Isolation and characterization of marine bacteria capable of utilizing phthalate

Iwaki, Hiroaki; Nishimura, Ayaka; Hasegawa, Yoshie

doi:10.1007/s11274-011-0925-x

Cited by 42 publications

(23 citation statements)

References 24 publications

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“…HC degraders were not only cultivated after enrichment cultures on HC, but were also directly detected in the sediment and seawater samples, by sequencing of random DGGE bands. The sequencing of DGGE bands confirms that both sediment and seawater contain known HC degraders as Alcanivorax (alkanes) and Alteromonas (aromatics, Gutierrez et al, 2013) or putative, such as Thalassobius (Iwaki et al, 2012) and Thermoanaerobacterium, often dominant in methanogenic alkanes degrading enrichment culture microcosms (Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 58%

Intrinsic bioremediation potential of a chronically polluted marine coastal area

Catania

Santisi

Signa

et al. 2015

Marine Pollution Bulletin

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Section: Discussionmentioning

confidence: 58%

Intrinsic bioremediation potential of a chronically polluted marine coastal area

Catania

Santisi

Signa

et al. 2015

Marine Pollution Bulletin

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“…Thalassococcus has been shown to be capable of utilizing phthalate (Iwaki et al, 2012), but its ability to metabolize peptides, as suggested here, has not yet been explored. Previous studies have shown that Rhodobacterales are often one of the dominant groups in coastal seawaters, accounting for as high as 75% of the Alphaproteobacteria (Dong et al, 2014).…”

Section: Discussionmentioning

confidence: 95%

Different Bacterial Communities Involved in Peptide Decomposition between Normoxic and Hypoxic Coastal Waters

2017

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Proteins and peptides are key components of the labile dissolved organic matter pool in marine environments. Knowing which types of bacteria metabolize peptides can inform the factors that govern peptide decomposition and further carbon and nitrogen remineralization in marine environments. A 13C-labeled tetrapeptide, alanine-valine-phenylalanine-alanine (AVFA), was added to both surface (normoxic) and bottom (hypoxic) seawater from a coastal station in the northern Gulf of Mexico for a 2-day incubation experiment, and bacteria that incorporated the peptide were identified using DNA stable isotope probing (SIP). The decomposition rate of AVFA in the bottom hypoxic seawater (0.018–0.035 μM h-1) was twice as fast as that in the surface normoxic seawater (0.011–0.017 μM h-1). SIP experiments indicated that incorporation of 13C was highest among the Flavobacteria, Sphingobacteria, Alphaproteobacteria, Acidimicrobiia, Verrucomicrobiae, Cyanobacteria, and Actinobacteria in surface waters. In contrast, highest 13C-enrichment was mainly observed in several Alphaproteobacteria (Thalassococcus, Rhodobacteraceae, Ruegeria) and Gammaproteobacteria genera (Colwellia, Balneatrix, Thalassomonas) in the bottom water. These data suggest that a more diverse group of both oligotrophic and copiotrophic bacteria may be involved in metabolizing labile organic matter such as peptides in normoxic coastal waters, and several copiotrophic genera belonging to Alphaproteobacteria and Gammaproteobacteria and known to be widely distributed may contribute to faster peptide decomposition in the hypoxic waters.

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“…Marine bacteria were collected from each seawater sample by filtration and resuspended in 30 mL of commercial artificial seawater medium Daigo's IMK-SP (Nihon Pharmaceutical, Osaka) [7]. Enrichment and purification of the strains were performed according to a previously described procedure with some modifications [7]; Daigo's IMK-SP medium was supplemented with 5 mM phenol.…”

Section: Isolation Of Phenol-degrading Bacteria From Seawatermentioning

confidence: 99%

Isolation and Genetic Characterization of Phenol-Utilizing Marine Bacteria and Their Phenol Degradation Pathway

Iwaki

Takada²,

Hasegawa³

2015

IJGG

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Phenolic compounds are widely distributed toxic pollutants in seawater, and their effective degradation is very important for bioremediation programs. In this study, nine phenol-degrading bacteria were isolated from seawater samples, which were collected from the coastal areas of Japan. Besides the enrichment substrate phenol, all isolates could utilize at least one isomer of cresol as the sole source of carbon. A 16S rRNA gene sequence analysis indicated that all strains were affiliated with the class Gammaproteobacteria, four strains were closely related to Spongiibacter, four were closely related to Marinobacter, and one was closely related to Photobacterium. During growth on phenol, all isolates produced a yellow product, and a whole-cell study indicated that it was an extradiol meta-ring cleavage product of catechol, 2-hydroxymuconate semialdehyde. Phylogenetic analysis revealed that the partial gene encoding the largest subunit of the multicomponent phenol hydroxylase of the isolates was similar to that of terrestrial bacteria, thereby suggesting that phenol is converted into catechol by marine bacteria. We also suggest that horizontal transfer of the gene may occur not only among marine bacteria but also between the genera Marinobacter and Pseudomonas.

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Isolation and characterization of marine bacteria capable of utilizing phthalate

Cited by 42 publications

References 24 publications

Intrinsic bioremediation potential of a chronically polluted marine coastal area

Intrinsic bioremediation potential of a chronically polluted marine coastal area

Different Bacterial Communities Involved in Peptide Decomposition between Normoxic and Hypoxic Coastal Waters

Isolation and Genetic Characterization of Phenol-Utilizing Marine Bacteria and Their Phenol Degradation Pathway

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