Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system

Abstract

Fifteen mesophilic bacteria with high C x cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. A CMCase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 U ml ؊1. Although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, respectively. Study of morphological and physiological characters of five predominant isolates which possess high CMCase and had positive effect on synergetic cellulose degradation in mixed culture system showed that two of them were closely related to Bacillus pasteurii and Bacillus cereus, whereas the rest belong to the genus Halobacillus, Aeromicrobium and Brevibacterium, respectively.

Characterization of Cellulolytic Microorganisms Associated with Naturally Decomposing Waste Material

“…Among the cellulolytic bacteria maximum cellulase activity was in isolate B-30 (3.48 cm) followed by isolate B-28 (2.98 cm). These results are in conformity with the results of Lu et al, (2005) who screened fifteen mesophilic bacteria which showed the ability to develop clearing zones around their colonies ranging from 2.55 to 6.40 cm. Similar results have also been reported by Ariffin et al, (2006), Shankar et al, (2011) and Majidi et al, (2011).…”

Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake gold mine, Lead, South Dakota, USA

“…CDB have been isolated from various environments such as compost systems [22,43], soils [1,21], wastewaters [40], and thermal springs [19]. However, to date there is no report of CDB from the deep terrestrial subsurface environments such as those exempliWed by ultra-deep mines which represents an emerging area for exploring the microbial populations with bewildering arrays of metabolic capabilities [10].…”

Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains

“…Thermophilic cellulose-degrading bacteria have been isolated from various environments such as compost systems (Lu et al, 2005;Mayendea et al, 2006;Ng et al, 2009), soils (Abdel-Fattah et al, 2007;Lee et al, 2008), wastewaters (Tai et al, 2004), and from the deep subsurface of the former Homestake gold mine in Lead, SD, now known as the NSF Deep Underground Science and Engineering Laboratory (DUSEL) (Rastogi et al, 2009a). The deep biosphere of gold mines harbors harsh environments similar to those in industrial processes for microorganisms, both natural and anthropogenic, including extreme temperature, pH, pressure, low-oxygen concentration, and inhibitors including toxic metals.…”