Isolation and characterization of methicillin-resistant Staphylococcus aureus from fire stations in two northwest fire districts

Roberts, Marilyn C.; Soge, Olusegun O.; No, David; Beck, Nicola K.; Meschke, John Scott

doi:10.1016/j.ajic.2010.09.008

Cited by 32 publications

(58 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PFGE analyses were done as previously described (7,16). The DNA blocks were prepared, digested separately with SmaI (Fermentas Inc., Glen Burnie, MD) for 6 h at 28°C, and subjected to electrophoresis in a contour-clamped homogeneous electric field (CHEF DR II system; Bio-Rad Laboratories Inc., Hercules, CA) for 21 h at 14°C with switch times of 5 s (initial) and 40 s (final) at 6 V cm Ϫ1 as described previously (7).…”

Section: Methodsmentioning

confidence: 99%

Impact of Strain Typing Methods on Assessment of Relationship between Paired Nares and Wound Isolates of Methicillin-Resistant Staphylococcus aureus

et al. 2013

Self Cite

View full text Add to dashboard Cite

The anterior nares are the site of choice for the Veterans Administration methicillin-resistant Staphylococcus aureus (MRSA) surveillance program; however, a correlation between nares colonization and concomitant wound infections has not been well established. The purpose of this study was 3-fold: to determine the relatedness of MRSA isolates from 40 paired wound and nares specimens by four different strain typing methods, to determine concordance of typing methods, and to establish a baseline of MRSA types at this medical center. Isolates were typed by repetitive PCR (rep-PCR) (DiversiLab System; DL) and SpectraCell Raman analysis (SCRA) (commercially available methods that can be performed within a clinical lab), pulsed-field gel electrophoresis (PFGE), and an antibiotic susceptibility profile (AB). Whole-genome optical mapping (WGM) (OpGen, Inc.) was performed on selected isolates. All methods agreed that 26 pairs were indistinguishable and four pairs were different. Discrepant results were as follows: 4 where only SCRA was discordant, 3 where only AB was discordant, 2 where both DL and AB were discordant, and 1 where both DL and SCRA were discordant. All WGM agreed with PFGE. After discrepancy resolution, 80% of the pairs were indistinguishable and 20% were different. A total of 56% of nares results were nonpredictive if negative nares and positive wound cultures are included. Methods agreed 85 to 93% of the time; however, congruence of isolates to a clade was lower. Baseline analysis of types showed that 15 pairs were unique to single patients (30 strains, 38%; 47% of the matching pairs). Twenty-five strains (30%) represented a single clade identical by PFGE, SCRA, and DL, decreasing specificity. Typing method and institutional type frequency are important in assessing MRSA strain relatedness.

show abstract

Section: Methodsmentioning

confidence: 99%

Impact of Strain Typing Methods on Assessment of Relationship between Paired Nares and Wound Isolates of Methicillin-Resistant Staphylococcus aureus

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, within the last 5 years, MRSA has moved from being primarily a nosocomial pathogen to one that is also found in community areas and public places [7,26]. Community-acquired strains have been documented in areas such as day-care centers [27-29], fire stations [30], and universities [31]. Furthermore, there are many other species of Staphylococcus that are pathogenic, and our understanding of the distribution of these species in public areas is poor [6].…”

