2004
DOI: 10.1080/14653240410005366-1
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Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta

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Cited by 270 publications
(143 citation statements)
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“…Placental sections of about 10 g (2.5 cm cubes) were extensively minced and then centrifuged at 550 g for 5 min. The pellet was then haemolysed in red blood cell lysis buffer (155 mM NH 4 Cl, 10 mM KHCO 3, 0.1 mM EDTA, pH 8.0), centrifuged at 550 g for 5 min at room temperature. The pellet was incubated at 37 C in 0.05% Trypsin-EDTA solution (Invitrogen/Gibco, California, USA) for 10 minutes, resuspended twice in 50% a-MEM medium/ 50% FBS, subjected to centrifugation at 550 g for 10 min at room temperature and then plated in a-MEM medium supplemented with 20% FCS, 0.02% L-ascorbate-2-phosphate, 1% L-Glutamine, 0.5% penicillin/streptomycin, 0.5% Fungizone and 1% Tylosin.…”
Section: Isolation Of Stem Cells From Term Placental Tissuementioning
confidence: 99%
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“…Placental sections of about 10 g (2.5 cm cubes) were extensively minced and then centrifuged at 550 g for 5 min. The pellet was then haemolysed in red blood cell lysis buffer (155 mM NH 4 Cl, 10 mM KHCO 3, 0.1 mM EDTA, pH 8.0), centrifuged at 550 g for 5 min at room temperature. The pellet was incubated at 37 C in 0.05% Trypsin-EDTA solution (Invitrogen/Gibco, California, USA) for 10 minutes, resuspended twice in 50% a-MEM medium/ 50% FBS, subjected to centrifugation at 550 g for 10 min at room temperature and then plated in a-MEM medium supplemented with 20% FCS, 0.02% L-ascorbate-2-phosphate, 1% L-Glutamine, 0.5% penicillin/streptomycin, 0.5% Fungizone and 1% Tylosin.…”
Section: Isolation Of Stem Cells From Term Placental Tissuementioning
confidence: 99%
“…PMSCs selectively attach to the plastic cultureware, proliferate rapidly and are usually prepared without additional enrichment strategies [2]. PMSCs can be differentiated in vitro under specific stimulatory environments into derivatives of the mesenchymal cell lineage such as osteocytes, adipocytes, myocytes and chondrocytes [1,[3][4][5][6][7]. In addition, there is evidence of differentiation in vitro into cell types characteristic of other lineages such as hepatocyte-like cells and neural-like cells [3,4,[7][8][9][10][11], but in vivo evidence for such differentiation is very limited.…”
Section: Introductionmentioning
confidence: 99%
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“…Cells may also be isolated during an ongoing pregnancy using relatively minimally invasive techniques such as chorionic villus sampling (CVS). It was shown that PMSCs are able to differentiate into adipocytes, osteoblasts, myofibroblasts, hepatic cells and neural-like cells under appropriate conditions [67,70]. The potential of PMSCs to differentiate into different cell types, pretested in experiments in vitro, is then confirmed in many experiments in vivo.…”
Section: Placenta/chorionic Villous Mesenchymal Stem/stromal Cellsmentioning
confidence: 78%
“…The large number of sources of MSC-like cells in the adult body is one of the reasons why these cells are so attractive. MSC-like cells have been identified in the dental pulp, 8 skin, 9 umbilical cord blood, 10 placenta, 11 liver and lungs. 5 Under certain in vitro conditions MSCs are capable of differentiating into osteoblasts, adipose cells, cartilage cells, skeletal muscle cells and even into neuron-like cells.…”
Section: Introductionmentioning
confidence: 99%