Isolation and characterization of macromolecular protein R-Phycoerythrin from Portieria hornemannii

Senthilkumar, N; Suresh, Veeraperumal; Thangam, Ramar; Kurinjimalar, C.; Kavitha, G.; Murugan, Pitchai; Kannan, Soundarapandian; Rengasamy, Ramasamy

doi:10.1016/j.ijbiomac.2012.12.039

Cited by 55 publications

(29 citation statements)

References 25 publications

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“…The supernatant was subjected to 35% followed by 55% saturated ammonium sulfate and each saturation stage, the precipitate was obtained by centrifugation at 12,500 × g for 20 min at 4°C. The precipitate was dialyzed against 0.005 M phosphate buffer at pH 7.2 and the levels of R‐PE, PC, APC, and total protein were estimated as described by Senthil kumar et al, 2013 (Senthilkumar, Suresh, et al, ). The dialyzed solution was lyophilized, stored at −20°C, and named as R‐PE‐rich protein extract (RPERPE).…”

Section: Methodsmentioning

confidence: 99%

“…Several therapeutic products have been isolated from seaweeds of subtropical and tropical populations and few of them have been screened for their anticancer potential (Manilal, et al, ; Xu, Huang, & Cheong, ). Though there are several reports on isolation of bioactive compounds with anticancer potential from marine algae (Gutiérrez‐Rodríguez, Juárez‐Portilla, Olivares‐Bañuelos, & Zepeda, ), algal phycobiliproteins gain huge importance due to their immuno‐enhancing, anti‐inflammatory, anticarcinogenic, antioxidant, and nutritional properties and these properties could provide vital breakthrough in cancer research (Belay, ; Dammeyer & Frankenberg‐Dinkel, ; Senthilkumar, Suresh, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Senthilkumar¹,

Thangam

Murugan

et al. 2018

J Food Biochem

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The present investigation evaluates the hepato‐protective potential of R‐phycoerythrin (R‐PE)‐rich protein extract obtained from Portieria hornemannii (Lyngbye) Silva against H2O2‐induced hepatotoxicity using HepG2 cells (in vitro) and male Wistar albino Hepatocellular carcinoma (HCC) rats (in vivo). H2O2 treatment led to dose‐dependent decreases in cell viability of HepG2, which was ameliorated by the treatment with R‐PE‐rich extract. In vivo studies showed that all the vital parameters including enzymatic and nonenzymatic antioxidants as well as liver marker enzymes were affected in the experimentally induced HCC male Wistar rats upon exposure to the N‐diethylnitrosamine (DEN), a carcinogen. However, there was moderate to better restoration of such parameters in the carcinoma rats treated with R‐PE‐rich protein extract. Practical applications Therapeutic alternatives originating from food or food supplements are gaining popularity as “nutritional therapy” and they are well studied for their chemo‐preventive effects. Investigations of several food‐derived bioactive compounds revealed their ability to antagonize dysregulated targets in cellular signaling pathways to exhibit antineoplastic activities. Isolation of bioactive molecules present in marine food products and determination of their broad range pharmaceutical activity by means of deducing specific molecular targets as well as establishing minimal toxicity to normal tissues could aid in treatment of cancer. Phycobiliproteins are an important group of pigment molecules extracted from the red and blue green algae. A study was envisaged to evaluate the anticancer potential of RPERPE (R‐PE‐rich protein extract) obtained from P. hornemannii (Lyngbye) Silva against HCC using male Wistar albino rats as models. This study offers the scope of using the R‐PE‐rich protein extract of P.hornemannii for developing therapeutic anticancer formulation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Senthilkumar¹,

Thangam

Murugan

et al. 2018

J Food Biochem

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“…R-phycoerythrin (RPE) is commonly used as a fluorescent label [ 39 ]. Phycobiliproteins are known for their immuno-enhancing, anti-inflammatory, anti-carcinogenic, and antioxidant nutritive effects and anticancer properties [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Choi

Gurunathan

Kim

2018

IJMS

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The use of graphene to target and eliminate cancer stem cells (CSCs) is an alternative approach to conventional chemotherapy. We show the biomolecule-mediated synthesis of reduced graphene oxide–silver nanoparticle nanocomposites (rGO–Ag) using R-phycoerythrin (RPE); the resulting RPE–rGO–Ag was evaluated in human ovarian cancer cells and ovarian cancer stem cells (OvCSCs). The synthesized RPE–rGO–Ag nanocomposite (referred to as rGO–Ag) was characterized using various analytical techniques. rGO–Ag showed significant toxicity towards both ovarian cancer cells and OvCSCs. After 3 weeks of incubating OvCSCs with rGO–Ag, the number of A2780 and ALDH+CD133+ colonies was significantly reduced. rGO–Ag was toxic to OvCSCs and reduced cell viability by mediating the generation of reactive oxygen species, leakage of lactate dehydrogenase, reduced mitochondrial membrane potential, and enhanced expression of apoptotic genes, leading to mitochondrial dysfunction and possibly triggering apoptosis. rGO–Ag showed significant cytotoxic potential towards highly tumorigenic ALDH+CD133+ cells. The combination of rGO–Ag and salinomycin induced 5-fold higher levels of apoptosis than each treatment alone. A combination of rGO–Ag and salinomycin at very low concentrations may be suitable for selectively killing OvCSCs and sensitizing tumor cells. rGO–Ag may be a novel nano-therapeutic molecule for specific targeting of highly tumorigenic ALDH+CD133+ cells and eliminating CSCs. This study highlights the potential for targeted therapy of tumor-initiating cells.

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“…Different physico-chemical and enzymatic pretreatments have been suggested to enhance the yields, such as repeated freeze-thaw cycles [201,206], or grinding in liquid nitrogen of the freeze-dried seaweeds [207] aided in the release of R-phycoerythrin. Their further purification from the crude extract has been usually addressed through ammonium sulfate precipitation [208] or also by sucrose step-gradient ultracentrifugation [209] followed by purification by gel filtration and by ion exchange chromatography [130,210,211,212].…”

Section: Components: Properties and Extractionmentioning

confidence: 99%

Integral Utilization of Red Seaweed for Bioactive Production

2019

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The hydrocolloids carrageenan and agar are the major fraction industrially extracted and commercialized from red seaweeds. However, this type of macroalgae also contains a variety of components with nutritional, functional and biological properties. In the context of sustainability and bioeconomy, where the integral utilization of the natural resources is incentivized, the sequential separation and valorization of seaweed components with biological properties of interest for food, nutraceuticals, cosmeceuticals and pharmaceuticals is proposed. In this work, a review of the available conventional and alternative greener and efficient extraction for obtaining red seaweed bioactives is presented. The potential of emerging technologies for the production of valuable oligomers from carrageenan and agar is also commented, and finally, the sequential extraction of the constituent fractions is discussed.

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Isolation and characterization of macromolecular protein R-Phycoerythrin from Portieria hornemannii

Cited by 55 publications

References 25 publications

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Integral Utilization of Red Seaweed for Bioactive Production

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