Isolation and characterization of marine actinomycetes – bioprospecting for antimicrobial, antioxidant and anticancer properties

Abstract

The novel therapeutic agents are frequently produced from marine actinomycetes and are still being explored due to their vast diversity. In this research, we isolated five marine actinomycetes (BN01, BN12, BN13, BN14 and GB15) from the soil sediments of the coastal region of Chennai, Tamil Nadu, India. The morphological and physicochemical properties of the isolates were characterized. The 16S rRNA sequencing analysis showed that the isolates were belonging to two genera namely Streptomyces and Saccharopolyspora. The extracellular crude secondary metabolites were extracted from the culture filtrate of all the marine actinomycetes isolated and tested for the antimicrobial, antioxidant and anticancer potential adopting standard methods. The results revealed that the extracts of the isolates BN01, BN14, and GB15 exhibited antimicrobial activity against M. luteus, E. coli, and S. aureus, with the maximum zone of inhibition of 19 mm, 21 mm, and 27 mm respectively, at the concentration of 10 mg/mL. The extract of the isolate BN12 showed excellent radical scavenging activity of 97.75 % at the concentration of 60 µg/mL and has the half-maximal inhibitory concentration (IC50) of 8.66 µg/mL. The anticancer activity results showed that the extract of the isolate BN12 had better inhibitory activity against the MCF 7 cell line with the IC50 value of 261.01 µg/µL.

Biosynthesis of Silver Nanoparticles From Marine Actinobacterium Micromonospora sp. and Their Bioactive Potential

“…Detailed research has been conducted on the isolation, identification, and taxonomy of actinobacteria from various marine environments. Actinobacteria have attracted for their potential to produce novel compounds for the treatment of various diseases [ 21 ].…”

Actinomycetes: Isolation, Cultivation and its Active Biomolecules

“…32 Soil sediments from the Bay of Bengal shore near Chennai, Tamil Nadu, India, were chosen and dried for a week in sterile conditions. 60 Soil samples from the saltpan of Ennore were collected in sterile polythene bags and preserved at 4°C for further study. 61 South Andaman Island of India, noted for mangrove vegetation offered four marine sediment samples which were taken from the Bay of Bengal coast and the Andaman Sea coast.…”

“…Further, fluconazole (50 µg/mL) was dispensed in media to inhibit the fungal growth. 60 The importance of salt concentration in isolating marine actinomycetes was studied, the absence of NaCl in the culture medium yielded little or no growth, on the other hand isolates were able to grow in 3% NaCl, some isolates were able to grow in 10% NaCl. Further, the study concluded that none of the organisms grew on the media containing salt concentration upto 14%.…”