Isolation and characterization of metallothioneins from cadmium-loaded mussel Mytilus edulis

Frankenne, Françis; Noël-Lambot, F.; Distèche, A.

doi:10.1016/0306-4492(80)90122-7

Cited by 21 publications

(20 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Zn content was lower in with those obtained from the digestive gland of Cd-exposed mussels in which, as previously demonstrated, cytosolic Cd is mostly bound to a metallothionein (Frankenne et al 1980, Viarengo 1989. Soluble extracts obtained from scallop and mussel digestive gland were analyzed by HPLC on a gel filtration column.…”

Section: Resultsmentioning

confidence: 53%

“…it is a soluble, low-molecular-weight, heat-stable protein with high metal content. Moreover, the absorption spectra of the Cd-protein obtained by diode array detection during HPLC gel filtration are typical of metallothionein, as they have a maximum at 254 nm and are almost identical to the absorption spectrum of the Cd-thionein obtained from Cd-exposed mussels (George et al 1979, Frankenne et al 1980. In addition, the Cd protein present in the 2 Pectinidae, The data on the subcellular distribution of Cd in the digestive gland seem to demonstrate that in the 2 Pectinidae the compartmentalization of Cd in the cell particulate fraction could represent a second mechanism of Cd ion homeostasis (in Adamussiurn colbecki it accounts for about 70 % of total Cd).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cu, Zn and Cd content in different tissues of the Antarctic scallop Adamussium colbecki: role of metallothionein in heavy metal homeostasis and detoxication

Viarengo¹,

Canesi²,

Mazzucotelli³

et al. 1993

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Cu and Zn concentrations in the gills of the Antarctic scallop Adamussiurn colbeck! (Smith, 1902) were found to be significantly lower than in the gills of mussel Mytilus galloprovincialis (Lam ) and scallop Pecten jacobaeus (L.); however, the Cd concentration was significantly higher. In digestive gland, copper concentrations were not significantly different for all 3 species, but A. Colbecki had a lower Zn concentration and an extremely high Cd concentration (ca 27 pg g-' wet wt). In A. colbeckidigestive gland, about 70 % of the total Cd was associated w t h the particulate fraction, and the rest was mostly bound to a metallothionein-type protein. In P. jacobaeus a major fraction (about 60 %) of the Cd present in the digestive gland was bound to cytosolic metallothioneins. Aging was found not to affect the concentration of Cd bound to metallothioneins in A. colbecki digestive gland, although the Cd concentration is slightly reduced in scallops > 10 yr old compared to younger (3 to 7 yr old) scallops.

show abstract

Section: Resultsmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Cu, Zn and Cd content in different tissues of the Antarctic scallop Adamussium colbecki: role of metallothionein in heavy metal homeostasis and detoxication

Viarengo¹,

Canesi²,

Mazzucotelli³

et al. 1993

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…Recent studies have identified the presence of metal-binding proteins, similar to metallothioneins, in several tissues of M. edulis (e.g. gills, hepatopancreas, kidney and mantle), following exposure to cadmium (NoEl-Lambot 1976, George et al 1979, Frankenne et al 1980, K6hler and Riisgfird 1982, Nolan and Duke 1983a, b, Frazier et al 1985, copper (Harrison et al 1988), mercury (Roesijadi etal. 1982, Roesijadi 1986) and silver (George etal.…”

Section: Introductionmentioning

confidence: 97%

Metallothionein induction inMytilus edulis exposed to cadmium

1991

View full text Add to dashboard Cite

Abstract. The exposure of mussels, Mytilus edulis, collected from Whitsand Bay, southwest England, in August 1988, to sublethal concentrations of cadmium (400/~g 1-i) for 65 d resulted in the induction of metallothionein (MT) synthesis in the soft tissues. In cadmium-exposed mussels, metallothionein concentrations, measured by differential pulse polarography, increased by a factor of three, from 2 to 3 mg g-1 to a maximum of 9 mg g-1 after 30 d. No significant changes could be detected in controls. Cadmium accumulated in the soft tissues of mussels correlated significantly with metallothionein concentrations and can be described by the relationship: MT (mg g-1) =0.045 Cd (#g g-1)+ 3.03 (r=0.803, P<0.001). Gel chromatography of heat-treated cytosolic extracts showed that the accumulated cadmium is bound principally to the newly formed metallothioneins. Copper and zinc were also analysed in the whole soft-tissues and in subcellular fractions of cadmium-exposed mussels. Although copper concentrations were not affected by cadmium-exposure, zinc levels were significantly reduced. The results demonstrate that the induction of metallothioneins in M. edulis is a quantifiable biological response to sublethal levels of cadmium exposure.

show abstract

“…To date, metalloproteins and particularly metallothionein-like proteins have been reported in several marine vertebrates (teleost fish: Olafson and Thompson 1974;Bouquegneau et al 1975;Overnell and Coombs 1979;elasmobranchs: Flos and Hidalgo 1983) and invertebrates including Mollusca, Crustacea and Polychaetes (MytiIus edulis: NoID-Lambot 1976;George et al 1979;Marshall and Talbott 1979;Frankenne et al 1980;Roesijadi and Hall 1981 ;Viarengo et al 1980; Crassostrea virginica: Casterline and Yip 1975;Ridlington and Fowler 1979; Patella vulgata: Howard and Nickles 1977;NoID-Lambot et al 1978;NoID-Lambot et al 1980;Patella Bouquegneau et al 1983; Carcinus maenas: Jennings et al 1979; Cancer pagurus: Overnell and Trewhella 1979; Cancer magister and Scylla serrata: Olafson et al 1979a;Eudistylia vancouveri: Young and Roesijadi 1983;etc). However, to date, only some of those metallothionein-like proteins have been proved to be true metallothioneins.…”

Section: Heavy Metal Storage In Marine Animals Under Various Environmmentioning

confidence: 98%

Heavy Metal Storage in Marine Animals Under Various Environmental Conditions

Bouquegneau

Martoja

Truchet³

1984

Proceedings in Life Sciences

View full text Add to dashboard Cite

High loads of heavy metals can be found in some marine species either due to their peculiar physiology or due to the level of pollution of the medium they are living in. The first case is illustrated by copper loads of species using haemocyanin as oxygencarrier and iron loads of species using haemoglobin. The other case is, for example, that of mollusc species living in the Severn Estuary, highly polluted by heavy metals: they contain more Cd, Cu and Zn than molluscs of the same species living in unpolluted mediums (Noel-Lambot et al . .1978). Metabolic and toxic effects of metal ions have been reviewed in mollusca by Simkiss and Mason (1983). Excesses of essential metals as well as the presence in large amounts of non-essential ones, due to inappropriate intracellular binding, may be toxic for those species when the excess of metal is not stored in the animal under a detoxified form. Heavy metals can be stored an detoxified by marine organisms either by a compartmentation process within membrane-litnited vesicles or by binding to specific proteins.

show abstract

Isolation and characterization of metallothioneins from cadmium-loaded mussel Mytilus edulis

Cited by 21 publications

References 21 publications

Cu, Zn and Cd content in different tissues of the Antarctic scallop Adamussium colbecki: role of metallothionein in heavy metal homeostasis and detoxication

Cu, Zn and Cd content in different tissues of the Antarctic scallop Adamussium colbecki: role of metallothionein in heavy metal homeostasis and detoxication

Metallothionein induction inMytilus edulis exposed to cadmium

Heavy Metal Storage in Marine Animals Under Various Environmental Conditions

Contact Info

Product

Resources

About