaceted approach encompassing both conventional and novel interventions is imperative to mitigate the impact of MRSA and ensure a sustainable future for global healthcare.

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Section: Discussionmentioning

confidence: 99%

Section: Opinion and Future Perspectivesmentioning

confidence: 99%

Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

Abebe,

Birhanu

2023

IDR

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“…Various research effects on phage application methods have established phage therapy as a promising alternative treatment. Multiple studies have shown that isolated phages from different environmental sources exhibit high specificity towards specific bacterial strains, particularly against multidrug S. aureus [114][115][116][117][118]. Phage cocktails, composed of lytic bacteriophages individually targeting specific pathogenic strains, have shown significant results, influencing their administration [119][120][121][122][123].…”

Section: Exploring the Potential Of Phage Therapy In Modern Medicinementioning

confidence: 99%

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Gopikrishnan,

Haryini,

2024

J Basic Microbiol

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In recent years, antibiotic therapy has encountered significant challenges due to the rapid emergence of multidrug resistance among bacteria responsible for life‐threatening illnesses, creating uncertainty about the future management of infectious diseases. The escalation of antimicrobial resistance in the post‐COVID era compared to the pre‐COVID era has raised global concern. The prevalence of nosocomial‐related infections, especially outbreaks of drug‐resistant strains of Staphylococcus aureus, have been reported worldwide, with India being a notable hotspot for such occurrences. Various virulence factors and mutations characterize nosocomial infections involving S. aureus. The lack of proper alternative treatments leading to increased drug resistance emphasizes the need to investigate and examine recent research to combat future pandemics. In the current genomics era, the application of advanced technologies such as next‐generation sequencing (NGS), machine learning (ML), and quantum computing (QC) for genomic analysis and resistance prediction has significantly increased the pace of diagnosing drug‐resistant pathogens and insights into genetic intricacies. Despite prompt diagnosis, the elimination of drug‐resistant infections remains unattainable in the absence of effective alternative therapies. Researchers are exploring various alternative therapeutic approaches, including phage therapy, antimicrobial peptides, photodynamic therapy, vaccines, host‐directed therapies, and more. The proposed review mainly focuses on the resistance journey of S. aureus over the past decade, detailing its resistance mechanisms, prevalence in the subcontinent, innovations in rapid diagnosis of the drug‐resistant strains, including the applicants of NGS and ML application along with QC, it helps to design alternative novel therapeutics approaches against S. aureus infection.

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“…Staphylococcus aureus is a Gram-positive pathogen that causes a variety of diseases, including skin infections, respiratory infections, and food poisoning in animals and humans (Su et al, 2020). Moreover, because of antibiotic overuse, S. aureus has gradually evolved as a pathogen, such as methicillin-resistance S. aureus (MRSA), with the ability to cause life-threatening infections spreading rapidly worldwide (Samir et al, 2022). A recent conducted survey of food products, covering most of the provincial capitals of China, revealed that high prevalence of S. aureus was found in meat products as well as pasteurized milk (Zhang et al, 2022a)…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a broad-spectrum phage SapYZU11 and its potential application for biological control of Staphylococcus aureus

Wen

Yuan

et al. 2023

Qual. Assur. Saf. Crops Foods

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Staphylococcus aureus, a prominent pathogen, is frequently encountered in clinical and food-processing settings. Given its ability to develop antimicrobial resistance, effective control strategies are required to ensure microbial safety. In this study, four lytic S. aureus phages (SapYZU10, SapYZU11, SapYZU12, and SapYZU13) were isolated from sewage samples in Yangzhou, China. Their biological characteristics and bactericidal effect against S. aureus isolates in vitro and in milk and fresh pork were evaluated. Their activities remained relatively stable under stressful conditions (-80–70°C, pH 3.0–12.0). Notably, SapYZU11 (100%, 53/53) effectively lysed all 53 S. aureus strains, followed by SapYZU12 (90.57%, 48/53), SapYZU13 (79.25%, 42/53), and SapYZU10 (71.70%, 38/53). Among the phages with short latent periods (10–20 min), SapYZU11 had a larger burst size (152.00 plaque forming units [PFU]/mL) and no genes related to antibiotic resistance and virulence. Furthermore, SapYZU11 effectively eradicated S. aureus and its cocktail (YZUsa1, YZUsa4, YZUsa12, YZUsa14, and methicillin-resistance S. aureus (MRSA) JCSC 4744) in Luria–Bertani broth and both food items. Particularly in milk, SapYZU11 with a multiplicity of infection (MOI) of 100 inhibited MRSA JCSC 4744 strain and S. aureus cocktail with maximum reduction levels of 5.03 log (Lg) colony-forming unit (CFU)/mL and 2.80 Lg CFU/mL, respectively. Conversely, contaminated pork treated with three MOIs of SapYZU11 at 25°C and 4°C resulted in reductions of 0.29–1.29 Lg CFU/mL and 0.11–0.32 Lg CFU/mL, respectively. Therefore, SapYZU11 proved as a promising biocontrol agent against S. aureus in different food production settings.

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Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant Staphylococcus aureus clinical isolates

Cited by 18 publications

References 40 publications

Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Isolation and characterization of a broad-spectrum phage SapYZU11 and its potential application for biological control of Staphylococcus aureus

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