Isolation and characterization of microsatellite loci from the forage species <i>Arachis pintoi</i> (Genus <i>Arachis</i>)

Palmieri, Darío Abel; Hoshino, Andrea Akemi; Bravo, Juliana Pereira; Lopes, Catalina Romero; Gimenes, Marcos Aparecido

doi:10.1046/j.1471-8286.2002.00317.x

Cited by 40 publications

(26 citation statements)

References 5 publications

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“…As expected, H O (0.017) was much less than the H E (0.479). Such a high H E over H O were observed in the previous studies in groundnut (Palmieri et al, 2002) and chickpea (Sethy et al, 2006). …”

Section: Resultssupporting

confidence: 75%

Utility of AhTE Markers for Genetic and Genomic Studies in Groundnut (Arachis hypogaea L.)

Hake¹,

Bhat²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 75%

Utility of AhTE Markers for Genetic and Genomic Studies in Groundnut (Arachis hypogaea L.)

Hake¹,

Bhat²

2017

Int.J.Curr.Microbiol.App.Sci

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“…The consensus LG2 and LG8 had 8 and 4 primers each, LG3, LG4, LG6, LG7 and LG11 had 3 primers each, LG1, LG9, LG10 and LG13 had one primer each, and 3 unmapped primers were used ( Table 2). The primers used in the study were obtained from different sources as listed, AH series (Hopkins et al 1999), PGP series (Ferguson et al 2004a), PGS series (Ferguson et al 2004b), PM series (He et al 2003), RN17F12, ML1G04, Seq2H11, TC series, and gi-series (Moretzsohn et al 2005), PMc series (He et al 2005), and Ap series (Palmieri et al 2002;Palmieri et al 2005). …”

Section: Ssr Primer Pairsmentioning

confidence: 99%

A first insight into population structure and linkage disequilibrium in the US peanut minicore collection

et al. 2011

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Knowledge of genetic diversity, population structure, and degree of linkage disequilibrium (LD) in target association mapping populations is of great importance and is a prerequisite for LD-based mapping. In the present study, 96 genotypes comprising 92 accessions of the US peanut minicore collection, a component line of the tetraploid variety Florunner, diploid progenitors A. duranensis (AA) and A. ipae¨nsis (BB), and synthetic amphidiploid accession TxAG-6 were investigated with 392 simple sequence repeat (SSR) marker bands amplified using 32 highly-polymorphic SSR primer pairs. Both distance-and model-based (Bayesian) cluster analysis revealed the presence of structured diversity. In general, the wild-species accessions and the synthetic amphidiploid grouped separately from most minicore accessions except for COC155, and were eliminated from most subsequent analyses. UPGMA analysis divided the population into four subgroups, two major subgroups representing subspecies fastigiata and hypogaea, a third group containing individuals from each subspecies or possibly of mixed ancestry, and a fourth group, either consisting of COC155 alone if wild species were excluded, or of COC155, the diploid species, and the synthetic amphidiploid. Modelbased clustering identified four subgroups-one each for fastigiata and hypogaea subspecies, a third consisting of individuals of both subspecies or of mixed ancestry predominantly from Africa or Asia, and a fourth group, consisting of individuals predominantly of var fastigiata, peruviana, and aequatoriana accessions from South America, including COC155. Analysis of molecular variance (AMOVA) revealed statistically-significant (P \ 0.0001) genetic variance of 16.87% among subgroups. A total of 4.85% of SSR marker pairs revealed significant LD (at r 2 C 0.1). Of the syntenic marker pairs separated by distances \ 10 cM, 11-20 cM, 21-50 cM, and [ 50 cM, 19.33, 5.19, 6.25 and 5.29% of marker pairs were found in strong LD (P B 0.01), in accord with LD extending to great distances in self pollinated crops. A threshold value of r 2 [ 0.035 was found to distinguish mean r 2 values of linkage distance groups statistically from the mean r 2 values of unlinked markers; LD was found to extend to 10 cM over the entire minicore collection by this criterion. However, there were large differences in r 2 values among marker pairs even among tightly-linked markers. The implications of these findings with regard to the possibility of using Vikas Belamkar-This work was performed in partial fulfillment of the requirements for the Master of Science degree.

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“…Of these, four loci were developed based on specific sequences for A. hypogaea L. (Ah) (Gimenes et al, 2007), 10 were developed for A. pintoi (Ap) (Palmieri et al, 2002), and three were developed for Arachis glabrata Benth. (Ag) .…”

Section: Dna Extraction and Microsatellite Locimentioning

confidence: 99%

“…Microsatellite markers (simple sequence repeats, SSRs) have been successfully used in characterizing plant diversity (Jones et al, 2009), and have detected high variability among the accessions of section Caulorrhizae (Palmieri et al, 2002;Gimenes et al, 2007). Microsatellite loci exhibit high indices of polymorphism and transferability within the genus Arachis, allowing their use in inter-and intraspecific genetic studies (Palmieri et al, 2002;Moretzsohn et al, 2005;Bravo et al, 2006;Gimenes et al, 2007;Angelici et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of the forage peanut in the Jequitinhonha, São Francisco, and Paranã River valleys of Brazil

et al. 2016

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ABSTRACT.Arachis pintoi and A. repens are legumes with a high forage value that are used to feed ruminants in consortium systems. Not only do they increase the persistence and quality of pastures, they are also used for ornamental and green cover. and eight of A. repens were analyzed using 17 microsatellites, and the observed heterozygosity (H O ), expected heterozygosity (H E ), number of alleles per locus, discriminatory power, and polymorphism information content were all estimated. Ten loci (58.8%) were polymorphic, and 125 alleles were found in total. The H E ranged from 0.30 to 0.94, and H O values ranged from 0.03 to 0.88. By using Bayesian analysis, the accessions were genetically differentiated into three gene pools. Neither the unweighted pair group method with arithmetic mean nor a neighbor-joining analysis clustered samples into species, origin, or collection area. These results reveal a very weak genetic structure that does not form defined clusters, and that there is a high degree of similarity between the two species.

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Isolation and characterization of microsatellite loci from the forage species Arachis pintoi (Genus Arachis)

Cited by 40 publications

References 5 publications

Utility of AhTE Markers for Genetic and Genomic Studies in Groundnut (Arachis hypogaea L.)

Utility of AhTE Markers for Genetic and Genomic Studies in Groundnut (Arachis hypogaea L.)

A first insight into population structure and linkage disequilibrium in the US peanut minicore collection

Genetic diversity of the forage peanut in the Jequitinhonha, São Francisco, and Paranã River valleys of Brazil

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