Isolation and characterization of mesenchymal stem cells from chicken bone marrow

Abstract

The bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells, which can differentiate in vitro into many cell types. However, the vast majority of experimental materials were obtained from human, mouse, rabbit and other mammals, but rarely in poultry. So, in this study, Thirty- to sixty-day old chicken was chosen as experimental animal, to isolate and characterize BMSCs from them. To investigate the biological characteristics of chicken BMSCs, immunofluorescence and RT-PCR were used to detect the characteristic surface markers of BMSCs. Growth curves were drawn in accordance with cell numbers. To assess the differentiation capacity of the BMSCs, cells were induced to differentiate into osteoblasts, adipocytes, and endothelial cells. The surface markers of BMSCs, CD29, CD44, CD31, CD34, CD71 and CD73, were detected by immunofluorescence and RT-PCR assays. The growth curves of different passages were all typically sigmoidal. Karyotype analysis showed that these in vitro cultured cells were genetically stable. In addition, BMSCs were successfully induced to differentiate into osteoblasts, adipocytes, and endothelial cells. The results suggest that the BMSCs isolated from chicken possess similar biological characteristics with those separated from other species, and their multi-lineage differentiation potentiality herald a probable application for cellular transplant therapy in tissue engineering.

Quantitative variations of CD4⁺CD25⁺ cells in Peking duckwhite leghorn chimeras based on bone marrow mesenchymal stem cells

“…As a cell surface glycoprotein, CD44 functions as a growth-anchoring site for MSCs. CD45 is a well-known marker of leukocyte common antigen expressed in virtually all white blood cells and hematopoietic stem cells [22].…”

“…The hematopoietic stem/progenitor cell marker CD34 [22] has been implicated in the transportation of hematopoietic stem cells and in inflammatory responses and lymphocyte homing. CD71, a transferrin receptor, is necessary for iron transport and regulation of the intracellular iron concentration [24].…”

“…BMMSCs can be differentiated into osteoblasts and adipocytes [22]. In this paper, the differentiation of the P3 cells was induced in vitro.…”

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“…10,12,20,21 MSCs are fibroblast-like multipotent progenitor cells which have the ability for self-renewal and a differentiation capacity. 22 MSCs were isolated from different anatomical regions of chicken embryo 23 and postnatal 1-14days old chicken.…”

“…8,9 Avian multipotent stem cells can maintain self-renewal and multilineage differentiation ability in vitro. 10 The vast majority of avian multipotent stem cells have been obtained from different tissue sources including bone marrow, primary kidney, lung, dermis, and other tissues and the stem cell populations derived from these tissues are valuable (Figure 1). Among different kinds of multipotent stem cells, mesenchymal stem cells are the most representative type, which can be differentiate into muscle, adipocytes, cartilage, and osteoblasts in vitro.…”