Isolation and characterization of mesenchymal stromal cells from human anterior cruciate ligament

Huang, TF; Yt, Chen; Yang, Tao; Chen, LL; Sh, Chiou; Th, Tsai; Cc, Tsai; Mh, Chen; Hl, Ma; Hung, Sheng-Che

doi:10.1080/14653240802474323

Cited by 42 publications

(36 citation statements)

References 24 publications

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“…The majority of studies have reported a fibroblastic cell morphology of LDSCs [24, 25]; however, there seems to be variation between ligament types. LDSCs isolated from ACL and posterior cruciate ligament (PCL) demonstrated a fibroblastic morphology [15, 21] and those from MCL a polygonal morphology [18, 20].…”

Section: Characteristics Of Ldscsmentioning

confidence: 99%

Ligament-Derived Stem Cells: Identification, Characterisation, and Therapeutic Application

Lee

Clegg

Comerford

et al. 2017

Stem Cells International

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Ligament is prone to injury and degeneration and has poor healing potential and, with currently ineffective treatment strategies, stem cell therapies may provide an exciting new treatment option. Ligament-derived stem cell (LDSC) populations have been isolated from a number of different ligament types with the majority of studies focussing on periodontal ligament. To date, only a few studies have investigated LDSC populations in other types of ligament, for example, intra-articular ligaments; however, this now appears to be a developing field. This literature review aims to summarise the current information on nondental LDSCs including in vitro characteristics of LDSCs and their therapeutic potential. The stem cell niche has been shown to be vital for stem cell survival and function in a number of different physiological systems; therefore, the LDSC niche may have an impact on LDSC phenotype. The role of the LDSC niche on LDSC viability and function will be discussed as well as the therapeutic potential of LDSC niche modulation.

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Section: Characteristics Of Ldscsmentioning

confidence: 99%

Ligament-Derived Stem Cells: Identification, Characterisation, and Therapeutic Application

Lee

Clegg

Comerford

et al. 2017

Stem Cells International

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“…In one particular study MSC's were isolated from a human ACL and the results demonstrated that both ACL-derived MSC's and bone marrow MSC's expressed marker genes for ligament fibroblasts, but mRNA expression levels for collagen I and III were higher in the ACL-derived MSC's. It was concluded from this study that ACL-derived MSC's have an increased potential to form ligament fibroblasts in comparison to bone marrow MSC's (Huang, Chen et al 2008). Co-culturing MSC's with ligament fibroblast has been shown to successfully induce MSC differentiation into fibroblasts, where this conclusion was based upon the mRNA expression of key ligament genes (collagen I collagen III, and tenascin-c) and synthesis of these key ligament proteins ).…”

Section: Suitable Cell Types For Ligament Tissue Engineeringmentioning

confidence: 99%

“…Due to the absence of specific markers, ligaments can only be distinguished from other ligaments and other tissue types (eg tendon) by structural, molecular and mechanical properties. Although there is no single specific marker in the ligament, tenscin-c has been considered to be a marker due to its characteristically high amounts in the ligament (Doroski, Brink et al 2007), where the presence of collagen types I and III, tenomodulin, biglycan, decorin, elastin and fibronectin are also characteristic of ligament tissue (Vunjak-Novakovic, Altman et al 2004;Chen, Huang et al 2008 …”

Section: Characteristic Components Of Ligament Tissuementioning

confidence: 99%

Tissue Engineering for Tissue and Organ Regeneration

Eberli¹

2011

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“…MSCs have been isolated from a variety of different tissues such as bone marrow, adipose tissue, trabecular bone, and tendon8). Research showed that cells isolated from torn ACLs of young donors possess a surface antigen expression profile highly similar to that of bone marrow-derived mesenchymal stem cells (BMSCs) and they were able to undergo multilineage differentiation, which qualifies them as MSC types9).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Isolated from the Anterior Cruciate Ligament: Characterization and Comparison of Cells from Young and Old Donors

Prager¹,

Kunz²,

Ebert³

et al. 2018

Knee Surg Relat Res

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PurposeMesenchymal stem cells (MSCs) isolated from the anterior cruciate ligament (ACL) share multiple characteristics of bone marrow-derived mesenchymal stem cells (BMSCs), allowing their use for regenerative therapies. Injuries to the ACL can affect people of all ages. This study assesses whether the regenerative potential of ACL-derived MSCs (ACL-MSCs) from old donors is as high as the potential of ACL-MSCs from young donors.Materials and MethodsACL-MSCs were isolated from ACL tissues obtained from young and old donors at the time of ACL reconstruction or arthroplasty. Proliferative capacity, multilineage differentiation potential (chondrogenic, osteogenic, and adipogenic lineages), and transcriptome-wide gene expression were assessed and compared between young and old donors. BMSCs of middle-aged donors served as an additional comparator.ResultsNo substantial differences between ACL-MSCs from young and old donors were observed in their proliferative capacity and multilineage differentiation potential. The latter did not substantially differ between both ACL-MSC groups and BMSCs. Differential expression of genes related to the cytoskeleton and to protein dephosphorylation amongst other pathways was detected between ACL-MSCs from young and old donors.ConclusionsRegenerative potential of ACL-MSCs from old donors was not substantially lower than that from young donors, suggesting that regenerative therapies of ACL tears are feasible in both age groups. In vivo studies of the effect of age on the efficacy of such therapies are needed.

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Isolation and characterization of mesenchymal stromal cells from human anterior cruciate ligament

Cited by 42 publications

References 24 publications

Ligament-Derived Stem Cells: Identification, Characterisation, and Therapeutic Application

Ligament-Derived Stem Cells: Identification, Characterisation, and Therapeutic Application

Tissue Engineering for Tissue and Organ Regeneration

Mesenchymal Stem Cells Isolated from the Anterior Cruciate Ligament: Characterization and Comparison of Cells from Young and Old Donors

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