Isolation and characterization of microorganisms involved in the biodeterioration of concrete in sewers

Nica, Dana; Davis, Jeffrey L.; Kirby, Linda; Zuo, Geyan; Roberts, Deborah J.

doi:10.1016/s0964-8305(00)00064-0

Cited by 135 publications

(68 citation statements)

References 19 publications

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“…However, the contribution of A. ferrooxidans to the corrosion process in our experiment must have been small because we found it to be much less abundant than A. thiooxidans. It was recently suggested that fungi also play an important role in the MICC process (7,13,30). We found no microscopic evidence at any time during the monitoring period of a significant presence of fungus-like microorganism after staining samples with DAPI.…”

Section: Succession Of Concrete Corrosion and Microbial Communitycontrasting

confidence: 59%

Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

Okabe

Odagiri

Ito

et al. 2007

Appl Environ Microbiol

305

238

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Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for a long time. A better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of MICC. In this study, the succession of sulfur-oxidizing bacteria (SOB) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes of SOB species were involved in the MICC process, and the predominant SOB species shifted in the following order: Thiothrix sp., Thiobacillus plumbophilus, Thiomonas intermedia, Halothiobacillus neapolitanus, Acidiphilium acidophilum, and Acidithiobacillus thiooxidans. A. thiooxidans, a hyperacidophilic SOB, was the most dominant (accounting for 70% of EUB338-mixed probe-hybridized cells) in the heavily corroded concrete after 1 year. This succession of SOB species could be dependent on the pH of the concrete surface as well as on trophic properties (e.g., autotrophic or mixotrophic) and on the ability of the SOB to utilize different sulfur compounds (e.g., H 2 S, S 0 , and S 2 O 3 2؊). In addition, diverse heterotrophic bacterial species (e.g., halo-tolerant, neutrophilic, and acidophilic bacteria) were associated with these SOB. The microbial succession of these microorganisms was involved in the colonization of the concrete and the production of sulfuric acid. Furthermore, the vertical distribution of microbial community members revealed that A. thiooxidans was the most dominant throughout the heavily corroded concrete (gypsum) layer and that A. thiooxidans was most abundant at the highest surface (1.5-mm) layer and decreased logarithmically with depth because of oxygen and H 2 S transport limitations. This suggested that the production of sulfuric acid by A. thiooxidans occurred mainly on the concrete surface and the sulfuric acid produced penetrated through the corroded concrete layer and reacted with the sound concrete below.Concrete corrosion has an enormous economic impact worldwide, especially when the replacement or repair of municipal sewer systems is required. Concrete corrosion is the result of several causes such as carbonation, chloride erosion, and other chemical reactions. In sewer systems and wastewater treatment facilities where high concentrations of hydrogen sulfide, moisture, and oxygen are present in the atmosphere, the deterioration of concrete is caused mainly by biogenic acid (i.e., sulfuric acid) and is known as microbially induced concrete corrosion (MICC). The biogenic acid is generated by various microbial species and complex mechanisms. The general mechanism for the sulfuric acidcaused corrosion of sewer systems has been described in the literature (17, 36). In the first step, hydrogen sulfide (H 2 S) is produced by sulfate-reducing bacteria under anaerobic conditions in sewer pipes. This hydrogen sulfide enters the sewer atmosphere by v...

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Section: Succession Of Concrete Corrosion and Microbial Communitycontrasting

confidence: 59%

Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

Okabe

Odagiri

Ito

et al. 2007

Appl Environ Microbiol

305

238

View full text Add to dashboard Cite

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“…Thiobacillus concretivorus (as he termed the strain of Thiobacillus found to attack concrete) did not convert the hydrogen sulfide directly into sulfuric acid but only free sulfur or other forms of utilisable sulfur compounds including thiosulfate [38]. In characterising the strain Thiobacillus thiooxidans, Waksman & Joffe [39] and Nica et al [38] also stated that hydrogen sulfide and other sulfides are not used directly by the sulfur-oxidising organism.…”

Section: Thiooxidans T Intermedius T Perometabolis T Novellus mentioning

confidence: 99%

“…In characterising the strain Thiobacillus thiooxidans, Waksman & Joffe [39] and Nica et al [38] also stated that hydrogen sulfide and other sulfides are not used directly by the sulfur-oxidising organism.…”

Section: Thiooxidans T Intermedius T Perometabolis T Novellus mentioning

confidence: 99%

“…Some of these strains are categorised into 'acid-preferring' acidophilic sulfur-oxidising microorganisms (ASOM), such as T. thiooxidans and 'neutral-preferring' neutrophilic sulfur-oxidising microorganisms (NSOM), such as T. intermedius [14,38]. It is proposed that different strains of neutrophilic bacteria colonise the surface of the concrete as its pH depresses from approximately a value of 8 to around a value of 6 through their production of sulfuric acid [23].…”

Section: Thiooxidans T Intermedius T Perometabolis T Novellus mentioning

confidence: 99%

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Biochemical attack on concrete in wastewater applications: A state of the art review

O’Connell

McNally

Richardson

2010

Cement and Concrete Composites

221

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Publication informationCement and Concrete Composites, 32 (7) AbstractThe costs associated with the provision and maintenance of drinking water and wastewater infrastructure represents a significant financial demand worldwide. Maintenance costs are disproportionately high, indicating a lack of adequate durability. There remains a lack of consensus on degradation mechanisms, the performance of various cement types, the role of bacteria in the corrosion process associated with wastewater applications and testing methodologies. This paper presents a review of the literature, outlining the various research approaches undertaken in an effort to address this problem. The findings of these varying approaches are compared, and the different strategies employed are compiled and discussed. It is proposed that a key step in advancing the understanding of the associated deterioration mechanism is a combined approach that considers the interaction between biological and chemical processes. If this can be achieved then steps can be taken to establishing a performance-based approach for specifying concrete in these harsh service conditions.

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“…Bacteria activity leads to sulphur circulation in a system and in consequence to production of sulphuric acid causing concrete corrosion. It is thought that the following bacteria: Thiobacillus thooxidans, Thiobacillus neapolitanus and Thiobacillus intermedium are responsible for concrete corrosion [6][7][8]. The concrete deterioration mechanism related to bacteria activity, for example Thiobacillus, consists in oxidizing hydrogen sulphide to sulphuric acid that causes acid sulphate corrosion.…”

Section: Introductionmentioning

confidence: 99%

Biological and chemical corrosion of cement materials modified with polymer

Stanaszek-Tomal¹,

Fiertak²

2015

Bulletin of the Polish Academy of Sciences Technical Sciences

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Abstract. The article presents the effect of the addition of polymer to mortars with CEMI and its influence on durability under conditions of sulfuric acid or nitric and sulphur bacteria or nitrogen (nitrification and denitrification). Both acids corresponds to the products of metabolism of bacteria Acidithiobacillus thiooxidans and Thiobacillus denitrificans, Paracoccus denitrificans by the literature is about 0.15 mmol/dm 3 . The changes in tightness materials studied by determining moisture mass and absorption. Corrosion processes were identified by examination in a scanning microscope equipped with an X-ray microanalyzer and a mercury porosimeter. The research results presented showed that the solution has a significantly weaker effect on the composite cement and cement-polymer compared with the action of bacteria. The action of both environments caused two opposing processes: unsealing the structure and deposition of corrosion products.

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Isolation and characterization of microorganisms involved in the biodeterioration of concrete in sewers

Cited by 135 publications

References 19 publications

Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

Biochemical attack on concrete in wastewater applications: A state of the art review

Biological and chemical corrosion of cement materials modified with polymer

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