Section: Introductionmentioning

confidence: 99%

A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System

Yeh

Simon

Millar

et al. 2011

Osong Public Health and Research Perspectives

View full text Add to dashboard Cite

ObjectivesOur goal was to determine the diversity and abundance of Staphylococcus bacteria on different components of a public transportation system in a mid-sized US city (Portland, Oregon) and to examine the level of drug resistance in these bacteria.MethodsWe collected 70 samples from 2 cm × 4 cm sections from seven different areas on buses and trains in Portland, USA, taking 10 samples from each area. We isolated a subset of 14 suspected Staphylococcus spp. colonies based on phenotype, and constructed a phylogeny from16S rRNA sequences to assist in identification. We used the Kirbye–Bauer disk diffusion method to determine resistance levels to six common antibiotics.ResultsWe found a range of pathogenic Staphylococcus species. The mean bacterial colony counts were 97.1 on bus and train floors, 80.1 in cloth seats, 9.5 on handrails, 8.6 on seats and armrests at bus stops, 3.8 on the underside of seats, 2.2 on windows, and 1.8 on vinyl seats per 8 cm2 sample area. These differences were significant (p < 0.001). Of the 14 isolates sequenced, 11 were staphylococci, and of these, five were resistant to penicillin and ampicillin, while only two displayed intermediate resistance to bacitracin. All 11 isolates were sensitive to trimethoprim-sulfamethoxazole, vancomycin, and tetracycline.ConclusionsWe found six different strains of Staphylococcus, and while there were varying levels of drug resistance, we did not find extensive levels of multidrug-resistant bacteria, and no S. aureus was found. We found floors and cloth seats to be areas on buses and trains that showed particularly high levels of bacteria.

show abstract

“…7 Dark blue colonies were isolated and plated on Brucella agar (Difco Laboratories, Division BD Sparks, MD, USA) supplemented with 5% sterile sheep blood and incubated at 36.58C in 5% CO 2 for 24 h. Colonies that produced b-haemolysis were verified as S. aureus using the Staphaurex w test (Remel, Lenexa, KS, USA) according to the manufacturer's instructions. Verification of MRSA was determined using the Thermo Scientific PBP2' latex agglutination test kit w according to the manufacturer's instructions (Thermo Fisher Scientific, Remel Products, Lenexa, KS, USA).…”

Section: Isolation and Verification Of S Aureus And Mrsamentioning

confidence: 99%

“…7,8 Composite environmental surface samples (n¼56) were collected from the five floors of section 1, where the MRSA was first noted in the primates, as previously described. 7 No environmental samples from sections 2 and 3 were collected.…”

Section: Primate Human and Environmental Samplesmentioning

confidence: 99%

Transmission of MDR MRSA between primates, their environment and personnel at a United States primate centre

Soge

Michael

et al. 2016

J. Antimicrob. Chemother.

Self Cite

View full text Add to dashboard Cite

Objectives: MDR MRSA isolates cultured from primates, their facility and primate personnel from the Washington National Primate Research Center were characterized to determine whether they were epidemiologically related to each other and if they represented common local human-associated MRSA strains.Methods: Human and primate nasal and composite environmental samples were collected, enriched and selected on medium supplemented with oxacillin and polymyxin B. Isolates were biochemically verified as Staphylococcus aureus and screened for the mecA gene. Selected isolates were characterized using SCCmec typing, MLST and WGS.Results: Nasal cultures were performed on 596 primates and 105 (17.6%) were MRSA positive. Two of 79 (2.5%) personnel and two of 56 (3.6%) composite primate environmental facility samples were MRSA positive. Three MRSA isolates from primates, one MRSA from personnel, two environmental MRSA and one primate MSSA were ST188 and were the same strain type by conventional typing methods. ST188 isolates were related to a 2007 ST188 human isolate from Hong Kong. Both MRSA isolates from out-of-state primates had a novel MLST type, ST3268, and an unrelated group. All isolates carried ≥1 other antibiotic resistance gene(s), including tet(38), the only tet gene identified.Conclusions: ST188 is very rare in North America and has almost exclusively been identified in people from Pan-Asia, while ST3268 is a newly reported MRSA type. The data suggest that the primate MDR MRSA was unlikely to come from primate centre employees. Captive primates are likely to be an unappreciated source of MRSA.

show abstract

Isolation and characterization of methicillin-resistant Staphylococcus aureus from fire stations in two northwest fire districts

Cited by 32 publications

References 21 publications

Impact of Strain Typing Methods on Assessment of Relationship between Paired Nares and Wound Isolates of Methicillin-Resistant Staphylococcus aureus

Impact of Strain Typing Methods on Assessment of Relationship between Paired Nares and Wound Isolates of Methicillin-Resistant Staphylococcus aureus

A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System

Transmission of MDR MRSA between primates, their environment and personnel at a United States primate centre

Contact Info

Product

Resources

About